Restriction map for
pF1KB6427
(Linear) (Six-base) MAPSORT of: KIBB6427.seq Check: 763 from: 1 to: 918
KIBB6427.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 855 918
Size: 855 63
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 292 454 918
Size: 159 133 162 464
Fragments arranged by size:
464 162 159 133
AlwNI CAG_nnn'CTG
Cuts at: 0 30 144 399 918
Size: 30 114 255 519
Fragments arranged by size:
519 255 114 30
ApaLI G'TGCA_C
Cuts at: 0 242 918
Size: 242 676
ApoI r'AATT_y
Cuts at: 0 649 918
Size: 649 269
AvaI C'yCGr_G
Cuts at: 0 297 918
Size: 297 621
AvrII C'CTAG_G
Cuts at: 0 366 918
Size: 366 552
BanII G_rGCy'C
Cuts at: 0 37 482 918
Size: 37 445 436
Fragments arranged by size:
445 436 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 818 918
Size: 818 100
BbvCI CC'TCA_GC
Cuts at: 0 581 918
Size: 581 337
BlpI GC'TnA_GC
Cuts at: 0 31 918
Size: 31 887
Bme1580I G_kGCm'C
Cuts at: 0 246 794 918
Size: 246 548 124
Fragments arranged by size:
548 246 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 239 534 768 918
Size: 239 295 234 150
Fragments arranged by size:
295 239 234 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 625 918
Size: 400 225 293
Fragments arranged by size:
400 293 225
Bpu10I CC'TnA_GC
Cuts at: 0 190 581 918
Size: 190 391 337
Fragments arranged by size:
391 337 190
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 918
Size: 697 221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 918
Size: 329 30 559
Fragments arranged by size:
559 329 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 285 472 655 918
Size: 285 187 183 263
Fragments arranged by size:
285 263 187 183
BseYI C'CCAG_C
Cuts at: 0 137 798 875 918
Size: 137 661 77 43
Fragments arranged by size:
661 137 77 43
BsiHKAI G_wGCw'C
Cuts at: 0 37 246 304 482 536 918
Size: 37 209 58 178 54 382
Fragments arranged by size:
382 209 178 58 54 37
BsmI GAATG_Cn'
Cuts at: 0 903 918
Size: 903 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 918
Size: 160 758
Bsp1286I G_dGCh'C
Cuts at: 0 37 246 304 482 536 794 918
Size: 37 209 58 178 54 258 124
Fragments arranged by size:
258 209 178 124 58 54 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 517 855 918
Size: 461 56 338 63
Fragments arranged by size:
461 338 63 56
BsrFI r'CCGG_y
Cuts at: 0 904 918
Size: 904 14
BsrGI T'GTAC_A
Cuts at: 0 657 918
Size: 657 261
BssSI C'ACGA_G
Cuts at: 0 793 918
Size: 793 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 782 835 918
Size: 782 53 83
Fragments arranged by size:
782 83 53
BtgI C'CryG_G
Cuts at: 0 164 333 446 918
Size: 164 169 113 472
Fragments arranged by size:
472 169 164 113
DraIII CAC_nnn'GTG
Cuts at: 0 621 918
Size: 621 297
EaeI y'GGCC_r
Cuts at: 0 2 837 918
Size: 2 835 81
Fragments arranged by size:
835 81 2
EarI CTCTTCn'nnn_
Cuts at: 0 430 918
Size: 430 488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 601 909 918
Size: 601 308 9
Fragments arranged by size:
601 308 9
EcoICRI GAG'CTC
Cuts at: 0 35 480 918
Size: 35 445 438
Fragments arranged by size:
445 438 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 292 400 454 625 918
Size: 159 133 108 54 171 293
Fragments arranged by size:
293 171 159 133 108 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 918
Size: 310 608
EcoO109I rG'GnC_Cy
Cuts at: 0 233 396 506 918
Size: 233 163 110 412
Fragments arranged by size:
412 233 163 110
EcoRV GAT'ATC
Cuts at: 0 21 918
Size: 21 897
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 918
Size: 93 32 793
Fragments arranged by size:
793 93 32
HincII GTy'rAC
Cuts at: 0 522 918
Size: 522 396
HindIII A'AGCT_T
Cuts at: 0 278 918
Size: 278 640
MscI TGG'CCA
Cuts at: 0 4 918
Size: 4 914
MslI CAynn'nnrTG
Cuts at: 0 65 223 918
Size: 65 158 695
Fragments arranged by size:
695 158 65
MspA1I CmG'CkG
Cuts at: 0 30 249 545 603 842 875 918
Size: 30 219 296 58 239 33 43
Fragments arranged by size:
296 239 219 58 43 33 30
NcoI C'CATG_G
Cuts at: 0 164 918
Size: 164 754
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 918
Size: 144 774
PfoI T'CCnGG_A
Cuts at: 0 565 918
Size: 565 353
PpuMI rG'GwC_Cy
Cuts at: 0 233 396 506 918
Size: 233 163 110 412
Fragments arranged by size:
412 233 163 110
PshAI GACnn'nnGTC
Cuts at: 0 313 918
Size: 313 605
PspXI vC'TCGA_Gb
Cuts at: 0 297 918
Size: 297 621
PstI C_TGCA'G
Cuts at: 0 866 918
Size: 866 52
PvuII CAG'CTG
Cuts at: 0 30 249 545 603 918
Size: 30 219 296 58 315
Fragments arranged by size:
315 296 219 58 30
SacI G_AGCT'C
Cuts at: 0 37 482 918
Size: 37 445 436
Fragments arranged by size:
445 436 37
SanDI GG'GwC_CC
Cuts at: 0 233 918
Size: 233 685
SapI GCTCTTCn'nnn_
Cuts at: 0 430 918
Size: 430 488
SbfI CC_TGCA'GG
Cuts at: 0 866 918
Size: 866 52
ScaI AGT'ACT
Cuts at: 0 595 918
Size: 595 323
SexAI A'CCwGG_T
Cuts at: 0 617 918
Size: 617 301
SfcI C'TryA_G
Cuts at: 0 738 862 918
Size: 738 124 56
Fragments arranged by size:
738 124 56
SgrAI Cr'CCGG_yG
Cuts at: 0 904 918
Size: 904 14
SmlI C'TyrA_G
Cuts at: 0 297 918
Size: 297 621
StuI AGG'CCT
Cuts at: 0 574 918
Size: 574 344
StyI C'CwwG_G
Cuts at: 0 5 164 366 537 918
Size: 5 159 202 171 381
Fragments arranged by size:
381 202 171 159 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 346 516 711 790 867 918
Size: 337 9 170 195 79 77 51
Fragments arranged by size:
337 195 170 79 77 51 9
TatI w'GTAC_w
Cuts at: 0 593 657 918
Size: 593 64 261
Fragments arranged by size:
593 261 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 329 361 918
Size: 329 32 557
Fragments arranged by size:
557 329 32
Tth111I GACn'n_nGTC
Cuts at: 0 231 918
Size: 231 687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 918
Size: 909 9
XhoI C'TCGA_G
Cuts at: 0 297 918
Size: 297 621
XmnI GAAnn'nnTTC
Cuts at: 0 439 673 918
Size: 439 234 245
Fragments arranged by size:
439 245 234
Enzymes that do cut:
AarI AcuI AlwNI ApaLI ApoI AvaI AvrII BanII
BbsI BbvCI BlpI Bme1580I BmrI BpmI Bpu10I BsaI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrFI BsrGI BssSI BstXI BtgI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII
HindIII MscI MslI MspA1I NcoI PflMI PfoI PpuMI
PshAI PspXI PstI PvuII SacI SanDI SapI SbfI
ScaI SexAI SfcI SgrAI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiWI BspEI BspHI BsrBI
BsrDI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoRI
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII
SalI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XmaI ZraI