(Linear) (Six-base) MAPSORT of: KIBB6463.seq Check: 6439 from: 1 to: 966 KIBB6463.orf With 182 enzymes: SgfI * June 26, 2008 10:47 .. Acc65I G'GTAC_C Cuts at: 0 412 966 Size: 412 554 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 907 966 Size: 580 327 59 Fragments arranged by size: 580 327 59 AleI CACnn'nnGTG Cuts at: 0 848 966 Size: 848 118 ApoI r'AATT_y Cuts at: 0 178 393 431 760 966 Size: 178 215 38 329 206 Fragments arranged by size: 329 215 206 178 38 AseI AT'TA_AT Cuts at: 0 554 966 Size: 554 412 BamHI G'GATC_C Cuts at: 0 54 966 Size: 54 912 BanI G'GyrC_C Cuts at: 0 412 966 Size: 412 554 BbsI GAAGACnn'nnnn_ Cuts at: 0 116 344 966 Size: 116 228 622 Fragments arranged by size: 622 228 116 BglII A'GATC_T Cuts at: 0 279 966 Size: 279 687 Bme1580I G_kGCm'C Cuts at: 0 618 966 Size: 618 348 BmrI ACTGGGnnnn_n' Cuts at: 0 55 411 966 Size: 55 356 555 Fragments arranged by size: 555 356 55 BsaHI Gr'CG_yC Cuts at: 0 4 966 Size: 4 962 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 787 966 Size: 787 179 Bsp1286I G_dGCh'C Cuts at: 0 618 966 Size: 618 348 BspHI T'CATG_A Cuts at: 0 941 966 Size: 941 25 BsrDI GCAATG_nn' Cuts at: 0 351 966 Size: 351 615 BstXI CCAn_nnnn'nTGG Cuts at: 0 525 966 Size: 525 441 BstYI r'GATC_y Cuts at: 0 54 279 966 Size: 54 225 687 Fragments arranged by size: 687 225 54 Bsu36I CC'TnA_GG Cuts at: 0 788 966 Size: 788 178 BtgI C'CryG_G Cuts at: 0 26 966 Size: 26 940 BtsI GCAGTG_nn' Cuts at: 0 263 966 Size: 263 703 DraI TTT'AAA Cuts at: 0 392 506 966 Size: 392 114 460 Fragments arranged by size: 460 392 114 EarI CTCTTCn'nnn_ Cuts at: 0 74 137 810 966 Size: 74 63 673 156 Fragments arranged by size: 673 156 74 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 907 966 Size: 580 327 59 Fragments arranged by size: 580 327 59 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 666 966 Size: 666 300 HindIII A'AGCT_T Cuts at: 0 185 966 Size: 185 781 KpnI G_GTAC'C Cuts at: 0 416 966 Size: 416 550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 930 966 Size: 930 36 MslI CAynn'nnrTG Cuts at: 0 848 966 Size: 848 118 MspA1I CmG'CkG Cuts at: 0 492 966 Size: 492 474 PstI C_TGCA'G Cuts at: 0 236 270 966 Size: 236 34 696 Fragments arranged by size: 696 236 34 PvuII CAG'CTG Cuts at: 0 492 966 Size: 492 474 SfcI C'TryA_G Cuts at: 0 232 266 966 Size: 232 34 700 Fragments arranged by size: 700 232 34 SspI AAT'ATT Cuts at: 0 174 966 Size: 174 792 Tth111I GACn'n_nGTC Cuts at: 0 151 966 Size: 151 815 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 522 966 Size: 522 444 XmnI GAAnn'nnTTC Cuts at: 0 431 830 966 Size: 431 399 136 Fragments arranged by size: 431 399 136 Enzymes that do cut: Acc65I AcuI AleI ApoI AseI BamHI BanI BbsI BglII Bme1580I BmrI BsaHI BseRI Bsp1286I BspHI BsrDI BstXI BstYI Bsu36I BtgI BtsI DraI EarI Eco57MI FalI HindIII KpnI MmeI MslI MspA1I PstI PvuII SfcI SspI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HpaI KasI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TatI TstI XbaI XhoI XmaI ZraI