Restriction map for
pF1KB6419
(Linear) (Six-base) MAPSORT of: KIBB6419.seq Check: 4778 from: 1 to: 927
KIBB6419.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 927
Size: 156 771
AflIII A'CryG_T
Cuts at: 0 774 927
Size: 774 153
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 761 793 927
Size: 761 32 134
Fragments arranged by size:
761 134 32
AlwNI CAG_nnn'CTG
Cuts at: 0 750 927
Size: 750 177
AvaI C'yCGr_G
Cuts at: 0 422 927
Size: 422 505
BanI G'GyrC_C
Cuts at: 0 705 927
Size: 705 222
BbsI GAAGACnn'nnnn_
Cuts at: 0 807 927
Size: 807 120
BfrBI ATG'CAT
Cuts at: 0 898 927
Size: 898 29
BglII A'GATC_T
Cuts at: 0 610 927
Size: 610 317
BlpI GC'TnA_GC
Cuts at: 0 596 927
Size: 596 331
BmrI ACTGGGnnnn_n'
Cuts at: 0 917 927
Size: 917 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 872 927
Size: 580 292 55
Fragments arranged by size:
580 292 55
Bpu10I CC'TnA_GC
Cuts at: 0 782 927
Size: 782 145
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 808 927
Size: 655 153 119
Fragments arranged by size:
655 153 119
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 927
Size: 137 790
BsiHKAI G_wGCw'C
Cuts at: 0 888 927
Size: 888 39
BsmI GAATG_Cn'
Cuts at: 0 898 927
Size: 898 29
Bsp1286I G_dGCh'C
Cuts at: 0 888 927
Size: 888 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 927
Size: 336 591
BstYI r'GATC_y
Cuts at: 0 192 610 927
Size: 192 418 317
Fragments arranged by size:
418 317 192
BtsI GCAGTG_nn'
Cuts at: 0 748 927
Size: 748 179
DraI TTT'AAA
Cuts at: 0 815 927
Size: 815 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 580 872 927
Size: 156 424 292 55
Fragments arranged by size:
424 292 156 55
EcoO109I rG'GnC_Cy
Cuts at: 0 843 927
Size: 843 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 186 927
Size: 186 741
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 555 587 927
Size: 71 32 452 32 340
Fragments arranged by size:
452 340 71 32 32
HincII GTy'rAC
Cuts at: 0 204 691 927
Size: 204 487 236
Fragments arranged by size:
487 236 204
HindIII A'AGCT_T
Cuts at: 0 446 718 927
Size: 446 272 209
Fragments arranged by size:
446 272 209
MslI CAynn'nnrTG
Cuts at: 0 682 927
Size: 682 245
NdeI CA'TA_TG
Cuts at: 0 765 927
Size: 765 162
NsiI A_TGCA'T
Cuts at: 0 900 927
Size: 900 27
NspI r_CATG'y
Cuts at: 0 778 927
Size: 778 149
PciI A'CATG_T
Cuts at: 0 774 927
Size: 774 153
PpuMI rG'GwC_Cy
Cuts at: 0 843 927
Size: 843 84
PstI C_TGCA'G
Cuts at: 0 515 755 927
Size: 515 240 172
Fragments arranged by size:
515 240 172
SfcI C'TryA_G
Cuts at: 0 511 751 889 927
Size: 511 240 138 38
Fragments arranged by size:
511 240 138 38
SmlI C'TyrA_G
Cuts at: 0 422 634 787 927
Size: 422 212 153 140
Fragments arranged by size:
422 212 153 140
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 927
Size: 360 567
XhoI C'TCGA_G
Cuts at: 0 422 927
Size: 422 505
XmnI GAAnn'nnTTC
Cuts at: 0 263 927
Size: 263 664
Enzymes that do cut:
AcuI AflIII AloI AlwNI AvaI BanI BbsI BfrBI
BglII BlpI BmrI BpmI Bpu10I BpuEI BseRI BsiHKAI
BsmI Bsp1286I BstXI BstYI BtsI DraI Eco57MI EcoO109I
FalI Hin4I HincII HindIII MslI NdeI NsiI NspI
PciI PpuMI PstI SfcI SmlI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BciVI BclI BglI Bme1580I BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XmaI ZraI