Restriction map for
pF1KB6466
(Linear) (Six-base) MAPSORT of: KIBB6466.seq Check: 6975 from: 1 to: 1056
KIBB6466.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
Acc65I G'GTAC_C
Cuts at: 0 372 1056
Size: 372 684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 416 620 1056
Size: 193 223 204 436
Fragments arranged by size:
436 223 204 193
AflIII A'CryG_T
Cuts at: 0 812 1056
Size: 812 244
AlwNI CAG_nnn'CTG
Cuts at: 0 831 1056
Size: 831 225
ApaI G_GGCC'C
Cuts at: 0 85 109 1056
Size: 85 24 947
Fragments arranged by size:
947 85 24
AvaI C'yCGr_G
Cuts at: 0 78 108 710 968 1056
Size: 78 30 602 258 88
Fragments arranged by size:
602 258 88 78 30
AvrII C'CTAG_G
Cuts at: 0 876 1056
Size: 876 180
BanI G'GyrC_C
Cuts at: 0 372 1056
Size: 372 684
BanII G_rGCy'C
Cuts at: 0 85 109 178 571 995 1056
Size: 85 24 69 393 424 61
Fragments arranged by size:
424 393 85 69 61 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 888 1056
Size: 888 168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 548 1056
Size: 514 34 508
Fragments arranged by size:
514 508 34
BclI T'GATC_A
Cuts at: 0 887 1056
Size: 887 169
BglII A'GATC_T
Cuts at: 0 158 1056
Size: 158 898
Bme1580I G_kGCm'C
Cuts at: 0 85 109 1056
Size: 85 24 947
Fragments arranged by size:
947 85 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1056
Size: 555 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1056
Size: 497 559
BsaHI Gr'CG_yC
Cuts at: 0 353 761 1056
Size: 353 408 295
Fragments arranged by size:
408 353 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 982 1012 1056
Size: 766 30 186 30 44
Fragments arranged by size:
766 186 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1003 1056
Size: 1003 53
BseYI C'CCAG_C
Cuts at: 0 301 982 1056
Size: 301 681 74
Fragments arranged by size:
681 301 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1056
Size: 910 146
BsiHKAI G_wGCw'C
Cuts at: 0 571 995 1056
Size: 571 424 61
Fragments arranged by size:
571 424 61
BsmI GAATG_Cn'
Cuts at: 0 200 1056
Size: 200 856
Bsp1286I G_dGCh'C
Cuts at: 0 85 109 178 571 995 1056
Size: 85 24 69 393 424 61
Fragments arranged by size:
424 393 85 69 61 24
BsrBI CCG'CTC
Cuts at: 0 99 1056
Size: 99 957
BstYI r'GATC_y
Cuts at: 0 158 1056
Size: 158 898
BtgI C'CryG_G
Cuts at: 0 735 800 1042 1056
Size: 735 65 242 14
Fragments arranged by size:
735 242 65 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 945 960 1056
Size: 438 507 15 96
Fragments arranged by size:
507 438 96 15
ClaI AT'CG_AT
Cuts at: 0 668 1056
Size: 668 388
EarI CTCTTCn'nnn_
Cuts at: 0 209 281 571 1056
Size: 209 72 290 485
Fragments arranged by size:
485 290 209 72
EcoICRI GAG'CTC
Cuts at: 0 569 993 1056
Size: 569 424 63
Fragments arranged by size:
569 424 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 416 555 620 1056
Size: 193 223 139 65 436
Fragments arranged by size:
436 223 193 139 65
EcoO109I rG'GnC_Cy
Cuts at: 0 105 116 364 495 741 792 1056
Size: 105 11 248 131 246 51 264
Fragments arranged by size:
264 248 246 131 105 51 11
HaeII r_GCGC'y
Cuts at: 0 323 1056
Size: 323 733
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1056
Size: 580 32 444
Fragments arranged by size:
580 444 32
KpnI G_GTAC'C
Cuts at: 0 376 1056
Size: 376 680
NspI r_CATG'y
Cuts at: 0 816 1056
Size: 816 240
PciI A'CATG_T
Cuts at: 0 812 1056
Size: 812 244
PfoI T'CCnGG_A
Cuts at: 0 571 1056
Size: 571 485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1056
Size: 172 32 852
Fragments arranged by size:
852 172 32
PpuMI rG'GwC_Cy
Cuts at: 0 364 741 792 1056
Size: 364 377 51 264
Fragments arranged by size:
377 364 264 51
PspOMI G'GGCC_C
Cuts at: 0 81 105 1056
Size: 81 24 951
Fragments arranged by size:
951 81 24
PstI C_TGCA'G
Cuts at: 0 596 1056
Size: 596 460
SacI G_AGCT'C
Cuts at: 0 571 995 1056
Size: 571 424 61
Fragments arranged by size:
571 424 61
SapI GCTCTTCn'nnn_
Cuts at: 0 571 1056
Size: 571 485
SfcI C'TryA_G
Cuts at: 0 273 592 1056
Size: 273 319 464
Fragments arranged by size:
464 319 273
SmaI CCC'GGG
Cuts at: 0 80 110 712 1056
Size: 80 30 602 344
Fragments arranged by size:
602 344 80 30
SmlI C'TyrA_G
Cuts at: 0 512 1056
Size: 512 544
StyI C'CwwG_G
Cuts at: 0 876 1056
Size: 876 180
TatI w'GTAC_w
Cuts at: 0 815 1056
Size: 815 241
XmaI C'CCGG_G
Cuts at: 0 78 108 710 1056
Size: 78 30 602 346
Fragments arranged by size:
602 346 78 30
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaI AvaI AvrII BanI
BanII BbsI BcgI BclI BglII Bme1580I BpmI BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrBI BstYI BtgI BtgZI ClaI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I KpnI NspI PciI PfoI PpiI
PpuMI PspOMI PstI SacI SapI SfcI SmaI SmlI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI