Restriction map for
pF1KB6425
(Linear) (Six-base) MAPSORT of: KIBB6425.seq Check: 3260 from: 1 to: 930
KIBB6425.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 136 930
Size: 136 794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 930
Size: 113 817
AlwNI CAG_nnn'CTG
Cuts at: 0 210 930
Size: 210 720
ApaI G_GGCC'C
Cuts at: 0 296 930
Size: 296 634
ApoI r'AATT_y
Cuts at: 0 919 930
Size: 919 11
AvaI C'yCGr_G
Cuts at: 0 347 645 930
Size: 347 298 285
Fragments arranged by size:
347 298 285
BanII G_rGCy'C
Cuts at: 0 296 443 616 791 930
Size: 296 147 173 175 139
Fragments arranged by size:
296 175 173 147 139
BbvCI CC'TCA_GC
Cuts at: 0 575 930
Size: 575 355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 587 930
Size: 553 34 343
Fragments arranged by size:
553 343 34
BglI GCCn_nnn'nGGC
Cuts at: 0 430 721 930
Size: 430 291 209
Fragments arranged by size:
430 291 209
BlpI GC'TnA_GC
Cuts at: 0 43 930
Size: 43 887
Bme1580I G_kGCm'C
Cuts at: 0 296 930
Size: 296 634
BmgBI CAC'GTC
Cuts at: 0 227 930
Size: 227 703
BmrI ACTGGGnnnn_n'
Cuts at: 0 694 930
Size: 694 236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 305 573 930
Size: 215 90 268 357
Fragments arranged by size:
357 268 215 90
Bpu10I CC'TnA_GC
Cuts at: 0 138 575 930
Size: 138 437 355
Fragments arranged by size:
437 355 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 373 481 930
Size: 373 108 449
Fragments arranged by size:
449 373 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 296 326 719 749 767 797
Size: 115 30 151 30 393 30 18 30
Cuts at: 797 930
Size: 133
Fragments arranged by size:
393 151 133 115 30 30 30 30
18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 880 930
Size: 880 50
BseYI C'CCAG_C
Cuts at: 0 508 600 756 930
Size: 508 92 156 174
Fragments arranged by size:
508 174 156 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 930
Size: 164 766
BsmBI CGTCTCn'nnnn_
Cuts at: 0 233 930
Size: 233 697
Bsp1286I G_dGCh'C
Cuts at: 0 296 443 616 791 930
Size: 296 147 173 175 139
Fragments arranged by size:
296 175 173 147 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 930
Size: 136 794
BsrDI GCAATG_nn'
Cuts at: 0 767 930
Size: 767 163
BsrFI r'CCGG_y
Cuts at: 0 54 241 279 930
Size: 54 187 38 651
Fragments arranged by size:
651 187 54 38
BssHII G'CGCG_C
Cuts at: 0 6 32 930
Size: 6 26 898
Fragments arranged by size:
898 26 6
BssSI C'ACGA_G
Cuts at: 0 781 930
Size: 781 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 930
Size: 246 684
BtgI C'CryG_G
Cuts at: 0 428 671 716 930
Size: 428 243 45 214
Fragments arranged by size:
428 243 214 45
EaeI y'GGCC_r
Cuts at: 0 477 668 930
Size: 477 191 262
Fragments arranged by size:
477 262 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 215 305 573 930
Size: 113 102 90 268 357
Fragments arranged by size:
357 268 113 102 90
EcoO109I rG'GnC_Cy
Cuts at: 0 292 505 550 722 732 890 930
Size: 292 213 45 172 10 158 40
Fragments arranged by size:
292 213 172 158 45 40 10
EcoRI G'AATT_C
Cuts at: 0 919 930
Size: 919 11
EcoRV GAT'ATC
Cuts at: 0 162 930
Size: 162 768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 538 930
Size: 538 392
HaeII r_GCGC'y
Cuts at: 0 16 43 930
Size: 16 27 887
Fragments arranged by size:
887 27 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 387 419 571 603 930
Size: 115 32 240 32 152 32 327
Fragments arranged by size:
327 240 152 115 32 32 32
MscI TGG'CCA
Cuts at: 0 479 930
Size: 479 451
MspA1I CmG'CkG
Cuts at: 0 259 930
Size: 259 671
NaeI GCC'GGC
Cuts at: 0 56 281 930
Size: 56 225 649
Fragments arranged by size:
649 225 56
NgoMIV G'CCGG_C
Cuts at: 0 54 279 930
Size: 54 225 651
Fragments arranged by size:
651 225 54
NspI r_CATG'y
Cuts at: 0 471 930
Size: 471 459
PasI CC'CwG_GG
Cuts at: 0 374 930
Size: 374 556
PfoI T'CCnGG_A
Cuts at: 0 737 930
Size: 737 193
PpuMI rG'GwC_Cy
Cuts at: 0 505 550 930
Size: 505 45 380
Fragments arranged by size:
505 380 45
PspOMI G'GGCC_C
Cuts at: 0 292 930
Size: 292 638
PstI C_TGCA'G
Cuts at: 0 53 504 930
Size: 53 451 426
Fragments arranged by size:
451 426 53
PvuII CAG'CTG
Cuts at: 0 259 930
Size: 259 671
SbfI CC_TGCA'GG
Cuts at: 0 504 930
Size: 504 426
SfcI C'TryA_G
Cuts at: 0 49 254 311 500 662 930
Size: 49 205 57 189 162 268
Fragments arranged by size:
268 205 189 162 57 49
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 721 930
Size: 721 209
SmaI CCC'GGG
Cuts at: 0 349 930
Size: 349 581
StuI AGG'CCT
Cuts at: 0 409 779 930
Size: 409 370 151
Fragments arranged by size:
409 370 151
StyI C'CwwG_G
Cuts at: 0 296 860 930
Size: 296 564 70
Fragments arranged by size:
564 296 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 767 799 930
Size: 719 32 16 32 131
Fragments arranged by size:
719 131 32 32 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 541 930
Size: 541 389
XmaI C'CCGG_G
Cuts at: 0 347 930
Size: 347 583
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BanII BbvCI
BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspMI
BsrDI BsrFI BssHII BssSI BstXI BtgI EaeI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I MscI MspA1I
NaeI NgoMIV NspI PasI PfoI PpuMI PspOMI PstI
PvuII SbfI SfcI SfiI SmaI StuI StyI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI
BfrBI BglII BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI
BsrGI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI
XmnI ZraI