(Linear) (Six-base) MAPSORT of: KIBB6424.seq  Check: 8884  from: 1  to: 1020



KIBB6424.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     904    1020 
   Size:        904     116
 
AccI GT'mk_AC

Cuts at:      0     654    1020 
   Size:        654     366
 
AflIII A'CryG_T

Cuts at:      0     672    1020 
   Size:        672     348
 
AseI AT'TA_AT

Cuts at:      0     235    1020 
   Size:        235     785
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     184    1020 
   Size:        184     836
 
BfrBI ATG'CAT

Cuts at:      0     183     270    1020 
   Size:        183      87     750

  Fragments arranged by size: 

                 750     183      87
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     551    1020 
   Size:        551     469
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1020 
   Size:        572     448
 
Bpu10I CC'TnA_GC

Cuts at:      0     951    1020 
   Size:        951      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1020 
   Size:        206     814
 
BsaHI Gr'CG_yC

Cuts at:      0     846    1020 
   Size:        846     174
 
BseYI C'CCAG_C

Cuts at:      0     521    1020 
   Size:        521     499
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     788     932    1020 
   Size:        788     144      88

  Fragments arranged by size: 

                 788     144      88
 
BsmI GAATG_Cn'

Cuts at:      0     546    1020 
   Size:        546     474
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     853    1020 
   Size:        853     167
 
BspHI T'CATG_A

Cuts at:      0     795     930    1020 
   Size:        795     135      90

  Fragments arranged by size: 

                 795     135      90
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     904    1020 
   Size:        904     116
 
BsrFI r'CCGG_y

Cuts at:      0     749    1020 
   Size:        749     271
 
EarI CTCTTCn'nnn_

Cuts at:      0     624    1020 
   Size:        624     396
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1020 
   Size:        572     448
 
EcoO109I rG'GnC_Cy

Cuts at:      0     287    1020 
   Size:        287     733
 
EcoRV GAT'ATC

Cuts at:      0     684    1020 
   Size:        684     336
 
MslI CAynn'nnrTG

Cuts at:      0     976    1020 
   Size:        976      44
 
NaeI GCC'GGC

Cuts at:      0     751    1020 
   Size:        751     269
 
NgoMIV G'CCGG_C

Cuts at:      0     749    1020 
   Size:        749     271
 
NsiI A_TGCA'T

Cuts at:      0     185     272    1020 
   Size:        185      87     748

  Fragments arranged by size: 

                 748     185      87
 
NspI r_CATG'y

Cuts at:      0      74     274     676    1020 
   Size:         74     200     402     344

  Fragments arranged by size: 

                 402     344     200      74
 
PciI A'CATG_T

Cuts at:      0     672    1020 
   Size:        672     348
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     629    1020 
   Size:        629     391
 
PpuMI rG'GwC_Cy

Cuts at:      0     287    1020 
   Size:        287     733
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     129     161    1020 
   Size:        129      32     859

  Fragments arranged by size: 

                 859     129      32
 
SfcI C'TryA_G

Cuts at:      0     824    1020 
   Size:        824     196
 
SmlI C'TyrA_G

Cuts at:      0     221    1020 
   Size:        221     799
 
SphI G_CATG'C

Cuts at:      0     274    1020 
   Size:        274     746
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     285    1020 
   Size:        285     735
 
XmnI GAAnn'nnTTC

Cuts at:      0     819    1020 
   Size:        819     201
 

 Enzymes that do cut:

     AarI     AccI   AflIII     AseI    BciVI    BfrBI     BmrI     BpmI
   Bpu10I    BpuEI    BsaHI    BseYI     BsgI     BsmI    BsmBI    BspHI
    BspMI    BsrFI     EarI  Eco57MI EcoO109I    EcoRV     MslI     NaeI
   NgoMIV     NsiI     NspI     PciI    PflMI    PpuMI     PsrI     SfcI
     SmlI     SphI    TaqII     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI