Restriction map for
pF1KB6436
(Linear) (Six-base) MAPSORT of: KIBB6436.seq Check: 586 from: 1 to: 933
KIBB6436.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 933
Size: 823 110
AflII C'TTAA_G
Cuts at: 0 283 933
Size: 283 650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 378 431 933
Size: 378 53 502
Fragments arranged by size:
502 378 53
ApaLI G'TGCA_C
Cuts at: 0 416 933
Size: 416 517
ApoI r'AATT_y
Cuts at: 0 472 617 769 898 933
Size: 472 145 152 129 35
Fragments arranged by size:
472 152 145 129 35
BanI G'GyrC_C
Cuts at: 0 48 751 933
Size: 48 703 182
Fragments arranged by size:
703 182 48
BbeI G_GCGC'C
Cuts at: 0 52 933
Size: 52 881
BbsI GAAGACnn'nnnn_
Cuts at: 0 843 933
Size: 843 90
BbvCI CC'TCA_GC
Cuts at: 0 785 933
Size: 785 148
BglI GCCn_nnn'nGGC
Cuts at: 0 13 46 57 933
Size: 13 33 11 876
Fragments arranged by size:
876 33 13 11
Bme1580I G_kGCm'C
Cuts at: 0 420 933
Size: 420 513
BmrI ACTGGGnnnn_n'
Cuts at: 0 826 843 933
Size: 826 17 90
Fragments arranged by size:
826 90 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 324 411 933
Size: 262 62 87 522
Fragments arranged by size:
522 262 87 62
Bpu10I CC'TnA_GC
Cuts at: 0 785 933
Size: 785 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 704 933
Size: 589 115 229
Fragments arranged by size:
589 229 115
BsaHI Gr'CG_yC
Cuts at: 0 49 119 933
Size: 49 70 814
Fragments arranged by size:
814 70 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 254 284 543 573 933
Size: 254 30 259 30 360
Fragments arranged by size:
360 259 254 30 30
BseYI C'CCAG_C
Cuts at: 0 30 82 933
Size: 30 52 851
Fragments arranged by size:
851 52 30
BsiHKAI G_wGCw'C
Cuts at: 0 297 340 420 933
Size: 297 43 80 513
Fragments arranged by size:
513 297 80 43
Bsp1286I G_dGCh'C
Cuts at: 0 297 340 420 933
Size: 297 43 80 513
Fragments arranged by size:
513 297 80 43
BsrFI r'CCGG_y
Cuts at: 0 51 172 933
Size: 51 121 761
Fragments arranged by size:
761 121 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 24 933
Size: 24 909
Bsu36I CC'TnA_GG
Cuts at: 0 512 933
Size: 512 421
BtgI C'CryG_G
Cuts at: 0 587 933
Size: 587 346
DraI TTT'AAA
Cuts at: 0 633 933
Size: 633 300
EaeI y'GGCC_r
Cuts at: 0 108 933
Size: 108 825
EarI CTCTTCn'nnn_
Cuts at: 0 148 933
Size: 148 785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 324 411 823 933
Size: 262 62 87 412 110
Fragments arranged by size:
412 262 110 87 62
EcoO109I rG'GnC_Cy
Cuts at: 0 325 933
Size: 325 608
EcoRI G'AATT_C
Cuts at: 0 769 933
Size: 769 164
FspI TGC'GCA
Cuts at: 0 67 933
Size: 67 866
HaeII r_GCGC'y
Cuts at: 0 52 933
Size: 52 881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 933
Size: 205 32 696
Fragments arranged by size:
696 205 32
KasI G'GCGC_C
Cuts at: 0 48 933
Size: 48 885
MspA1I CmG'CkG
Cuts at: 0 102 131 933
Size: 102 29 802
Fragments arranged by size:
802 102 29
NaeI GCC'GGC
Cuts at: 0 53 174 933
Size: 53 121 759
Fragments arranged by size:
759 121 53
NarI GG'CG_CC
Cuts at: 0 49 933
Size: 49 884
NcoI C'CATG_G
Cuts at: 0 587 933
Size: 587 346
NdeI CA'TA_TG
Cuts at: 0 225 933
Size: 225 708
NgoMIV G'CCGG_C
Cuts at: 0 51 172 933
Size: 51 121 761
Fragments arranged by size:
761 121 51
PstI C_TGCA'G
Cuts at: 0 307 367 874 933
Size: 307 60 507 59
Fragments arranged by size:
507 307 60 59
SfcI C'TryA_G
Cuts at: 0 303 363 870 933
Size: 303 60 507 63
Fragments arranged by size:
507 303 63 60
SfoI GGC'GCC
Cuts at: 0 50 933
Size: 50 883
SgrAI Cr'CCGG_yG
Cuts at: 0 51 933
Size: 51 882
SmlI C'TyrA_G
Cuts at: 0 283 568 719 933
Size: 283 285 151 214
Fragments arranged by size:
285 283 214 151
SspI AAT'ATT
Cuts at: 0 714 764 933
Size: 714 50 169
Fragments arranged by size:
714 169 50
StuI AGG'CCT
Cuts at: 0 21 933
Size: 21 912
StyI C'CwwG_G
Cuts at: 0 587 933
Size: 587 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 933
Size: 547 386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 543 575 690 722 933
Size: 254 32 257 32 115 32 211
Fragments arranged by size:
257 254 211 115 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 286 762 933
Size: 286 476 171
Fragments arranged by size:
476 286 171
XmnI GAAnn'nnTTC
Cuts at: 0 662 713 933
Size: 662 51 220
Fragments arranged by size:
662 220 51
Enzymes that do cut:
AcuI AflII AlfI ApaLI ApoI BanI BbeI BbsI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI
BsaXI BseYI BsiHKAI Bsp1286I BsrFI BstXI Bsu36I BtgI
DraI EaeI EarI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I KasI MspA1I NaeI NarI NcoI NdeI NgoMIV
PstI SfcI SfoI SgrAI SmlI SspI StuI StyI
TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI
SrfI SwaI TatI Tth111I XbaI XhoI XmaI ZraI