Restriction map for
pF1KB6469
(Linear) (Six-base) MAPSORT of: KIBB6469.seq Check: 8106 from: 1 to: 972
KIBB6469.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AatII G_ACGT'C
Cuts at: 0 698 972
Size: 698 274
Acc65I G'GTAC_C
Cuts at: 0 281 972
Size: 281 691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 972
Size: 772 200
AflIII A'CryG_T
Cuts at: 0 230 468 972
Size: 230 238 504
Fragments arranged by size:
504 238 230
AleI CACnn'nnGTG
Cuts at: 0 547 972
Size: 547 425
AvrII C'CTAG_G
Cuts at: 0 390 972
Size: 390 582
BanI G'GyrC_C
Cuts at: 0 281 972
Size: 281 691
BbsI GAAGACnn'nnnn_
Cuts at: 0 690 760 972
Size: 690 70 212
Fragments arranged by size:
690 212 70
BbvCI CC'TCA_GC
Cuts at: 0 500 972
Size: 500 472
Bme1580I G_kGCm'C
Cuts at: 0 556 972
Size: 556 416
Bpu10I CC'TnA_GC
Cuts at: 0 500 972
Size: 500 472
BsaAI yAC'GTr
Cuts at: 0 471 972
Size: 471 501
BsaHI Gr'CG_yC
Cuts at: 0 695 972
Size: 695 277
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 967 972
Size: 967 5
BseYI C'CCAG_C
Cuts at: 0 563 972
Size: 563 409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 799 972
Size: 37 762 173
Fragments arranged by size:
762 173 37
Bsp1286I G_dGCh'C
Cuts at: 0 556 972
Size: 556 416
BspHI T'CATG_A
Cuts at: 0 332 972
Size: 332 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 713 972
Size: 713 259
BsrDI GCAATG_nn'
Cuts at: 0 314 972
Size: 314 658
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 586 615 972
Size: 439 147 29 357
Fragments arranged by size:
439 357 147 29
BtgI C'CryG_G
Cuts at: 0 585 803 972
Size: 585 218 169
Fragments arranged by size:
585 218 169
BtsI GCAGTG_nn'
Cuts at: 0 20 972
Size: 20 952
EarI CTCTTCn'nnn_
Cuts at: 0 875 972
Size: 875 97
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 972
Size: 772 200
EcoO109I rG'GnC_Cy
Cuts at: 0 292 387 972
Size: 292 95 585
Fragments arranged by size:
585 292 95
EcoRV GAT'ATC
Cuts at: 0 534 621 972
Size: 534 87 351
Fragments arranged by size:
534 351 87
HindIII A'AGCT_T
Cuts at: 0 610 972
Size: 610 362
KpnI G_GTAC'C
Cuts at: 0 285 972
Size: 285 687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 472 489 972
Size: 66 406 17 483
Fragments arranged by size:
483 406 66 17
MslI CAynn'nnrTG
Cuts at: 0 308 373 418 437 547 972
Size: 308 65 45 19 110 425
Fragments arranged by size:
425 308 110 65 45 19
MspA1I CmG'CkG
Cuts at: 0 567 687 972
Size: 567 120 285
Fragments arranged by size:
567 285 120
NcoI C'CATG_G
Cuts at: 0 585 972
Size: 585 387
NspI r_CATG'y
Cuts at: 0 234 972
Size: 234 738
PciI A'CATG_T
Cuts at: 0 230 972
Size: 230 742
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 771 972
Size: 39 732 201
Fragments arranged by size:
732 201 39
PmlI CAC'GTG
Cuts at: 0 471 972
Size: 471 501
PpuMI rG'GwC_Cy
Cuts at: 0 387 972
Size: 387 585
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 509 541 972
Size: 509 32 431
Fragments arranged by size:
509 431 32
PstI C_TGCA'G
Cuts at: 0 692 728 972
Size: 692 36 244
Fragments arranged by size:
692 244 36
PvuII CAG'CTG
Cuts at: 0 687 972
Size: 687 285
SbfI CC_TGCA'GG
Cuts at: 0 728 972
Size: 728 244
ScaI AGT'ACT
Cuts at: 0 140 972
Size: 140 832
SexAI A'CCwGG_T
Cuts at: 0 578 972
Size: 578 394
SfcI C'TryA_G
Cuts at: 0 401 688 724 972
Size: 401 287 36 248
Fragments arranged by size:
401 287 248 36
StuI AGG'CCT
Cuts at: 0 742 972
Size: 742 230
StyI C'CwwG_G
Cuts at: 0 390 585 972
Size: 390 195 387
Fragments arranged by size:
390 387 195
TatI w'GTAC_w
Cuts at: 0 138 779 972
Size: 138 641 193
Fragments arranged by size:
641 193 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 972
Size: 765 207
ZraI GAC'GTC
Cuts at: 0 696 972
Size: 696 276
Enzymes that do cut:
AatII Acc65I AcuI AflIII AleI AvrII BanI BbsI
BbvCI Bme1580I Bpu10I BsaAI BsaHI BseRI BseYI BsgI
Bsp1286I BspHI BspMI BsrDI BstXI BtgI BtsI EarI
Eco57MI EcoO109I EcoRV HindIII KpnI MmeI MslI MspA1I
NcoI NspI PciI PflMI PmlI PpuMI PsrI PstI
PvuII SbfI ScaI SexAI SfcI StuI StyI TatI
XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BanII BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII HpaI KasI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI XmnI