(Linear) (Six-base) MAPSORT of: KIBB6463.seq Check: 6439 from: 1 to: 966
KIBB6463.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
Acc65I G'GTAC_C
Cuts at: 0 412 966
Size: 412 554
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 907 966
Size: 580 327 59
Fragments arranged by size:
580 327 59
AleI CACnn'nnGTG
Cuts at: 0 848 966
Size: 848 118
ApoI r'AATT_y
Cuts at: 0 178 393 431 760 966
Size: 178 215 38 329 206
Fragments arranged by size:
329 215 206 178 38
AseI AT'TA_AT
Cuts at: 0 554 966
Size: 554 412
BamHI G'GATC_C
Cuts at: 0 54 966
Size: 54 912
BanI G'GyrC_C
Cuts at: 0 412 966
Size: 412 554
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 344 966
Size: 116 228 622
Fragments arranged by size:
622 228 116
BglII A'GATC_T
Cuts at: 0 279 966
Size: 279 687
Bme1580I G_kGCm'C
Cuts at: 0 618 966
Size: 618 348
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 411 966
Size: 55 356 555
Fragments arranged by size:
555 356 55
BsaHI Gr'CG_yC
Cuts at: 0 4 966
Size: 4 962
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 787 966
Size: 787 179
Bsp1286I G_dGCh'C
Cuts at: 0 618 966
Size: 618 348
BspHI T'CATG_A
Cuts at: 0 941 966
Size: 941 25
BsrDI GCAATG_nn'
Cuts at: 0 351 966
Size: 351 615
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 966
Size: 525 441
BstYI r'GATC_y
Cuts at: 0 54 279 966
Size: 54 225 687
Fragments arranged by size:
687 225 54
Bsu36I CC'TnA_GG
Cuts at: 0 788 966
Size: 788 178
BtgI C'CryG_G
Cuts at: 0 26 966
Size: 26 940
BtsI GCAGTG_nn'
Cuts at: 0 263 966
Size: 263 703
DraI TTT'AAA
Cuts at: 0 392 506 966
Size: 392 114 460
Fragments arranged by size:
460 392 114
EarI CTCTTCn'nnn_
Cuts at: 0 74 137 810 966
Size: 74 63 673 156
Fragments arranged by size:
673 156 74 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 907 966
Size: 580 327 59
Fragments arranged by size:
580 327 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 966
Size: 666 300
HindIII A'AGCT_T
Cuts at: 0 185 966
Size: 185 781
KpnI G_GTAC'C
Cuts at: 0 416 966
Size: 416 550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 930 966
Size: 930 36
MslI CAynn'nnrTG
Cuts at: 0 848 966
Size: 848 118
MspA1I CmG'CkG
Cuts at: 0 492 966
Size: 492 474
PstI C_TGCA'G
Cuts at: 0 236 270 966
Size: 236 34 696
Fragments arranged by size:
696 236 34
PvuII CAG'CTG
Cuts at: 0 492 966
Size: 492 474
SfcI C'TryA_G
Cuts at: 0 232 266 966
Size: 232 34 700
Fragments arranged by size:
700 232 34
SspI AAT'ATT
Cuts at: 0 174 966
Size: 174 792
Tth111I GACn'n_nGTC
Cuts at: 0 151 966
Size: 151 815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 522 966
Size: 522 444
XmnI GAAnn'nnTTC
Cuts at: 0 431 830 966
Size: 431 399 136
Fragments arranged by size:
431 399 136
Enzymes that do cut:
Acc65I AcuI AleI ApoI AseI BamHI BanI BbsI
BglII Bme1580I BmrI BsaHI BseRI Bsp1286I BspHI BsrDI
BstXI BstYI Bsu36I BtgI BtsI DraI EarI Eco57MI
FalI HindIII KpnI MmeI MslI MspA1I PstI PvuII
SfcI SspI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I HincII HpaI KasI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII TatI TstI XbaI XhoI XmaI ZraI