Restriction map for
pF1KB6472
(Linear) (Six-base) MAPSORT of: KIBB6472.seq Check: 1391 from: 1 to: 1065
KIBB6472.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 218 1065
Size: 218 847
AfeI AGC'GCT
Cuts at: 0 43 1065
Size: 43 1022
AflIII A'CryG_T
Cuts at: 0 785 1065
Size: 785 280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 338 348 1065
Size: 338 10 717
Fragments arranged by size:
717 338 10
BamHI G'GATC_C
Cuts at: 0 635 1065
Size: 635 430
BbvCI CC'TCA_GC
Cuts at: 0 707 1065
Size: 707 358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 439 473 1065
Size: 439 34 592
Fragments arranged by size:
592 439 34
BfrBI ATG'CAT
Cuts at: 0 732 1065
Size: 732 333
BglII A'GATC_T
Cuts at: 0 956 1065
Size: 956 109
BmgBI CAC'GTC
Cuts at: 0 788 1065
Size: 788 277
BmtI G_CTAG'C
Cuts at: 0 706 1065
Size: 706 359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 499 1065
Size: 279 220 566
Fragments arranged by size:
566 279 220
Bpu10I CC'TnA_GC
Cuts at: 0 707 1065
Size: 707 358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 702 1065
Size: 414 288 363
Fragments arranged by size:
414 363 288
BsaAI yAC'GTr
Cuts at: 0 974 1065
Size: 974 91
BsaBI GATnn'nnATC
Cuts at: 0 160 1065
Size: 160 905
BsaHI Gr'CG_yC
Cuts at: 0 308 377 653 1013 1065
Size: 308 69 276 360 52
Fragments arranged by size:
360 308 276 69 52
BsaWI w'CCGG_w
Cuts at: 0 383 1065
Size: 383 682
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 1065
Size: 88 30 947
Fragments arranged by size:
947 88 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 358 730 841 1065
Size: 202 156 372 111 224
Fragments arranged by size:
372 224 202 156 111
BseYI C'CCAG_C
Cuts at: 0 121 1065
Size: 121 944
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 430 1018 1065
Size: 318 112 588 47
Fragments arranged by size:
588 318 112 47
BsiEI CG_ry'CG
Cuts at: 0 38 1065
Size: 38 1027
BsiHKAI G_wGCw'C
Cuts at: 0 142 359 1065
Size: 142 217 706
Fragments arranged by size:
706 217 142
BsmI GAATG_Cn'
Cuts at: 0 199 1065
Size: 199 866
Bsp1286I G_dGCh'C
Cuts at: 0 142 359 1065
Size: 142 217 706
Fragments arranged by size:
706 217 142
BspEI T'CCGG_A
Cuts at: 0 383 1065
Size: 383 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 278 1065
Size: 74 204 787
Fragments arranged by size:
787 204 74
BsrBI CCG'CTC
Cuts at: 0 301 428 624 1065
Size: 301 127 196 441
Fragments arranged by size:
441 301 196 127
BsrFI r'CCGG_y
Cuts at: 0 197 1065
Size: 197 868
BssSI C'ACGA_G
Cuts at: 0 169 1065
Size: 169 896
BstAPI GCAn_nnn'nTGC
Cuts at: 0 354 522 1065
Size: 354 168 543
Fragments arranged by size:
543 354 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 1065
Size: 175 890
BstYI r'GATC_y
Cuts at: 0 635 956 1065
Size: 635 321 109
Fragments arranged by size:
635 321 109
BtgI C'CryG_G
Cuts at: 0 260 402 545 1065
Size: 260 142 143 520
Fragments arranged by size:
520 260 143 142
BtsI GCAGTG_nn'
Cuts at: 0 639 1065
Size: 639 426
DraIII CAC_nnn'GTG
Cuts at: 0 496 1065
Size: 496 569
EaeI y'GGCC_r
Cuts at: 0 35 245 423 1065
Size: 35 210 178 642
Fragments arranged by size:
642 210 178 35
EagI C'GGCC_G
Cuts at: 0 35 1065
Size: 35 1030
EarI CTCTTCn'nnn_
Cuts at: 0 130 805 1065
Size: 130 675 260
Fragments arranged by size:
675 260 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 499 1065
Size: 279 220 566
Fragments arranged by size:
566 279 220
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 823 1065
Size: 70 753 242
Fragments arranged by size:
753 242 70
EcoO109I rG'GnC_Cy
Cuts at: 0 579 816 927 1065
Size: 579 237 111 138
Fragments arranged by size:
579 237 138 111
HaeII r_GCGC'y
Cuts at: 0 20 45 689 1065
Size: 20 25 644 376
Fragments arranged by size:
644 376 25 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 355 387 1065
Size: 166 32 157 32 678
Fragments arranged by size:
678 166 157 32 32
MscI TGG'CCA
Cuts at: 0 247 1065
Size: 247 818
MslI CAynn'nnrTG
Cuts at: 0 173 1065
Size: 173 892
MspA1I CmG'CkG
Cuts at: 0 457 615 1065
Size: 457 158 450
Fragments arranged by size:
457 450 158
NaeI GCC'GGC
Cuts at: 0 199 1065
Size: 199 866
NcoI C'CATG_G
Cuts at: 0 260 402 1065
Size: 260 142 663
Fragments arranged by size:
663 260 142
NgoMIV G'CCGG_C
Cuts at: 0 197 1065
Size: 197 868
NheI G'CTAG_C
Cuts at: 0 702 1065
Size: 702 363
NsiI A_TGCA'T
Cuts at: 0 734 1065
Size: 734 331
NspI r_CATG'y
Cuts at: 0 732 1065
Size: 732 333
PmlI CAC'GTG
Cuts at: 0 974 1065
Size: 974 91
PpuMI rG'GwC_Cy
Cuts at: 0 579 816 927 1065
Size: 579 237 111 138
Fragments arranged by size:
579 237 138 111
PstI C_TGCA'G
Cuts at: 0 275 1065
Size: 275 790
PvuII CAG'CTG
Cuts at: 0 457 1065
Size: 457 608
SapI GCTCTTCn'nnn_
Cuts at: 0 130 1065
Size: 130 935
SfcI C'TryA_G
Cuts at: 0 219 271 1065
Size: 219 52 794
Fragments arranged by size:
794 219 52
SmlI C'TyrA_G
Cuts at: 0 429 681 1065
Size: 429 252 384
Fragments arranged by size:
429 384 252
SphI G_CATG'C
Cuts at: 0 732 1065
Size: 732 333
StuI AGG'CCT
Cuts at: 0 415 1065
Size: 415 650
StyI C'CwwG_G
Cuts at: 0 260 402 950 1065
Size: 260 142 548 115
Fragments arranged by size:
548 260 142 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 595 1065
Size: 106 489 470
Fragments arranged by size:
489 470 106
TatI w'GTAC_w
Cuts at: 0 869 1065
Size: 869 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 828 860 1065
Size: 828 32 205
Fragments arranged by size:
828 205 32
Tth111I GACn'n_nGTC
Cuts at: 0 1015 1065
Size: 1015 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 1065
Size: 552 513
XmnI GAAnn'nnTTC
Cuts at: 0 748 1065
Size: 748 317
Enzymes that do cut:
AccI AfeI AflIII AlfI BamHI BbvCI BcgI BfrBI
BglII BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI
Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NsiI NspI PmlI PpuMI
PstI PvuII SapI SfcI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI
BciVI BclI BglI BlpI Bme1580I BmrI BplI BsaI
BsiWI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NdeI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI XbaI XhoI XmaI ZraI