Restriction map for
pF1KB6449
(Linear) (Six-base) MAPSORT of: KIBB6449.seq Check: 4486 from: 1 to: 951
KIBB6449.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 558 951
Size: 558 393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 547 814 951
Size: 79 468 267 137
Fragments arranged by size:
468 267 137 79
AflIII A'CryG_T
Cuts at: 0 551 553 951
Size: 551 2 398
Fragments arranged by size:
551 398 2
AhdI GACnn_n'nnGTC
Cuts at: 0 696 873 951
Size: 696 177 78
Fragments arranged by size:
696 177 78
AlwNI CAG_nnn'CTG
Cuts at: 0 804 837 942 951
Size: 804 33 105 9
Fragments arranged by size:
804 105 33 9
ApaI G_GGCC'C
Cuts at: 0 46 951
Size: 46 905
AvaI C'yCGr_G
Cuts at: 0 160 951
Size: 160 791
BanI G'GyrC_C
Cuts at: 0 174 951
Size: 174 777
BanII G_rGCy'C
Cuts at: 0 46 194 416 641 951
Size: 46 148 222 225 310
Fragments arranged by size:
310 225 222 148 46
BbvCI CC'TCA_GC
Cuts at: 0 380 951
Size: 380 571
BciVI GTATCCnnnnn_n'
Cuts at: 0 785 951
Size: 785 166
BclI T'GATC_A
Cuts at: 0 356 951
Size: 356 595
Bme1580I G_kGCm'C
Cuts at: 0 46 951
Size: 46 905
Bpu10I CC'TnA_GC
Cuts at: 0 380 410 632 951
Size: 380 30 222 319
Fragments arranged by size:
380 319 222 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 290 951
Size: 290 661
BsaAI yAC'GTr
Cuts at: 0 554 951
Size: 554 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 951
Size: 502 30 419
Fragments arranged by size:
502 419 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 871 951
Size: 133 738 80
Fragments arranged by size:
738 133 80
BseYI C'CCAG_C
Cuts at: 0 804 951
Size: 804 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 483 951
Size: 217 266 468
Fragments arranged by size:
468 266 217
BsiEI CG_ry'CG
Cuts at: 0 14 84 951
Size: 14 70 867
Fragments arranged by size:
867 70 14
BsiHKAI G_wGCw'C
Cuts at: 0 416 951
Size: 416 535
Bsp1286I G_dGCh'C
Cuts at: 0 46 194 416 641 951
Size: 46 148 222 225 310
Fragments arranged by size:
310 225 222 148 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 686 951
Size: 335 351 265
Fragments arranged by size:
351 335 265
BsrBI CCG'CTC
Cuts at: 0 69 951
Size: 69 882
BstAPI GCAn_nnn'nTGC
Cuts at: 0 174 951
Size: 174 777
BstYI r'GATC_y
Cuts at: 0 152 951
Size: 152 799
BtgI C'CryG_G
Cuts at: 0 35 269 576 951
Size: 35 234 307 375
Fragments arranged by size:
375 307 234 35
BtsI GCAGTG_nn'
Cuts at: 0 677 951
Size: 677 274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 329 364 951
Size: 329 35 587
Fragments arranged by size:
587 329 35
EaeI y'GGCC_r
Cuts at: 0 11 239 470 546 648 951
Size: 11 228 231 76 102 303
Fragments arranged by size:
303 231 228 102 76 11
EagI C'GGCC_G
Cuts at: 0 11 951
Size: 11 940
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 145 271 951
Size: 145 126 680
Fragments arranged by size:
680 145 126
EcoICRI GAG'CTC
Cuts at: 0 414 951
Size: 414 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 547 814 951
Size: 79 468 267 137
Fragments arranged by size:
468 267 137 79
EcoO109I rG'GnC_Cy
Cuts at: 0 42 43 951
Size: 42 1 908
Fragments arranged by size:
908 42 1
HaeII r_GCGC'y
Cuts at: 0 21 951
Size: 21 930
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 682 714 859 891 951
Size: 208 32 442 32 145 32 60
Fragments arranged by size:
442 208 145 60 32 32 32
HincII GTy'rAC
Cuts at: 0 729 951
Size: 729 222
MscI TGG'CCA
Cuts at: 0 241 472 548 951
Size: 241 231 76 403
Fragments arranged by size:
403 241 231 76
MslI CAynn'nnrTG
Cuts at: 0 274 951
Size: 274 677
MspA1I CmG'CkG
Cuts at: 0 95 804 813 951
Size: 95 709 9 138
Fragments arranged by size:
709 138 95 9
NcoI C'CATG_G
Cuts at: 0 35 269 576 951
Size: 35 234 307 375
Fragments arranged by size:
375 307 234 35
PmlI CAC'GTG
Cuts at: 0 554 951
Size: 554 397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 865 897 951
Size: 865 32 54
Fragments arranged by size:
865 54 32
PspOMI G'GGCC_C
Cuts at: 0 42 951
Size: 42 909
PspXI vC'TCGA_Gb
Cuts at: 0 160 951
Size: 160 791
PstI C_TGCA'G
Cuts at: 0 149 332 951
Size: 149 183 619
Fragments arranged by size:
619 183 149
PvuII CAG'CTG
Cuts at: 0 804 813 951
Size: 804 9 138
Fragments arranged by size:
804 138 9
SacI G_AGCT'C
Cuts at: 0 416 951
Size: 416 535
SexAI A'CCwGG_T
Cuts at: 0 692 951
Size: 692 259
SfcI C'TryA_G
Cuts at: 0 145 328 951
Size: 145 183 623
Fragments arranged by size:
623 183 145
SmlI C'TyrA_G
Cuts at: 0 160 951
Size: 160 791
StyI C'CwwG_G
Cuts at: 0 35 269 576 611 951
Size: 35 234 307 35 340
Fragments arranged by size:
340 307 234 35 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 951
Size: 604 347
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 951
Size: 502 32 417
Fragments arranged by size:
502 417 32
Tth111I GACn'n_nGTC
Cuts at: 0 492 951
Size: 492 459
XhoI C'TCGA_G
Cuts at: 0 160 951
Size: 160 791
XmnI GAAnn'nnTTC
Cuts at: 0 827 951
Size: 827 124
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApaI AvaI BanI
BanII BbvCI BciVI BclI Bme1580I Bpu10I BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BstAPI BstYI BtgI BtsI CspCI EaeI EagI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MscI
MslI MspA1I NcoI PmlI PpiI PspOMI PspXI PstI
PvuII SacI SexAI SfcI SmlI StyI TaqII TstI
Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpmI BpuEI BsaBI
BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EarI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpuMI PshAI PsiI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XcmI XmaI
ZraI