Restriction map for
pF1KB6421
(Linear) (Six-base) MAPSORT of: KIBB6421.seq Check: 333 from: 1 to: 927
KIBB6421.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 927
Size: 878 49
AflII C'TTAA_G
Cuts at: 0 535 927
Size: 535 392
AflIII A'CryG_T
Cuts at: 0 646 927
Size: 646 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 275 927
Size: 94 181 652
Fragments arranged by size:
652 181 94
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 260 292 927
Size: 260 32 635
Fragments arranged by size:
635 260 32
ApoI r'AATT_y
Cuts at: 0 309 427 595 899 927
Size: 309 118 168 304 28
Fragments arranged by size:
309 304 168 118 28
AvaI C'yCGr_G
Cuts at: 0 124 161 927
Size: 124 37 766
Fragments arranged by size:
766 124 37
BanI G'GyrC_C
Cuts at: 0 30 927
Size: 30 897
BbsI GAAGACnn'nnnn_
Cuts at: 0 400 927
Size: 400 527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 95 129 927
Size: 95 34 798
Fragments arranged by size:
798 95 34
Bme1580I G_kGCm'C
Cuts at: 0 534 927
Size: 534 393
Bpu10I CC'TnA_GC
Cuts at: 0 170 365 927
Size: 170 195 562
Fragments arranged by size:
562 195 170
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 927
Size: 142 785
BseYI C'CCAG_C
Cuts at: 0 53 927
Size: 53 874
BsiEI CG_ry'CG
Cuts at: 0 115 927
Size: 115 812
BsiHKAI G_wGCw'C
Cuts at: 0 713 927
Size: 713 214
Bsp1286I G_dGCh'C
Cuts at: 0 534 713 927
Size: 534 179 214
Fragments arranged by size:
534 214 179
BstAPI GCAn_nnn'nTGC
Cuts at: 0 352 927
Size: 352 575
BstYI r'GATC_y
Cuts at: 0 748 861 927
Size: 748 113 66
Fragments arranged by size:
748 113 66
BtgI C'CryG_G
Cuts at: 0 38 179 927
Size: 38 141 748
Fragments arranged by size:
748 141 38
BtsI GCAGTG_nn'
Cuts at: 0 113 353 927
Size: 113 240 574
Fragments arranged by size:
574 240 113
ClaI AT'CG_AT
Cuts at: 0 115 927
Size: 115 812
EaeI y'GGCC_r
Cuts at: 0 144 927
Size: 144 783
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 927
Size: 878 49
EcoRI G'AATT_C
Cuts at: 0 427 927
Size: 427 500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 927
Size: 561 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 817 849 927
Size: 754 32 31 32 78
Fragments arranged by size:
754 78 32 32 31
HindIII A'AGCT_T
Cuts at: 0 483 543 927
Size: 483 60 384
Fragments arranged by size:
483 384 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 546 927
Size: 357 189 381
Fragments arranged by size:
381 357 189
MscI TGG'CCA
Cuts at: 0 146 927
Size: 146 781
MslI CAynn'nnrTG
Cuts at: 0 761 927
Size: 761 166
MspA1I CmG'CkG
Cuts at: 0 507 588 927
Size: 507 81 339
Fragments arranged by size:
507 339 81
NcoI C'CATG_G
Cuts at: 0 38 179 927
Size: 38 141 748
Fragments arranged by size:
748 141 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 412 927
Size: 412 515
PfoI T'CCnGG_A
Cuts at: 0 656 927
Size: 656 271
PsiI TTA'TAA
Cuts at: 0 638 927
Size: 638 289
PvuI CG_AT'CG
Cuts at: 0 115 927
Size: 115 812
PvuII CAG'CTG
Cuts at: 0 507 927
Size: 507 420
SmaI CCC'GGG
Cuts at: 0 163 927
Size: 163 764
SmlI C'TyrA_G
Cuts at: 0 535 927
Size: 535 392
SpeI A'CTAG_T
Cuts at: 0 397 927
Size: 397 530
StyI C'CwwG_G
Cuts at: 0 38 179 232 927
Size: 38 141 53 695
Fragments arranged by size:
695 141 53 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 638 670 927
Size: 638 32 257
Fragments arranged by size:
638 257 32
XbaI T'CTAG_A
Cuts at: 0 751 927
Size: 751 176
XmaI C'CCGG_G
Cuts at: 0 161 927
Size: 161 766
XmnI GAAnn'nnTTC
Cuts at: 0 431 899 927
Size: 431 468 28
Fragments arranged by size:
468 431 28
Enzymes that do cut:
AcuI AflII AflIII AlfI AloI ApoI AvaI BanI
BbsI BcgI Bme1580I Bpu10I BseRI BseYI BsiEI BsiHKAI
Bsp1286I BstAPI BstYI BtgI BtsI ClaI EaeI Eco57MI
EcoRI FalI Hin4I HindIII MmeI MscI MslI MspA1I
NcoI PflMI PfoI PsiI PvuI PvuII SmaI SmlI
SpeI StyI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XcmI XhoI ZraI