Restriction map for
pF1KB6422
(Linear) (Six-base) MAPSORT of: KIBB6422.seq Check: 8916 from: 1 to: 1017
KIBB6422.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 436 1017
Size: 436 581
AccI GT'mk_AC
Cuts at: 0 662 1017
Size: 662 355
Acc65I G'GTAC_C
Cuts at: 0 490 1017
Size: 490 527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 983 989 1017
Size: 23 960 6 28
Fragments arranged by size:
960 28 23 6
AlwNI CAG_nnn'CTG
Cuts at: 0 523 932 1017
Size: 523 409 85
Fragments arranged by size:
523 409 85
ApoI r'AATT_y
Cuts at: 0 218 271 382 1017
Size: 218 53 111 635
Fragments arranged by size:
635 218 111 53
AseI AT'TA_AT
Cuts at: 0 986 1017
Size: 986 31
AvaI C'yCGr_G
Cuts at: 0 575 696 1017
Size: 575 121 321
Fragments arranged by size:
575 321 121
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 26 59 1017
Size: 26 33 958
Fragments arranged by size:
958 33 26
BanI G'GyrC_C
Cuts at: 0 490 1017
Size: 490 527
BbsI GAAGACnn'nnnn_
Cuts at: 0 403 949 1017
Size: 403 546 68
Fragments arranged by size:
546 403 68
BbvCI CC'TCA_GC
Cuts at: 0 227 1017
Size: 227 790
BfrBI ATG'CAT
Cuts at: 0 883 1017
Size: 883 134
BglI GCCn_nnn'nGGC
Cuts at: 0 507 1017
Size: 507 510
BglII A'GATC_T
Cuts at: 0 404 1017
Size: 404 613
BmrI ACTGGGnnnn_n'
Cuts at: 0 401 1017
Size: 401 616
BmtI G_CTAG'C
Cuts at: 0 421 1017
Size: 421 596
Bpu10I CC'TnA_GC
Cuts at: 0 227 1017
Size: 227 790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1017
Size: 797 220
BsaI GGTCTCn'nnnn_
Cuts at: 0 541 1017
Size: 541 476
BseYI C'CCAG_C
Cuts at: 0 602 1017
Size: 602 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 436 1017
Size: 436 581
BsrDI GCAATG_nn'
Cuts at: 0 11 1017
Size: 11 1006
BsrGI T'GTAC_A
Cuts at: 0 473 1017
Size: 473 544
BstYI r'GATC_y
Cuts at: 0 358 404 1017
Size: 358 46 613
Fragments arranged by size:
613 358 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 844 858 1017
Size: 844 14 159
Fragments arranged by size:
844 159 14
BtsI GCAGTG_nn'
Cuts at: 0 94 260 524 552 649 791 1017
Size: 94 166 264 28 97 142 226
Fragments arranged by size:
264 226 166 142 97 94 28
EaeI y'GGCC_r
Cuts at: 0 734 1017
Size: 734 283
EarI CTCTTCn'nnn_
Cuts at: 0 909 1017
Size: 909 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 23 983 989 1017
Size: 23 960 6 28
Fragments arranged by size:
960 28 23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 860 1017
Size: 860 157
EcoRI G'AATT_C
Cuts at: 0 271 382 1017
Size: 271 111 635
Fragments arranged by size:
635 271 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 519 551 854 886 1017
Size: 343 32 144 32 303 32 131
Fragments arranged by size:
343 303 144 131 32 32 32
HindIII A'AGCT_T
Cuts at: 0 54 1017
Size: 54 963
KpnI G_GTAC'C
Cuts at: 0 494 1017
Size: 494 523
MscI TGG'CCA
Cuts at: 0 736 1017
Size: 736 281
MslI CAynn'nnrTG
Cuts at: 0 370 1017
Size: 370 647
NdeI CA'TA_TG
Cuts at: 0 570 1017
Size: 570 447
NheI G'CTAG_C
Cuts at: 0 417 1017
Size: 417 600
NsiI A_TGCA'T
Cuts at: 0 885 1017
Size: 885 132
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1017
Size: 144 873
PfoI T'CCnGG_A
Cuts at: 0 554 1017
Size: 554 463
PspXI vC'TCGA_Gb
Cuts at: 0 575 1017
Size: 575 442
SmlI C'TyrA_G
Cuts at: 0 575 812 1017
Size: 575 237 205
Fragments arranged by size:
575 237 205
SspI AAT'ATT
Cuts at: 0 186 1017
Size: 186 831
TatI w'GTAC_w
Cuts at: 0 473 1017
Size: 473 544
XhoI C'TCGA_G
Cuts at: 0 575 1017
Size: 575 442
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApoI AseI AvaI
BaeI BanI BbsI BbvCI BfrBI BglI BglII BmrI
BmtI Bpu10I BpuEI BsaI BseYI BspMI BsrDI BsrGI
BstYI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoRI
Hin4I HindIII KpnI MscI MslI NdeI NheI NsiI
PflMI PfoI PspXI SmlI SspI TatI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI
BanII BbeI BcgI BciVI BclI BlpI Bme1580I BmgBI
BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NotI NruI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI StyI SwaI TaqII TstI Tth111I
XbaI XcmI XmaI XmnI ZraI