Restriction map for
pF1KB6437
(Linear) (Six-base) MAPSORT of: KIBB6437.seq Check: 5911 from: 1 to: 939
KIBB6437.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 341 939
Size: 341 598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 939
Size: 589 350
ApaLI G'TGCA_C
Cuts at: 0 650 939
Size: 650 289
ApoI r'AATT_y
Cuts at: 0 114 157 323 444 939
Size: 114 43 166 121 495
Fragments arranged by size:
495 166 121 114 43
AvrII C'CTAG_G
Cuts at: 0 870 939
Size: 870 69
BanII G_rGCy'C
Cuts at: 0 33 939
Size: 33 906
BglI GCCn_nnn'nGGC
Cuts at: 0 74 939
Size: 74 865
Bme1580I G_kGCm'C
Cuts at: 0 654 939
Size: 654 285
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 502 939
Size: 502 437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 939
Size: 283 656
Bpu10I CC'TnA_GC
Cuts at: 0 134 473 939
Size: 134 339 466
Fragments arranged by size:
466 339 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 426 939
Size: 426 513
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 704 734 939
Size: 704 30 205
Fragments arranged by size:
704 205 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 542 939
Size: 542 397
BseYI C'CCAG_C
Cuts at: 0 32 55 939
Size: 32 23 884
Fragments arranged by size:
884 32 23
BsiHKAI G_wGCw'C
Cuts at: 0 654 939
Size: 654 285
BsmI GAATG_Cn'
Cuts at: 0 298 939
Size: 298 641
Bsp1286I G_dGCh'C
Cuts at: 0 33 654 939
Size: 33 621 285
Fragments arranged by size:
621 285 33
BsrGI T'GTAC_A
Cuts at: 0 765 939
Size: 765 174
BstYI r'GATC_y
Cuts at: 0 855 939
Size: 855 84
BtgI C'CryG_G
Cuts at: 0 146 939
Size: 146 793
DraI TTT'AAA
Cuts at: 0 285 939
Size: 285 654
EarI CTCTTCn'nnn_
Cuts at: 0 41 939
Size: 41 898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 589 939
Size: 283 306 350
Fragments arranged by size:
350 306 283
EcoRI G'AATT_C
Cuts at: 0 323 939
Size: 323 616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 816 939
Size: 816 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 474 506 939
Size: 256 32 186 32 433
Fragments arranged by size:
433 256 186 32 32
HincII GTy'rAC
Cuts at: 0 252 386 939
Size: 252 134 553
Fragments arranged by size:
553 252 134
HpaI GTT'AAC
Cuts at: 0 252 939
Size: 252 687
MfeI C'AATT_G
Cuts at: 0 824 939
Size: 824 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 939
Size: 844 95
MslI CAynn'nnrTG
Cuts at: 0 151 533 939
Size: 151 382 406
Fragments arranged by size:
406 382 151
NcoI C'CATG_G
Cuts at: 0 146 939
Size: 146 793
NspI r_CATG'y
Cuts at: 0 81 340 939
Size: 81 259 599
Fragments arranged by size:
599 259 81
PfoI T'CCnGG_A
Cuts at: 0 318 939
Size: 318 621
PstI C_TGCA'G
Cuts at: 0 570 939
Size: 570 369
SfcI C'TryA_G
Cuts at: 0 566 666 863 876 939
Size: 566 100 197 13 63
Fragments arranged by size:
566 197 100 63 13
SphI G_CATG'C
Cuts at: 0 81 939
Size: 81 858
SspI AAT'ATT
Cuts at: 0 237 939
Size: 237 702
StyI C'CwwG_G
Cuts at: 0 146 870 939
Size: 146 724 69
Fragments arranged by size:
724 146 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 387 939
Size: 387 552
TatI w'GTAC_w
Cuts at: 0 765 939
Size: 765 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 939
Size: 764 32 143
Fragments arranged by size:
764 143 32
Enzymes that do cut:
AccI AcuI ApaLI ApoI AvrII BanII BglI Bme1580I
BplI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BsrGI BstYI BtgI DraI EarI Eco57MI
EcoRI FalI Hin4I HincII HpaI MfeI MmeI MslI
NcoI NspI PfoI PstI SfcI SphI SspI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HindIII KasI KpnI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI