Restriction map for
pF1KB6457
(Linear) (Six-base) MAPSORT of: KIBB6457.seq Check: 8438 from: 1 to: 954
KIBB6457.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 796 954
Size: 796 158
AclI AA'CG_TT
Cuts at: 0 39 954
Size: 39 915
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 954
Size: 40 914
ApaI G_GGCC'C
Cuts at: 0 143 771 954
Size: 143 628 183
Fragments arranged by size:
628 183 143
ApoI r'AATT_y
Cuts at: 0 690 954
Size: 690 264
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 184 217 954
Size: 184 33 737
Fragments arranged by size:
737 184 33
BanII G_rGCy'C
Cuts at: 0 143 754 771 954
Size: 143 611 17 183
Fragments arranged by size:
611 183 143 17
BbvCI CC'TCA_GC
Cuts at: 0 696 954
Size: 696 258
BciVI GTATCCnnnnn_n'
Cuts at: 0 212 954
Size: 212 742
BclI T'GATC_A
Cuts at: 0 846 954
Size: 846 108
Bme1580I G_kGCm'C
Cuts at: 0 143 771 954
Size: 143 628 183
Fragments arranged by size:
628 183 143
Bpu10I CC'TnA_GC
Cuts at: 0 696 954
Size: 696 258
BsaBI GATnn'nnATC
Cuts at: 0 874 954
Size: 874 80
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 179 954
Size: 179 775
BseYI C'CCAG_C
Cuts at: 0 770 954
Size: 770 184
BsiHKAI G_wGCw'C
Cuts at: 0 417 954
Size: 417 537
BsiWI C'GTAC_G
Cuts at: 0 677 954
Size: 677 277
Bsp1286I G_dGCh'C
Cuts at: 0 143 417 754 771 954
Size: 143 274 337 17 183
Fragments arranged by size:
337 274 183 143 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 954
Size: 534 420
BstYI r'GATC_y
Cuts at: 0 69 954
Size: 69 885
Bsu36I CC'TnA_GG
Cuts at: 0 401 954
Size: 401 553
DraI TTT'AAA
Cuts at: 0 488 954
Size: 488 466
EaeI y'GGCC_r
Cuts at: 0 665 954
Size: 665 289
EarI CTCTTCn'nnn_
Cuts at: 0 16 405 954
Size: 16 389 549
Fragments arranged by size:
549 389 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 195 954
Size: 195 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 954
Size: 40 914
EcoO109I rG'GnC_Cy
Cuts at: 0 139 140 954
Size: 139 1 814
Fragments arranged by size:
814 139 1
EcoRI G'AATT_C
Cuts at: 0 690 954
Size: 690 264
FspI TGC'GCA
Cuts at: 0 92 954
Size: 92 862
HaeII r_GCGC'y
Cuts at: 0 765 954
Size: 765 189
HindIII A'AGCT_T
Cuts at: 0 549 954
Size: 549 405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 933 954
Size: 97 836 21
Fragments arranged by size:
836 97 21
MslI CAynn'nnrTG
Cuts at: 0 86 954
Size: 86 868
MspA1I CmG'CkG
Cuts at: 0 862 954
Size: 862 92
PspOMI G'GGCC_C
Cuts at: 0 139 767 954
Size: 139 628 187
Fragments arranged by size:
628 187 139
PvuII CAG'CTG
Cuts at: 0 862 954
Size: 862 92
SapI GCTCTTCn'nnn_
Cuts at: 0 405 954
Size: 405 549
ScaI AGT'ACT
Cuts at: 0 188 954
Size: 188 766
SfcI C'TryA_G
Cuts at: 0 728 954
Size: 728 226
TatI w'GTAC_w
Cuts at: 0 186 670 864 954
Size: 186 484 194 90
Fragments arranged by size:
484 194 186 90
XbaI T'CTAG_A
Cuts at: 0 387 954
Size: 387 567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 847 954
Size: 847 107
XmnI GAAnn'nnTTC
Cuts at: 0 501 954
Size: 501 453
Enzymes that do cut:
AccI AclI AcuI ApaI ApoI BaeI BanII BbvCI
BciVI BclI Bme1580I Bpu10I BsaBI BseRI BseYI BsiHKAI
BsiWI Bsp1286I BstXI BstYI Bsu36I DraI EaeI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII HindIII MmeI
MslI MspA1I PspOMI PvuII SapI ScaI SfcI TatI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BanI BbeI BbsI BcgI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EcoICRI
EcoNI EcoRV FalI FseI FspAI Hin4I HincII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI Tth111I XhoI XmaI
ZraI