Restriction map for
pF1KB6455
(Linear) (Six-base) MAPSORT of: KIBB6455.seq Check: 5290 from: 1 to: 954
KIBB6455.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 346 954
Size: 346 608
AccI GT'mk_AC
Cuts at: 0 158 425 954
Size: 158 267 529
Fragments arranged by size:
529 267 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 224 954
Size: 3 221 730
Fragments arranged by size:
730 221 3
ApoI r'AATT_y
Cuts at: 0 100 398 954
Size: 100 298 556
Fragments arranged by size:
556 298 100
AseI AT'TA_AT
Cuts at: 0 221 954
Size: 221 733
BanI G'GyrC_C
Cuts at: 0 312 718 954
Size: 312 406 236
Fragments arranged by size:
406 312 236
BbeI G_GCGC'C
Cuts at: 0 316 722 954
Size: 316 406 232
Fragments arranged by size:
406 316 232
BclI T'GATC_A
Cuts at: 0 212 954
Size: 212 742
BmrI ACTGGGnnnn_n'
Cuts at: 0 415 954
Size: 415 539
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 954
Size: 492 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 954
Size: 94 860
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 954
Size: 109 845
BsaAI yAC'GTr
Cuts at: 0 145 954
Size: 145 809
BsaBI GATnn'nnATC
Cuts at: 0 871 954
Size: 871 83
BsaHI Gr'CG_yC
Cuts at: 0 313 719 954
Size: 313 406 235
Fragments arranged by size:
406 313 235
BsaWI w'CCGG_w
Cuts at: 0 760 954
Size: 760 194
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 140 170 908 938 954
Size: 140 30 738 30 16
Fragments arranged by size:
738 140 30 30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 477 954
Size: 380 97 477
Fragments arranged by size:
477 380 97
BspHI T'CATG_A
Cuts at: 0 215 954
Size: 215 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 346 954
Size: 346 608
BsrDI GCAATG_nn'
Cuts at: 0 539 846 954
Size: 539 307 108
Fragments arranged by size:
539 307 108
BsrFI r'CCGG_y
Cuts at: 0 444 954
Size: 444 510
BstAPI GCAn_nnn'nTGC
Cuts at: 0 686 954
Size: 686 268
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 654 954
Size: 603 51 300
Fragments arranged by size:
603 300 51
Bsu36I CC'TnA_GG
Cuts at: 0 827 954
Size: 827 127
BtgI C'CryG_G
Cuts at: 0 377 954
Size: 377 577
EaeI y'GGCC_r
Cuts at: 0 644 954
Size: 644 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 94 224 954
Size: 3 91 130 730
Fragments arranged by size:
730 130 91 3
EcoRV GAT'ATC
Cuts at: 0 417 954
Size: 417 537
HaeII r_GCGC'y
Cuts at: 0 316 722 954
Size: 316 406 232
Fragments arranged by size:
406 316 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 954
Size: 140 32 782
Fragments arranged by size:
782 140 32
HindIII A'AGCT_T
Cuts at: 0 328 954
Size: 328 626
KasI G'GCGC_C
Cuts at: 0 312 718 954
Size: 312 406 236
Fragments arranged by size:
406 312 236
MscI TGG'CCA
Cuts at: 0 646 954
Size: 646 308
MslI CAynn'nnrTG
Cuts at: 0 652 877 954
Size: 652 225 77
Fragments arranged by size:
652 225 77
MspA1I CmG'CkG
Cuts at: 0 23 697 715 954
Size: 23 674 18 239
Fragments arranged by size:
674 239 23 18
NaeI GCC'GGC
Cuts at: 0 446 954
Size: 446 508
NarI GG'CG_CC
Cuts at: 0 313 719 954
Size: 313 406 235
Fragments arranged by size:
406 313 235
NcoI C'CATG_G
Cuts at: 0 377 954
Size: 377 577
NgoMIV G'CCGG_C
Cuts at: 0 444 954
Size: 444 510
NspI r_CATG'y
Cuts at: 0 391 794 954
Size: 391 403 160
Fragments arranged by size:
403 391 160
PstI C_TGCA'G
Cuts at: 0 261 954
Size: 261 693
PvuII CAG'CTG
Cuts at: 0 697 715 954
Size: 697 18 239
Fragments arranged by size:
697 239 18
SfcI C'TryA_G
Cuts at: 0 257 350 954
Size: 257 93 604
Fragments arranged by size:
604 257 93
SfoI GGC'GCC
Cuts at: 0 314 720 954
Size: 314 406 234
Fragments arranged by size:
406 314 234
SphI G_CATG'C
Cuts at: 0 794 954
Size: 794 160
StyI C'CwwG_G
Cuts at: 0 377 954
Size: 377 577
Enzymes that do cut:
AarI AccI AcuI ApoI AseI BanI BbeI BclI
BmrI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsgI BspHI BspMI BsrDI BsrFI BstAPI BstXI
Bsu36I BtgI EaeI Eco57MI EcoRV HaeII Hin4I HindIII
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PstI PvuII SfcI SfoI SphI StyI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
Bpu10I BpuEI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI