Restriction map for
pF1KB6450
(Linear) (Six-base) MAPSORT of: KIBB6450.seq Check: 9567 from: 1 to: 1047
KIBB6450.orf
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AfeI AGC'GCT
Cuts at: 0 695 1047
Size: 695 352
AleI CACnn'nnGTG
Cuts at: 0 380 1047
Size: 380 667
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 641 673 1047
Size: 641 32 374
Fragments arranged by size:
641 374 32
ApoI r'AATT_y
Cuts at: 0 469 497 1047
Size: 469 28 550
Fragments arranged by size:
550 469 28
AseI AT'TA_AT
Cuts at: 0 400 1047
Size: 400 647
AvaI C'yCGr_G
Cuts at: 0 668 734 740 1047
Size: 668 66 6 307
Fragments arranged by size:
668 307 66 6
BamHI G'GATC_C
Cuts at: 0 51 1047
Size: 51 996
BanI G'GyrC_C
Cuts at: 0 622 747 771 990 1047
Size: 622 125 24 219 57
Fragments arranged by size:
622 219 125 57 24
BanII G_rGCy'C
Cuts at: 0 741 1047
Size: 741 306
BbsI GAAGACnn'nnnn_
Cuts at: 0 134 1047
Size: 134 913
BciVI GTATCCnnnnn_n'
Cuts at: 0 221 757 1012 1047
Size: 221 536 255 35
Fragments arranged by size:
536 255 221 35
BfrBI ATG'CAT
Cuts at: 0 451 1047
Size: 451 596
BglI GCCn_nnn'nGGC
Cuts at: 0 745 1047
Size: 745 302
Bme1580I G_kGCm'C
Cuts at: 0 750 774 1047
Size: 750 24 273
Fragments arranged by size:
750 273 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 676 1047
Size: 676 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1047
Size: 767 280
Bpu10I CC'TnA_GC
Cuts at: 0 23 332 1047
Size: 23 309 715
Fragments arranged by size:
715 309 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1047
Size: 399 648
BsaAI yAC'GTr
Cuts at: 0 439 517 1047
Size: 439 78 530
Fragments arranged by size:
530 439 78
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 623 767 770 1047
Size: 22 601 144 3 277
Fragments arranged by size:
601 277 144 22 3
Bsp1286I G_dGCh'C
Cuts at: 0 741 750 774 1047
Size: 741 9 24 273
Fragments arranged by size:
741 273 24 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 962 1047
Size: 962 85
BstYI r'GATC_y
Cuts at: 0 51 1047
Size: 51 996
BtgI C'CryG_G
Cuts at: 0 615 687 1047
Size: 615 72 360
Fragments arranged by size:
615 360 72
DraIII CAC_nnn'GTG
Cuts at: 0 892 1047
Size: 892 155
EarI CTCTTCn'nnn_
Cuts at: 0 259 346 452 1047
Size: 259 87 106 595
Fragments arranged by size:
595 259 106 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1047
Size: 767 280
EcoNI CCTnn'n_nnAGG
Cuts at: 0 264 650 1047
Size: 264 386 397
Fragments arranged by size:
397 386 264
HaeII r_GCGC'y
Cuts at: 0 697 1047
Size: 697 350
HindIII A'AGCT_T
Cuts at: 0 428 794 1047
Size: 428 366 253
Fragments arranged by size:
428 366 253
MslI CAynn'nnrTG
Cuts at: 0 380 1034 1047
Size: 380 654 13
Fragments arranged by size:
654 380 13
NsiI A_TGCA'T
Cuts at: 0 453 1047
Size: 453 594
NspI r_CATG'y
Cuts at: 0 70 1047
Size: 70 977
PflMI CCAn_nnn'nTGG
Cuts at: 0 646 1047
Size: 646 401
PshAI GACnn'nnGTC
Cuts at: 0 551 914 1047
Size: 551 363 133
Fragments arranged by size:
551 363 133
PspXI vC'TCGA_Gb
Cuts at: 0 734 1047
Size: 734 313
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 202 234 1047
Size: 202 32 813
Fragments arranged by size:
813 202 32
ScaI AGT'ACT
Cuts at: 0 191 1047
Size: 191 856
SfcI C'TryA_G
Cuts at: 0 271 710 1047
Size: 271 439 337
Fragments arranged by size:
439 337 271
SmaI CCC'GGG
Cuts at: 0 742 1047
Size: 742 305
SmlI C'TyrA_G
Cuts at: 0 378 734 1047
Size: 378 356 313
Fragments arranged by size:
378 356 313
SphI G_CATG'C
Cuts at: 0 70 1047
Size: 70 977
SspI AAT'ATT
Cuts at: 0 16 1047
Size: 16 1031
TatI w'GTAC_w
Cuts at: 0 189 1047
Size: 189 858
XhoI C'TCGA_G
Cuts at: 0 734 1047
Size: 734 313
XmaI C'CCGG_G
Cuts at: 0 740 1047
Size: 740 307
XmnI GAAnn'nnTTC
Cuts at: 0 101 1047
Size: 101 946
Enzymes that do cut:
AfeI AleI AloI ApoI AseI AvaI BamHI BanI
BanII BbsI BciVI BfrBI BglI Bme1580I BplI BpmI
Bpu10I BpuEI BsaAI BseRI Bsp1286I BstXI BstYI BtgI
DraIII EarI Eco57MI EcoNI HaeII HindIII MslI NsiI
NspI PflMI PshAI PspXI PsrI ScaI SfcI SmaI
SmlI SphI SspI TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BbeI BbvCI BcgI BclI BglII BlpI
BmgBI BmrI BmtI BsaI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI ZraI