Restriction map for
pF1KB6423
(Linear) (Six-base) MAPSORT of: KIBB6423.seq Check: 3703 from: 1 to: 927
KIBB6423.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
Acc65I G'GTAC_C
Cuts at: 0 652 799 927
Size: 652 147 128
Fragments arranged by size:
652 147 128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 770 927
Size: 218 552 157
Fragments arranged by size:
552 218 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 209 390 927
Size: 209 181 537
Fragments arranged by size:
537 209 181
ApoI r'AATT_y
Cuts at: 0 267 827 927
Size: 267 560 100
Fragments arranged by size:
560 267 100
BanI G'GyrC_C
Cuts at: 0 652 799 927
Size: 652 147 128
Fragments arranged by size:
652 147 128
BanII G_rGCy'C
Cuts at: 0 320 432 495 927
Size: 320 112 63 432
Fragments arranged by size:
432 320 112 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 927
Size: 339 588
BciVI GTATCCnnnnn_n'
Cuts at: 0 314 927
Size: 314 613
BfrBI ATG'CAT
Cuts at: 0 186 927
Size: 186 741
BglI GCCn_nnn'nGGC
Cuts at: 0 46 904 922 927
Size: 46 858 18 5
Fragments arranged by size:
858 46 18 5
BlpI GC'TnA_GC
Cuts at: 0 765 927
Size: 765 162
BmtI G_CTAG'C
Cuts at: 0 544 847 927
Size: 544 303 80
Fragments arranged by size:
544 303 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 413 758 927
Size: 226 187 345 169
Fragments arranged by size:
345 226 187 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 427 598 927
Size: 262 165 171 329
Fragments arranged by size:
329 262 171 165
BsiHKAI G_wGCw'C
Cuts at: 0 320 927
Size: 320 607
BsmI GAATG_Cn'
Cuts at: 0 638 927
Size: 638 289
Bsp1286I G_dGCh'C
Cuts at: 0 320 432 495 927
Size: 320 112 63 432
Fragments arranged by size:
432 320 112 63
BsrDI GCAATG_nn'
Cuts at: 0 367 927
Size: 367 560
BsrFI r'CCGG_y
Cuts at: 0 122 927
Size: 122 805
BstBI TT'CG_AA
Cuts at: 0 440 927
Size: 440 487
BstXI CCAn_nnnn'nTGG
Cuts at: 0 454 927
Size: 454 473
Bsu36I CC'TnA_GG
Cuts at: 0 854 927
Size: 854 73
BtgI C'CryG_G
Cuts at: 0 453 835 927
Size: 453 382 92
Fragments arranged by size:
453 382 92
BtsI GCAGTG_nn'
Cuts at: 0 379 677 927
Size: 379 298 250
Fragments arranged by size:
379 298 250
DraIII CAC_nnn'GTG
Cuts at: 0 528 927
Size: 528 399
EcoICRI GAG'CTC
Cuts at: 0 318 927
Size: 318 609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 226 413 758 770 927
Size: 218 8 187 345 12 157
Fragments arranged by size:
345 218 187 157 12 8
EcoO109I rG'GnC_Cy
Cuts at: 0 481 858 927
Size: 481 377 69
Fragments arranged by size:
481 377 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 305 337 482 514 927
Size: 305 32 145 32 413
Fragments arranged by size:
413 305 145 32 32
KpnI G_GTAC'C
Cuts at: 0 656 803 927
Size: 656 147 124
Fragments arranged by size:
656 147 124
MslI CAynn'nnrTG
Cuts at: 0 187 372 452 927
Size: 187 185 80 475
Fragments arranged by size:
475 187 185 80
MspA1I CmG'CkG
Cuts at: 0 796 889 927
Size: 796 93 38
Fragments arranged by size:
796 93 38
NcoI C'CATG_G
Cuts at: 0 453 927
Size: 453 474
NdeI CA'TA_TG
Cuts at: 0 188 296 927
Size: 188 108 631
Fragments arranged by size:
631 188 108
NheI G'CTAG_C
Cuts at: 0 540 843 927
Size: 540 303 84
Fragments arranged by size:
540 303 84
NsiI A_TGCA'T
Cuts at: 0 188 927
Size: 188 739
PasI CC'CwG_GG
Cuts at: 0 330 708 927
Size: 330 378 219
Fragments arranged by size:
378 330 219
PpuMI rG'GwC_Cy
Cuts at: 0 481 927
Size: 481 446
PstI C_TGCA'G
Cuts at: 0 557 927
Size: 557 370
PvuII CAG'CTG
Cuts at: 0 796 927
Size: 796 131
SacI G_AGCT'C
Cuts at: 0 320 927
Size: 320 607
SanDI GG'GwC_CC
Cuts at: 0 481 927
Size: 481 446
SfcI C'TryA_G
Cuts at: 0 553 927
Size: 553 374
StyI C'CwwG_G
Cuts at: 0 453 927
Size: 453 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 927
Size: 389 32 506
Fragments arranged by size:
506 389 32
XmnI GAAnn'nnTTC
Cuts at: 0 270 830 927
Size: 270 560 97
Fragments arranged by size:
560 270 97
Enzymes that do cut:
Acc65I AcuI AlfI ApoI BanI BanII BbsI BciVI
BfrBI BglI BlpI BmtI BpmI BsgI BsiHKAI BsmI
Bsp1286I BsrDI BsrFI BstBI BstXI Bsu36I BtgI BtsI
DraIII EcoICRI Eco57MI EcoO109I Hin4I KpnI MslI MspA1I
NcoI NdeI NheI NsiI PasI PpuMI PstI PvuII
SacI SanDI SfcI StyI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BglII Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI MscI NaeI
NarI NgoMIV NotI NruI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI ZraI