Restriction map for
pF1KB6445
(Linear) (Six-base) MAPSORT of: KIBB6445.seq Check: 7298 from: 1 to: 945
KIBB6445.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 564 945
Size: 282 282 381
Fragments arranged by size:
381 282 282
AccI GT'mk_AC
Cuts at: 0 302 650 902 945
Size: 302 348 252 43
Fragments arranged by size:
348 302 252 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 945
Size: 130 815
AflIII A'CryG_T
Cuts at: 0 551 945
Size: 551 394
AhdI GACnn_n'nnGTC
Cuts at: 0 507 727 945
Size: 507 220 218
Fragments arranged by size:
507 220 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 270 945
Size: 61 209 675
Fragments arranged by size:
675 209 61
ApaI G_GGCC'C
Cuts at: 0 448 945
Size: 448 497
ApoI r'AATT_y
Cuts at: 0 31 526 945
Size: 31 495 419
Fragments arranged by size:
495 419 31
AvaI C'yCGr_G
Cuts at: 0 313 333 357 669 945
Size: 313 20 24 312 276
Fragments arranged by size:
313 312 276 24 20
BanI G'GyrC_C
Cuts at: 0 282 856 945
Size: 282 574 89
Fragments arranged by size:
574 282 89
BanII G_rGCy'C
Cuts at: 0 85 437 448 761 889 896 945
Size: 85 352 11 313 128 7 49
Fragments arranged by size:
352 313 128 85 49 11 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 945
Size: 506 439
BbvCI CC'TCA_GC
Cuts at: 0 811 945
Size: 811 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 681 715 945
Size: 681 34 230
Fragments arranged by size:
681 230 34
BglI GCCn_nnn'nGGC
Cuts at: 0 15 945
Size: 15 930
BlpI GC'TnA_GC
Cuts at: 0 19 945
Size: 19 926
Bme1580I G_kGCm'C
Cuts at: 0 278 285 448 945
Size: 278 7 163 497
Fragments arranged by size:
497 278 163 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 945
Size: 288 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 945
Size: 99 846
Bpu10I CC'TnA_GC
Cuts at: 0 811 945
Size: 811 134
BsaHI Gr'CG_yC
Cuts at: 0 415 945
Size: 415 530
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 945
Size: 748 30 167
Fragments arranged by size:
748 167 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 445 945
Size: 298 147 500
Fragments arranged by size:
500 298 147
BseYI C'CCAG_C
Cuts at: 0 441 945
Size: 441 504
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 945
Size: 72 873
BsiHKAI G_wGCw'C
Cuts at: 0 85 631 707 945
Size: 85 546 76 238
Fragments arranged by size:
546 238 85 76
Bsp1286I G_dGCh'C
Cuts at: 0 85 278 285 437 448 631 707 761
Size: 85 193 7 152 11 183 76 54
Cuts at: 761 889 896 945
Size: 128 7 49
Fragments arranged by size:
193 183 152 128 85 76 54 49
11 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 564 611 662 686 945
Size: 282 282 47 51 24 259
Fragments arranged by size:
282 282 259 51 47 24
BsrBI CCG'CTC
Cuts at: 0 74 945
Size: 74 871
BsrDI GCAATG_nn'
Cuts at: 0 791 945
Size: 791 154
BsrFI r'CCGG_y
Cuts at: 0 914 945
Size: 914 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 945
Size: 828 117
Bsu36I CC'TnA_GG
Cuts at: 0 881 945
Size: 881 64
BtgI C'CryG_G
Cuts at: 0 843 945
Size: 843 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 377 945
Size: 377 568
BtsI GCAGTG_nn'
Cuts at: 0 231 402 945
Size: 231 171 543
Fragments arranged by size:
543 231 171
EcoICRI GAG'CTC
Cuts at: 0 83 945
Size: 83 862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 130 945
Size: 99 31 815
Fragments arranged by size:
815 99 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 96 945
Size: 96 849
EcoO109I rG'GnC_Cy
Cuts at: 0 143 807 945
Size: 143 664 138
Fragments arranged by size:
664 143 138
EcoRI G'AATT_C
Cuts at: 0 31 526 945
Size: 31 495 419
Fragments arranged by size:
495 419 31
EcoRV GAT'ATC
Cuts at: 0 933 945
Size: 933 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 713 745 945
Size: 493 32 188 32 200
Fragments arranged by size:
493 200 188 32 32
MfeI C'AATT_G
Cuts at: 0 533 945
Size: 533 412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 945
Size: 659 286
MslI CAynn'nnrTG
Cuts at: 0 556 826 945
Size: 556 270 119
Fragments arranged by size:
556 270 119
MspA1I CmG'CkG
Cuts at: 0 260 845 945
Size: 260 585 100
Fragments arranged by size:
585 260 100
PasI CC'CwG_GG
Cuts at: 0 513 945
Size: 513 432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 945
Size: 679 32 234
Fragments arranged by size:
679 234 32
PpuMI rG'GwC_Cy
Cuts at: 0 143 945
Size: 143 802
PshAI GACnn'nnGTC
Cuts at: 0 592 945
Size: 592 353
PspOMI G'GGCC_C
Cuts at: 0 444 945
Size: 444 501
PstI C_TGCA'G
Cuts at: 0 683 945
Size: 683 262
SacI G_AGCT'C
Cuts at: 0 85 945
Size: 85 860
SacII CC_GC'GG
Cuts at: 0 846 945
Size: 846 99
ScaI AGT'ACT
Cuts at: 0 159 945
Size: 159 786
SexAI A'CCwGG_T
Cuts at: 0 503 945
Size: 503 442
SfcI C'TryA_G
Cuts at: 0 679 945
Size: 679 266
SmaI CCC'GGG
Cuts at: 0 315 359 945
Size: 315 44 586
Fragments arranged by size:
586 315 44
SmlI C'TyrA_G
Cuts at: 0 333 945
Size: 333 612
StuI AGG'CCT
Cuts at: 0 182 945
Size: 182 763
StyI C'CwwG_G
Cuts at: 0 896 945
Size: 896 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 77 286 945
Size: 43 34 209 659
Fragments arranged by size:
659 209 43 34
TatI w'GTAC_w
Cuts at: 0 157 833 945
Size: 157 676 112
Fragments arranged by size:
676 157 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 748 780 849 881 945
Size: 748 32 69 32 64
Fragments arranged by size:
748 69 64 32 32
XhoI C'TCGA_G
Cuts at: 0 333 945
Size: 333 612
XmaI C'CCGG_G
Cuts at: 0 313 357 945
Size: 313 44 588
Fragments arranged by size:
588 313 44
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstXI
Bsu36I BtgI BtgZI BtsI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV Hin4I MfeI MmeI MslI MspA1I PasI
PpiI PpuMI PshAI PspOMI PstI SacI SacII ScaI
SexAI SfcI SmaI SmlI StuI StyI TaqII TatI
TstI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BclI BfrBI BglII BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XmnI
ZraI