Restriction map for
pF1KB6418
(Linear) (Six-base) MAPSORT of: KIBB6418.seq Check: 9657 from: 1 to: 1011
KIBB6418.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 320 1011
Size: 320 691
AleI CACnn'nnGTG
Cuts at: 0 67 70 1011
Size: 67 3 941
Fragments arranged by size:
941 67 3
AlwNI CAG_nnn'CTG
Cuts at: 0 180 1011
Size: 180 831
ApoI r'AATT_y
Cuts at: 0 413 446 1011
Size: 413 33 565
Fragments arranged by size:
565 413 33
AvaI C'yCGr_G
Cuts at: 0 466 1011
Size: 466 545
BamHI G'GATC_C
Cuts at: 0 932 1011
Size: 932 79
BanI G'GyrC_C
Cuts at: 0 703 810 1011
Size: 703 107 201
Fragments arranged by size:
703 201 107
BanII G_rGCy'C
Cuts at: 0 10 383 467 623 1011
Size: 10 373 84 156 388
Fragments arranged by size:
388 373 156 84 10
BbeI G_GCGC'C
Cuts at: 0 707 1011
Size: 707 304
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 1011
Size: 635 376
BciVI GTATCCnnnnn_n'
Cuts at: 0 318 1011
Size: 318 693
BglI GCCn_nnn'nGGC
Cuts at: 0 832 1011
Size: 832 179
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 1011
Size: 939 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1011
Size: 985 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1011
Size: 37 974
BsaHI Gr'CG_yC
Cuts at: 0 704 1011
Size: 704 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 758 788 1011
Size: 758 30 223
Fragments arranged by size:
758 223 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 484 1011
Size: 202 282 527
Fragments arranged by size:
527 282 202
BseYI C'CCAG_C
Cuts at: 0 213 394 1011
Size: 213 181 617
Fragments arranged by size:
617 213 181
BsiHKAI G_wGCw'C
Cuts at: 0 10 383 467 821 1011
Size: 10 373 84 354 190
Fragments arranged by size:
373 354 190 84 10
Bsp1286I G_dGCh'C
Cuts at: 0 10 383 467 623 821 1011
Size: 10 373 84 156 198 190
Fragments arranged by size:
373 198 190 156 84 10
BsrBI CCG'CTC
Cuts at: 0 969 1011
Size: 969 42
BsrFI r'CCGG_y
Cuts at: 0 742 1011
Size: 742 269
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 1011
Size: 488 523
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 699 777 849 1011
Size: 72 627 78 72 162
Fragments arranged by size:
627 162 78 72 72
BstYI r'GATC_y
Cuts at: 0 932 1011
Size: 932 79
BtgI C'CryG_G
Cuts at: 0 68 1011
Size: 68 943
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 364 1011
Size: 364 647
BtsI GCAGTG_nn'
Cuts at: 0 486 962 1011
Size: 486 476 49
Fragments arranged by size:
486 476 49
EaeI y'GGCC_r
Cuts at: 0 74 149 204 356 740 1011
Size: 74 75 55 152 384 271
Fragments arranged by size:
384 271 152 75 74 55
EarI CTCTTCn'nnn_
Cuts at: 0 511 1011
Size: 511 500
EcoICRI GAG'CTC
Cuts at: 0 8 381 465 1011
Size: 8 373 84 546
Fragments arranged by size:
546 373 84 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1011
Size: 985 26
EcoRI G'AATT_C
Cuts at: 0 413 446 1011
Size: 413 33 565
Fragments arranged by size:
565 413 33
HaeII r_GCGC'y
Cuts at: 0 707 1011
Size: 707 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 859 891 1011
Size: 595 32 232 32 120
Fragments arranged by size:
595 232 120 32 32
KasI G'GCGC_C
Cuts at: 0 703 1011
Size: 703 308
MscI TGG'CCA
Cuts at: 0 358 1011
Size: 358 653
MslI CAynn'nnrTG
Cuts at: 0 67 70 697 866 1011
Size: 67 3 627 169 145
Fragments arranged by size:
627 169 145 67 3
MspA1I CmG'CkG
Cuts at: 0 949 1011
Size: 949 62
NaeI GCC'GGC
Cuts at: 0 744 1011
Size: 744 267
NarI GG'CG_CC
Cuts at: 0 704 1011
Size: 704 307
NgoMIV G'CCGG_C
Cuts at: 0 742 1011
Size: 742 269
NspI r_CATG'y
Cuts at: 0 592 1011
Size: 592 419
PasI CC'CwG_GG
Cuts at: 0 634 1011
Size: 634 377
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 525 568 1011
Size: 180 345 43 443
Fragments arranged by size:
443 345 180 43
PsiI TTA'TAA
Cuts at: 0 421 1011
Size: 421 590
PspXI vC'TCGA_Gb
Cuts at: 0 466 1011
Size: 466 545
PstI C_TGCA'G
Cuts at: 0 794 1011
Size: 794 217
SacI G_AGCT'C
Cuts at: 0 10 383 467 1011
Size: 10 373 84 544
Fragments arranged by size:
544 373 84 10
SfcI C'TryA_G
Cuts at: 0 790 1011
Size: 790 221
SfoI GGC'GCC
Cuts at: 0 705 1011
Size: 705 306
SmlI C'TyrA_G
Cuts at: 0 52 466 1011
Size: 52 414 545
Fragments arranged by size:
545 414 52
StyI C'CwwG_G
Cuts at: 0 827 1011
Size: 827 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 425 783 1011
Size: 425 358 228
Fragments arranged by size:
425 358 228
TatI w'GTAC_w
Cuts at: 0 107 1011
Size: 107 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 758 790 1011
Size: 758 32 221
Fragments arranged by size:
758 221 32
XhoI C'TCGA_G
Cuts at: 0 466 1011
Size: 466 545
XmnI GAAnn'nnTTC
Cuts at: 0 446 877 1011
Size: 446 431 134
Fragments arranged by size:
446 431 134
Enzymes that do cut:
AccI AleI AlwNI ApoI AvaI BamHI BanI BanII
BbeI BbsI BciVI BglI BplI BpmI BpuEI BsaHI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoRI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PflMI PsiI PspXI
PstI SacI SfcI SfoI SmlI StyI TaqII TatI
TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XmaI
ZraI