Restriction map for
pF1KB6430
(Linear) (Six-base) MAPSORT of: KIBB6430.seq Check: 3330 from: 1 to: 1029
KIBB6430.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AflIII A'CryG_T
Cuts at: 0 648 857 1029
Size: 648 209 172
Fragments arranged by size:
648 209 172
AhdI GACnn_n'nnGTC
Cuts at: 0 396 1029
Size: 396 633
AleI CACnn'nnGTG
Cuts at: 0 577 1029
Size: 577 452
AlwNI CAG_nnn'CTG
Cuts at: 0 327 1029
Size: 327 702
ApaI G_GGCC'C
Cuts at: 0 63 702 1029
Size: 63 639 327
Fragments arranged by size:
639 327 63
AvaI C'yCGr_G
Cuts at: 0 54 1029
Size: 54 975
BanI G'GyrC_C
Cuts at: 0 27 231 831 1029
Size: 27 204 600 198
Fragments arranged by size:
600 204 198 27
BanII G_rGCy'C
Cuts at: 0 63 91 146 702 776 893 1024 1029
Size: 63 28 55 556 74 117 131 5
Fragments arranged by size:
556 131 117 74 63 55 28 5
BbeI G_GCGC'C
Cuts at: 0 31 235 1029
Size: 31 204 794
Fragments arranged by size:
794 204 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 908 1029
Size: 10 898 121
Fragments arranged by size:
898 121 10
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1029
Size: 541 488
BglII A'GATC_T
Cuts at: 0 509 1029
Size: 509 520
Bme1580I G_kGCm'C
Cuts at: 0 63 574 702 1029
Size: 63 511 128 327
Fragments arranged by size:
511 327 128 63
BmgBI CAC'GTC
Cuts at: 0 768 1029
Size: 768 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 586 622 860 1004 1029
Size: 355 231 36 238 144 25
Fragments arranged by size:
355 238 231 144 36 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 70 775 1029
Size: 70 705 254
Fragments arranged by size:
705 254 70
BsaAI yAC'GTr
Cuts at: 0 168 575 651 860 1029
Size: 168 407 76 209 169
Fragments arranged by size:
407 209 169 168 76
BsaHI Gr'CG_yC
Cuts at: 0 7 28 232 408 1029
Size: 7 21 204 176 621
Fragments arranged by size:
621 204 176 21 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 463 493 559 589 977 1007
Size: 398 30 35 30 66 30 388 30
Cuts at: 1007 1029
Size: 22
Fragments arranged by size:
398 388 66 35 30 30 30 30
22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 403 859 1029
Size: 286 117 456 170
Fragments arranged by size:
456 286 170 117
BseYI C'CCAG_C
Cuts at: 0 695 1029
Size: 695 334
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1029
Size: 889 140
BsiEI CG_ry'CG
Cuts at: 0 135 995 1029
Size: 135 860 34
Fragments arranged by size:
860 135 34
BsiHKAI G_wGCw'C
Cuts at: 0 776 1024 1029
Size: 776 248 5
Fragments arranged by size:
776 248 5
Bsp1286I G_dGCh'C
Cuts at: 0 63 91 146 574 702 776 893 1024
Size: 63 28 55 428 128 74 117 131
Cuts at: 1024 1029
Size: 5
Fragments arranged by size:
428 131 128 117 74 63 55 28
5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 482 1029
Size: 326 156 547
Fragments arranged by size:
547 326 156
BssHII G'CGCG_C
Cuts at: 0 627 1029
Size: 627 402
BssSI C'ACGA_G
Cuts at: 0 775 1029
Size: 775 254
BstAPI GCAn_nnn'nTGC
Cuts at: 0 186 327 1029
Size: 186 141 702
Fragments arranged by size:
702 186 141
BstYI r'GATC_y
Cuts at: 0 509 1029
Size: 509 520
Bsu36I CC'TnA_GG
Cuts at: 0 688 1029
Size: 688 341
BtgI C'CryG_G
Cuts at: 0 135 158 226 235 417 1029
Size: 135 23 68 9 182 612
Fragments arranged by size:
612 182 135 68 23 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 138 1029
Size: 138 891
BtsI GCAGTG_nn'
Cuts at: 0 497 1029
Size: 497 532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 34 1029
Size: 34 995
EaeI y'GGCC_r
Cuts at: 0 132 533 1029
Size: 132 401 496
Fragments arranged by size:
496 401 132
EagI C'GGCC_G
Cuts at: 0 132 1029
Size: 132 897
EarI CTCTTCn'nnn_
Cuts at: 0 46 263 1012 1029
Size: 46 217 749 17
Fragments arranged by size:
749 217 46 17
EcoICRI GAG'CTC
Cuts at: 0 774 1022 1029
Size: 774 248 7
Fragments arranged by size:
774 248 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 586 622 860 1004 1029
Size: 355 231 36 238 144 25
Fragments arranged by size:
355 238 231 144 36 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 1029
Size: 466 563
EcoO109I rG'GnC_Cy
Cuts at: 0 59 60 471 699 1029
Size: 59 1 411 228 330
Fragments arranged by size:
411 330 228 59 1
HaeII r_GCGC'y
Cuts at: 0 31 116 235 1029
Size: 31 85 119 794
Fragments arranged by size:
794 119 85 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 382 398 414 430 463 495
Size: 187 32 163 16 16 16 33 32
Cuts at: 495 977 1009 1029
Size: 482 32 20
Fragments arranged by size:
482 187 163 33 32 32 32 20
16 16 16
KasI G'GCGC_C
Cuts at: 0 27 231 1029
Size: 27 204 798
Fragments arranged by size:
798 204 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 1029
Size: 425 604
MslI CAynn'nnrTG
Cuts at: 0 157 577 1029
Size: 157 420 452
Fragments arranged by size:
452 420 157
MspA1I CmG'CkG
Cuts at: 0 137 228 419 493 741 823 973 1029
Size: 137 91 191 74 248 82 150 56
Fragments arranged by size:
248 191 150 137 91 82 74 56
NarI GG'CG_CC
Cuts at: 0 28 232 1029
Size: 28 204 797
Fragments arranged by size:
797 204 28
NcoI C'CATG_G
Cuts at: 0 158 1029
Size: 158 871
PasI CC'CwG_GG
Cuts at: 0 702 1029
Size: 702 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1029
Size: 768 261
PfoI T'CCnGG_A
Cuts at: 0 481 1029
Size: 481 548
PmlI CAC'GTG
Cuts at: 0 575 651 860 1029
Size: 575 76 209 169
Fragments arranged by size:
575 209 169 76
PpuMI rG'GwC_Cy
Cuts at: 0 471 1029
Size: 471 558
PspOMI G'GGCC_C
Cuts at: 0 59 698 1029
Size: 59 639 331
Fragments arranged by size:
639 331 59
PstI C_TGCA'G
Cuts at: 0 323 626 795 1029
Size: 323 303 169 234
Fragments arranged by size:
323 303 234 169
PvuII CAG'CTG
Cuts at: 0 823 973 1029
Size: 823 150 56
Fragments arranged by size:
823 150 56
SacI G_AGCT'C
Cuts at: 0 776 1024 1029
Size: 776 248 5
Fragments arranged by size:
776 248 5
SacII CC_GC'GG
Cuts at: 0 138 229 420 1029
Size: 138 91 191 609
Fragments arranged by size:
609 191 138 91
SapI GCTCTTCn'nnn_
Cuts at: 0 1012 1029
Size: 1012 17
SbfI CC_TGCA'GG
Cuts at: 0 795 1029
Size: 795 234
SfcI C'TryA_G
Cuts at: 0 319 622 791 974 1029
Size: 319 303 169 183 55
Fragments arranged by size:
319 303 183 169 55
SfoI GGC'GCC
Cuts at: 0 29 233 1029
Size: 29 204 796
Fragments arranged by size:
796 204 29
StuI AGG'CCT
Cuts at: 0 789 1029
Size: 789 240
StyI C'CwwG_G
Cuts at: 0 10 158 287 1029
Size: 10 148 129 742
Fragments arranged by size:
742 148 129 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 228 1029
Size: 88 140 801
Fragments arranged by size:
801 140 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 1029
Size: 559 32 438
Fragments arranged by size:
559 438 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 379 936 1029
Size: 165 214 557 93
Fragments arranged by size:
557 214 165 93
Enzymes that do cut:
AflIII AhdI AleI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BglII Bme1580I BmgBI BpmI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BssHII BssSI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI CspCI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I
NarI NcoI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SapI SbfI SfcI SfoI
StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BstBI BstEII BstXI BstZ17I ClaI DraI DraIII
DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI