Restriction map for
pF1KB6470
(Linear) (Six-base) MAPSORT of: KIBB6470.seq Check: 4400 from: 1 to: 1062
KIBB6470.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
Acc65I G'GTAC_C
Cuts at: 0 630 1062
Size: 630 432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 1062
Size: 54 1008
AflIII A'CryG_T
Cuts at: 0 749 1062
Size: 749 313
AlwNI CAG_nnn'CTG
Cuts at: 0 696 1062
Size: 696 366
ApaI G_GGCC'C
Cuts at: 0 229 276 286 1062
Size: 229 47 10 776
Fragments arranged by size:
776 229 47 10
AvaI C'yCGr_G
Cuts at: 0 269 1062
Size: 269 793
BanI G'GyrC_C
Cuts at: 0 630 1062
Size: 630 432
BanII G_rGCy'C
Cuts at: 0 229 276 286 450 539 773 1062
Size: 229 47 10 164 89 234 289
Fragments arranged by size:
289 234 229 164 89 47 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 1045 1062
Size: 167 878 17
Fragments arranged by size:
878 167 17
BciVI GTATCCnnnnn_n'
Cuts at: 0 955 1062
Size: 955 107
BglI GCCn_nnn'nGGC
Cuts at: 0 1054 1062
Size: 1054 8
Bme1580I G_kGCm'C
Cuts at: 0 209 229 276 286 1062
Size: 209 20 47 10 776
Fragments arranged by size:
776 209 47 20 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 961 1062
Size: 215 746 101
Fragments arranged by size:
746 215 101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1062
Size: 480 582
BsaHI Gr'CG_yC
Cuts at: 0 584 1062
Size: 584 478
BsaWI w'CCGG_w
Cuts at: 0 563 1062
Size: 563 499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 477 1062
Size: 477 585
BseYI C'CCAG_C
Cuts at: 0 279 285 449 1062
Size: 279 6 164 613
Fragments arranged by size:
613 279 164 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1062
Size: 575 487
BsmI GAATG_Cn'
Cuts at: 0 254 819 1062
Size: 254 565 243
Fragments arranged by size:
565 254 243
Bsp1286I G_dGCh'C
Cuts at: 0 209 229 276 286 450 539 773 1062
Size: 209 20 47 10 164 89 234 289
Fragments arranged by size:
289 234 209 164 89 47 20 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 713 1062
Size: 713 349
BsrDI GCAATG_nn'
Cuts at: 0 218 1062
Size: 218 844
BsrFI r'CCGG_y
Cuts at: 0 91 238 1062
Size: 91 147 824
Fragments arranged by size:
824 147 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 260 1062
Size: 260 802
BstYI r'GATC_y
Cuts at: 0 590 1062
Size: 590 472
BtgI C'CryG_G
Cuts at: 0 109 470 1062
Size: 109 361 592
Fragments arranged by size:
592 361 109
BtsI GCAGTG_nn'
Cuts at: 0 778 1062
Size: 778 284
DraIII CAC_nnn'GTG
Cuts at: 0 810 1062
Size: 810 252
EaeI y'GGCC_r
Cuts at: 0 93 1055 1062
Size: 93 962 7
Fragments arranged by size:
962 93 7
EarI CTCTTCn'nnn_
Cuts at: 0 392 1062
Size: 392 670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 215 961 1062
Size: 54 161 746 101
Fragments arranged by size:
746 161 101 54
EcoO109I rG'GnC_Cy
Cuts at: 0 78 226 273 1062
Size: 78 148 47 789
Fragments arranged by size:
789 148 78 47
EcoRV GAT'ATC
Cuts at: 0 882 1062
Size: 882 180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 403 1062
Size: 403 659
HaeII r_GCGC'y
Cuts at: 0 92 697 1062
Size: 92 605 365
Fragments arranged by size:
605 365 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 831 863 1062
Size: 392 32 407 32 199
Fragments arranged by size:
407 392 199 32 32
KpnI G_GTAC'C
Cuts at: 0 634 1062
Size: 634 428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1062
Size: 287 775
MscI TGG'CCA
Cuts at: 0 1057 1062
Size: 1057 5
MslI CAynn'nnrTG
Cuts at: 0 970 1062
Size: 970 92
MspA1I CmG'CkG
Cuts at: 0 551 1062
Size: 551 511
NaeI GCC'GGC
Cuts at: 0 93 1062
Size: 93 969
NcoI C'CATG_G
Cuts at: 0 470 1062
Size: 470 592
NgoMIV G'CCGG_C
Cuts at: 0 91 1062
Size: 91 971
PasI CC'CwG_GG
Cuts at: 0 675 1062
Size: 675 387
PfoI T'CCnGG_A
Cuts at: 0 231 1062
Size: 231 831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 1062
Size: 736 32 294
Fragments arranged by size:
736 294 32
PspOMI G'GGCC_C
Cuts at: 0 225 272 282 1062
Size: 225 47 10 780
Fragments arranged by size:
780 225 47 10
PstI C_TGCA'G
Cuts at: 0 710 1062
Size: 710 352
SfcI C'TryA_G
Cuts at: 0 706 1062
Size: 706 356
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1054 1062
Size: 1054 8
SmaI CCC'GGG
Cuts at: 0 271 1062
Size: 271 791
SmlI C'TyrA_G
Cuts at: 0 459 1062
Size: 459 603
SrfI GCCC'GGGC
Cuts at: 0 271 1062
Size: 271 791
StuI AGG'CCT
Cuts at: 0 1016 1062
Size: 1016 46
StyI C'CwwG_G
Cuts at: 0 470 1062
Size: 470 592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 595 791 1062
Size: 454 141 196 271
Fragments arranged by size:
454 271 196 141
XmaI C'CCGG_G
Cuts at: 0 269 1062
Size: 269 793
XmnI GAAnn'nnTTC
Cuts at: 0 751 1062
Size: 751 311
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbsI BciVI BglI Bme1580I BpmI BpuEI BsaHI BsaWI
BseRI BseYI BsgI BsmI Bsp1286I BspMI BsrDI BsrFI
BstAPI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV PasI PfoI PpiI
PspOMI PstI SfcI SfiI SmaI SmlI SrfI StuI
StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI