Restriction map for
pF1KB6431
(Linear) (Six-base) MAPSORT of: KIBB6431.seq Check: 4145 from: 1 to: 936
KIBB6431.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
Acc65I G'GTAC_C
Cuts at: 0 386 936
Size: 386 550
AleI CACnn'nnGTG
Cuts at: 0 599 721 936
Size: 599 122 215
Fragments arranged by size:
599 215 122
AlwNI CAG_nnn'CTG
Cuts at: 0 189 396 459 619 936
Size: 189 207 63 160 317
Fragments arranged by size:
317 207 189 160 63
ApaI G_GGCC'C
Cuts at: 0 656 936
Size: 656 280
ApaLI G'TGCA_C
Cuts at: 0 724 877 936
Size: 724 153 59
Fragments arranged by size:
724 153 59
AvaI C'yCGr_G
Cuts at: 0 73 106 936
Size: 73 33 830
Fragments arranged by size:
830 73 33
AvrII C'CTAG_G
Cuts at: 0 811 936
Size: 811 125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 370 403 936
Size: 370 33 533
Fragments arranged by size:
533 370 33
BanI G'GyrC_C
Cuts at: 0 249 386 445 936
Size: 249 137 59 491
Fragments arranged by size:
491 249 137 59
BanII G_rGCy'C
Cuts at: 0 107 211 242 266 656 936
Size: 107 104 31 24 390 280
Fragments arranged by size:
390 280 107 104 31 24
BbeI G_GCGC'C
Cuts at: 0 449 936
Size: 449 487
BbvCI CC'TCA_GC
Cuts at: 0 745 936
Size: 745 191
BglI GCCn_nnn'nGGC
Cuts at: 0 622 936
Size: 622 314
BlpI GC'TnA_GC
Cuts at: 0 236 936
Size: 236 700
Bme1580I G_kGCm'C
Cuts at: 0 369 656 694 728 881 936
Size: 369 287 38 34 153 55
Fragments arranged by size:
369 287 153 55 38 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 399 564 817 936
Size: 345 54 165 253 119
Fragments arranged by size:
345 253 165 119 54
Bpu10I CC'TnA_GC
Cuts at: 0 745 936
Size: 745 191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 229 936
Size: 54 175 707
Fragments arranged by size:
707 175 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 936
Size: 775 161
BsaHI Gr'CG_yC
Cuts at: 0 446 936
Size: 446 490
BsaWI w'CCGG_w
Cuts at: 0 116 936
Size: 116 820
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 561 793 814 936
Size: 115 446 232 21 122
Fragments arranged by size:
446 232 122 115 21
BseYI C'CCAG_C
Cuts at: 0 79 513 612 936
Size: 79 434 99 324
Fragments arranged by size:
434 324 99 79
BsiEI CG_ry'CG
Cuts at: 0 923 936
Size: 923 13
BsiHKAI G_wGCw'C
Cuts at: 0 350 728 881 936
Size: 350 378 153 55
Fragments arranged by size:
378 350 153 55
Bsp1286I G_dGCh'C
Cuts at: 0 107 211 242 266 350 369 656 694
Size: 107 104 31 24 84 19 287 38
Cuts at: 694 728 881 936
Size: 34 153 55
Fragments arranged by size:
287 153 107 104 84 55 38 34
31 24 19
BspEI T'CCGG_A
Cuts at: 0 116 936
Size: 116 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 936
Size: 374 562
BsrDI GCAATG_nn'
Cuts at: 0 704 936
Size: 704 232
BssSI C'ACGA_G
Cuts at: 0 826 936
Size: 826 110
BstAPI GCAn_nnn'nTGC
Cuts at: 0 459 936
Size: 459 477
BtgI C'CryG_G
Cuts at: 0 85 172 936
Size: 85 87 764
Fragments arranged by size:
764 87 85
BtsI GCAGTG_nn'
Cuts at: 0 348 716 936
Size: 348 368 220
Fragments arranged by size:
368 348 220
EaeI y'GGCC_r
Cuts at: 0 623 855 920 936
Size: 623 232 65 16
Fragments arranged by size:
623 232 65 16
EagI C'GGCC_G
Cuts at: 0 920 936
Size: 920 16
EarI CTCTTCn'nnn_
Cuts at: 0 117 936
Size: 117 819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 399 564 817 936
Size: 345 54 165 253 119
Fragments arranged by size:
345 253 165 119 54
EcoO109I rG'GnC_Cy
Cuts at: 0 70 471 936
Size: 70 401 465
Fragments arranged by size:
465 401 70
HaeII r_GCGC'y
Cuts at: 0 449 581 640 936
Size: 449 132 59 296
Fragments arranged by size:
449 296 132 59
HincII GTy'rAC
Cuts at: 0 291 936
Size: 291 645
KasI G'GCGC_C
Cuts at: 0 445 936
Size: 445 491
KpnI G_GTAC'C
Cuts at: 0 390 936
Size: 390 546
MslI CAynn'nnrTG
Cuts at: 0 353 599 709 721 936
Size: 353 246 110 12 215
Fragments arranged by size:
353 246 215 110 12
MspA1I CmG'CkG
Cuts at: 0 87 174 285 936
Size: 87 87 111 651
Fragments arranged by size:
651 111 87 87
NarI GG'CG_CC
Cuts at: 0 446 936
Size: 446 490
NspI r_CATG'y
Cuts at: 0 724 936
Size: 724 212
PasI CC'CwG_GG
Cuts at: 0 927 936
Size: 927 9
PpuMI rG'GwC_Cy
Cuts at: 0 70 936
Size: 70 866
PspOMI G'GGCC_C
Cuts at: 0 652 936
Size: 652 284
PstI C_TGCA'G
Cuts at: 0 135 497 590 936
Size: 135 362 93 346
Fragments arranged by size:
362 346 135 93
PvuII CAG'CTG
Cuts at: 0 285 936
Size: 285 651
SacII CC_GC'GG
Cuts at: 0 88 175 936
Size: 88 87 761
Fragments arranged by size:
761 88 87
SanDI GG'GwC_CC
Cuts at: 0 70 936
Size: 70 866
SexAI A'CCwGG_T
Cuts at: 0 389 431 936
Size: 389 42 505
Fragments arranged by size:
505 389 42
SfcI C'TryA_G
Cuts at: 0 131 493 586 936
Size: 131 362 93 350
Fragments arranged by size:
362 350 131 93
SfoI GGC'GCC
Cuts at: 0 447 936
Size: 447 489
SmaI CCC'GGG
Cuts at: 0 75 108 936
Size: 75 33 828
Fragments arranged by size:
828 75 33
SmlI C'TyrA_G
Cuts at: 0 33 244 936
Size: 33 211 692
Fragments arranged by size:
692 211 33
SrfI GCCC'GGGC
Cuts at: 0 108 936
Size: 108 828
StyI C'CwwG_G
Cuts at: 0 811 936
Size: 811 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 936
Size: 830 32 74
Fragments arranged by size:
830 74 32
XmaI C'CCGG_G
Cuts at: 0 73 106 936
Size: 73 33 830
Fragments arranged by size:
830 73 33
Enzymes that do cut:
Acc65I AleI AlwNI ApaI ApaLI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BglI BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrDI BssSI BstAPI BtgI
BtsI EaeI EagI EarI Eco57MI EcoO109I HaeII HincII
KasI KpnI MslI MspA1I NarI NspI PasI PpuMI
PspOMI PstI PvuII SacII SanDI SexAI SfcI SfoI
SmaI SmlI SrfI StyI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaXI BsgI BsiWI
BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI MfeI
MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SapI SbfI ScaI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmnI ZraI