Restriction map for
pF1KB6474
(Linear) (Six-base) MAPSORT of: KIBB6474.seq Check: 4660 from: 1 to: 1065
KIBB6474.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 617 1065
Size: 617 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 176 1065
Size: 33 143 889
Fragments arranged by size:
889 143 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 997 1065
Size: 997 68
ApoI r'AATT_y
Cuts at: 0 316 433 1065
Size: 316 117 632
Fragments arranged by size:
632 316 117
BamHI G'GATC_C
Cuts at: 0 819 1065
Size: 819 246
BanII G_rGCy'C
Cuts at: 0 1064 1065
Size: 1064 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 164 1065
Size: 56 108 901
Fragments arranged by size:
901 108 56
BciVI GTATCCnnnnn_n'
Cuts at: 0 221 1065
Size: 221 844
BclI T'GATC_A
Cuts at: 0 546 1065
Size: 546 519
Bpu10I CC'TnA_GC
Cuts at: 0 524 1065
Size: 524 541
BsaI GGTCTCn'nnnn_
Cuts at: 0 827 1065
Size: 827 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 753 783 1065
Size: 753 30 282
Fragments arranged by size:
753 282 30
BsiHKAI G_wGCw'C
Cuts at: 0 530 1064 1065
Size: 530 534 1
Fragments arranged by size:
534 530 1
Bsp1286I G_dGCh'C
Cuts at: 0 530 1064 1065
Size: 530 534 1
Fragments arranged by size:
534 530 1
BsrGI T'GTAC_A
Cuts at: 0 1007 1065
Size: 1007 58
BssSI C'ACGA_G
Cuts at: 0 799 1065
Size: 799 266
BstBI TT'CG_AA
Cuts at: 0 915 1065
Size: 915 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 859 1065
Size: 859 206
BstYI r'GATC_y
Cuts at: 0 279 819 1065
Size: 279 540 246
Fragments arranged by size:
540 279 246
DraI TTT'AAA
Cuts at: 0 432 1065
Size: 432 633
EarI CTCTTCn'nnn_
Cuts at: 0 52 1065
Size: 52 1013
EcoICRI GAG'CTC
Cuts at: 0 1062 1065
Size: 1062 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 176 1065
Size: 33 143 889
Fragments arranged by size:
889 143 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 827 1065
Size: 827 238
EcoO109I rG'GnC_Cy
Cuts at: 0 520 1065
Size: 520 545
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 397 429 753 785 796 828
Size: 29 32 336 32 324 32 11 32
Cuts at: 828 1065
Size: 237
Fragments arranged by size:
336 324 237 32 32 32 32 29
11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 307 825 1065
Size: 272 35 518 240
Fragments arranged by size:
518 272 240 35
MslI CAynn'nnrTG
Cuts at: 0 404 533 740 814 857 1065
Size: 404 129 207 74 43 208
Fragments arranged by size:
404 208 207 129 74 43
NspI r_CATG'y
Cuts at: 0 754 1065
Size: 754 311
PfoI T'CCnGG_A
Cuts at: 0 213 243 1065
Size: 213 30 822
Fragments arranged by size:
822 213 30
PpuMI rG'GwC_Cy
Cuts at: 0 520 1065
Size: 520 545
SacI G_AGCT'C
Cuts at: 0 1064 1065
Size: 1064 1
SfcI C'TryA_G
Cuts at: 0 621 1065
Size: 621 444
SphI G_CATG'C
Cuts at: 0 754 1065
Size: 754 311
TatI w'GTAC_w
Cuts at: 0 991 1007 1065
Size: 991 16 58
Fragments arranged by size:
991 58 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 417 1065
Size: 384 33 648
Fragments arranged by size:
648 384 33
XmnI GAAnn'nnTTC
Cuts at: 0 871 1065
Size: 871 194
Enzymes that do cut:
AccI AcuI AlfI ApoI BamHI BanII BbsI BciVI
BclI Bpu10I BsaI BsaXI BsiHKAI Bsp1286I BsrGI BssSI
BstBI BstXI BstYI DraI EarI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I MmeI MslI NspI PfoI PpuMI SacI
SfcI SphI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI ZraI