Restriction map for
pF1KB6442
(Linear) (Six-base) MAPSORT of: KIBB6442.seq Check: 9452 from: 1 to: 1038
KIBB6442.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 434 1038
Size: 434 604
AatII G_ACGT'C
Cuts at: 0 865 1038
Size: 865 173
Acc65I G'GTAC_C
Cuts at: 0 608 1038
Size: 608 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 142 313 1038
Size: 87 55 171 725
Fragments arranged by size:
725 171 87 55
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 1038
Size: 61 32 945
Fragments arranged by size:
945 61 32
AlwNI CAG_nnn'CTG
Cuts at: 0 638 1033 1038
Size: 638 395 5
Fragments arranged by size:
638 395 5
AvaI C'yCGr_G
Cuts at: 0 886 1038
Size: 886 152
AvrII C'CTAG_G
Cuts at: 0 108 1038
Size: 108 930
BanI G'GyrC_C
Cuts at: 0 608 1038
Size: 608 430
BanII G_rGCy'C
Cuts at: 0 59 373 401 728 1038
Size: 59 314 28 327 310
Fragments arranged by size:
327 314 310 59 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 210 466 1038
Size: 210 256 572
Fragments arranged by size:
572 256 210
BglI GCCn_nnn'nGGC
Cuts at: 0 1030 1038
Size: 1030 8
BglII A'GATC_T
Cuts at: 0 239 1038
Size: 239 799
BlpI GC'TnA_GC
Cuts at: 0 763 1038
Size: 763 275
Bme1580I G_kGCm'C
Cuts at: 0 182 1038
Size: 182 856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 943 979 1038
Size: 397 546 36 59
Fragments arranged by size:
546 397 59 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1038
Size: 385 653
BsaI GGTCTCn'nnnn_
Cuts at: 0 934 1038
Size: 934 104
BsaHI Gr'CG_yC
Cuts at: 0 251 623 862 1038
Size: 251 372 239 176
Fragments arranged by size:
372 251 239 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 547 577 1038
Size: 61 30 456 30 461
Fragments arranged by size:
461 456 61 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 736 799 1038
Size: 156 580 63 239
Fragments arranged by size:
580 239 156 63
BseYI C'CCAG_C
Cuts at: 0 232 261 975 1038
Size: 232 29 714 63
Fragments arranged by size:
714 232 63 29
BsiHKAI G_wGCw'C
Cuts at: 0 728 1038
Size: 728 310
Bsp1286I G_dGCh'C
Cuts at: 0 59 182 373 401 728 1038
Size: 59 123 191 28 327 310
Fragments arranged by size:
327 310 191 123 59 28
BspHI T'CATG_A
Cuts at: 0 65 452 782 1038
Size: 65 387 330 256
Fragments arranged by size:
387 330 256 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 434 1038
Size: 434 604
BstEII G'GTnAC_C
Cuts at: 0 828 1038
Size: 828 210
BstYI r'GATC_y
Cuts at: 0 239 1038
Size: 239 799
BtgI C'CryG_G
Cuts at: 0 655 791 1038
Size: 655 136 247
Fragments arranged by size:
655 247 136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 161 1038
Size: 161 877
EarI CTCTTCn'nnn_
Cuts at: 0 325 340 826 922 1038
Size: 325 15 486 96 116
Fragments arranged by size:
486 325 116 96 15
EcoICRI GAG'CTC
Cuts at: 0 726 1038
Size: 726 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 142 313 397 943 979 1038
Size: 87 55 171 84 546 36 59
Fragments arranged by size:
546 171 87 84 59 55 36
EcoO109I rG'GnC_Cy
Cuts at: 0 1023 1038
Size: 1023 15
HaeII r_GCGC'y
Cuts at: 0 7 1038
Size: 7 1031
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 528 547 560 579 862 894 1038
Size: 528 19 13 19 283 32 144
Fragments arranged by size:
528 283 144 32 19 19 13
KpnI G_GTAC'C
Cuts at: 0 612 1038
Size: 612 426
MfeI C'AATT_G
Cuts at: 0 839 1038
Size: 839 199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1038
Size: 905 133
MslI CAynn'nnrTG
Cuts at: 0 469 1038
Size: 469 569
MspA1I CmG'CkG
Cuts at: 0 635 1038
Size: 635 403
NcoI C'CATG_G
Cuts at: 0 791 1038
Size: 791 247
NspI r_CATG'y
Cuts at: 0 474 1038
Size: 474 564
PasI CC'CwG_GG
Cuts at: 0 387 1038
Size: 387 651
PflMI CCAn_nnn'nTGG
Cuts at: 0 1033 1038
Size: 1033 5
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 1038
Size: 61 32 945
Fragments arranged by size:
945 61 32
PstI C_TGCA'G
Cuts at: 0 479 751 1038
Size: 479 272 287
Fragments arranged by size:
479 287 272
PvuII CAG'CTG
Cuts at: 0 635 1038
Size: 635 403
SacI G_AGCT'C
Cuts at: 0 728 1038
Size: 728 310
SapI GCTCTTCn'nnn_
Cuts at: 0 826 922 1038
Size: 826 96 116
Fragments arranged by size:
826 116 96
ScaI AGT'ACT
Cuts at: 0 418 1038
Size: 418 620
SexAI A'CCwGG_T
Cuts at: 0 611 989 1038
Size: 611 378 49
Fragments arranged by size:
611 378 49
SfcI C'TryA_G
Cuts at: 0 475 699 747 771 856 1038
Size: 475 224 48 24 85 182
Fragments arranged by size:
475 224 182 85 48 24
SmlI C'TyrA_G
Cuts at: 0 400 1038
Size: 400 638
StuI AGG'CCT
Cuts at: 0 952 1038
Size: 952 86
StyI C'CwwG_G
Cuts at: 0 51 108 393 791 1038
Size: 51 57 285 398 247
Fragments arranged by size:
398 285 247 57 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 726 784 1038
Size: 726 58 254
Fragments arranged by size:
726 254 58
TatI w'GTAC_w
Cuts at: 0 416 1038
Size: 416 622
Tth111I GACn'n_nGTC
Cuts at: 0 622 1038
Size: 622 416
XmnI GAAnn'nnTTC
Cuts at: 0 73 1038
Size: 73 965
ZraI GAC'GTC
Cuts at: 0 863 1038
Size: 863 175
Enzymes that do cut:
AarI AatII Acc65I AcuI AloI AlwNI AvaI AvrII
BanI BanII BbsI BglI BglII BlpI Bme1580I BpmI
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspHI BspMI BstEII BstYI BtgI BtgZI EarI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KpnI MfeI MmeI MslI
MspA1I NcoI NspI PasI PflMI PpiI PstI PvuII
SacI SapI ScaI SexAI SfcI SmlI StuI StyI
TaqII TatI Tth111I XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XcmI XhoI XmaI