Restriction map for
pF1KB6476
(Linear) (Six-base) MAPSORT of: KIBB6476.seq Check: 1450 from: 1 to: 1065
KIBB6476.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 252 1065
Size: 252 813
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 181 436 1065
Size: 116 65 255 629
Fragments arranged by size:
629 255 116 65
AlwNI CAG_nnn'CTG
Cuts at: 0 729 1065
Size: 729 336
ApoI r'AATT_y
Cuts at: 0 118 170 589 1065
Size: 118 52 419 476
Fragments arranged by size:
476 419 118 52
AseI AT'TA_AT
Cuts at: 0 422 1031 1065
Size: 422 609 34
Fragments arranged by size:
609 422 34
AvaI C'yCGr_G
Cuts at: 0 334 1065
Size: 334 731
BanI G'GyrC_C
Cuts at: 0 44 1065
Size: 44 1021
BanII G_rGCy'C
Cuts at: 0 335 824 1065
Size: 335 489 241
Fragments arranged by size:
489 335 241
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 347 859 1065
Size: 274 73 512 206
Fragments arranged by size:
512 274 206 73
BbvCI CC'TCA_GC
Cuts at: 0 398 1065
Size: 398 667
BciVI GTATCCnnnnn_n'
Cuts at: 0 271 1065
Size: 271 794
BfrBI ATG'CAT
Cuts at: 0 361 1065
Size: 361 704
BlpI GC'TnA_GC
Cuts at: 0 221 1065
Size: 221 844
Bpu10I CC'TnA_GC
Cuts at: 0 398 1065
Size: 398 667
BsaI GGTCTCn'nnnn_
Cuts at: 0 904 1065
Size: 904 161
BsaAI yAC'GTr
Cuts at: 0 1038 1065
Size: 1038 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 801 831 1065
Size: 801 30 234
Fragments arranged by size:
801 234 30
BseYI C'CCAG_C
Cuts at: 0 16 300 1065
Size: 16 284 765
Fragments arranged by size:
765 284 16
BsiHKAI G_wGCw'C
Cuts at: 0 335 824 1065
Size: 335 489 241
Fragments arranged by size:
489 335 241
BsmI GAATG_Cn'
Cuts at: 0 236 1065
Size: 236 829
Bsp1286I G_dGCh'C
Cuts at: 0 335 824 1065
Size: 335 489 241
Fragments arranged by size:
489 335 241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 252 1065
Size: 252 813
BsrDI GCAATG_nn'
Cuts at: 0 363 1065
Size: 363 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 729 1065
Size: 729 336
BstYI r'GATC_y
Cuts at: 0 251 1065
Size: 251 814
BtgI C'CryG_G
Cuts at: 0 875 1065
Size: 875 190
DraI TTT'AAA
Cuts at: 0 476 942 1065
Size: 476 466 123
Fragments arranged by size:
476 466 123
DrdI GACnn_nn'nnGTC
Cuts at: 0 568 1065
Size: 568 497
EarI CTCTTCn'nnn_
Cuts at: 0 590 1065
Size: 590 475
EcoICRI GAG'CTC
Cuts at: 0 333 822 1065
Size: 333 489 243
Fragments arranged by size:
489 333 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 181 436 1065
Size: 116 65 255 629
Fragments arranged by size:
629 255 116 65
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 430 1065
Size: 430 635
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 474 506 1065
Size: 474 32 559
Fragments arranged by size:
559 474 32
MslI CAynn'nnrTG
Cuts at: 0 874 1065
Size: 874 191
MspA1I CmG'CkG
Cuts at: 0 760 1065
Size: 760 305
NcoI C'CATG_G
Cuts at: 0 875 1065
Size: 875 190
NsiI A_TGCA'T
Cuts at: 0 363 1065
Size: 363 702
NspI r_CATG'y
Cuts at: 0 627 1065
Size: 627 438
PsiI TTA'TAA
Cuts at: 0 533 1065
Size: 533 532
PstI C_TGCA'G
Cuts at: 0 725 734 1065
Size: 725 9 331
Fragments arranged by size:
725 331 9
PvuII CAG'CTG
Cuts at: 0 760 1065
Size: 760 305
SacI G_AGCT'C
Cuts at: 0 335 824 1065
Size: 335 489 241
Fragments arranged by size:
489 335 241
SfcI C'TryA_G
Cuts at: 0 721 730 742 845 1065
Size: 721 9 12 103 220
Fragments arranged by size:
721 220 103 12 9
StuI AGG'CCT
Cuts at: 0 798 1065
Size: 798 267
StyI C'CwwG_G
Cuts at: 0 793 875 924 1065
Size: 793 82 49 141
Fragments arranged by size:
793 141 82 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 801 833 1065
Size: 691 32 78 32 232
Fragments arranged by size:
691 232 78 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 390 1035 1065
Size: 390 645 30
Fragments arranged by size:
645 390 30
XmnI GAAnn'nnTTC
Cuts at: 0 194 1065
Size: 194 871
Enzymes that do cut:
AarI AcuI AlwNI ApoI AseI AvaI BanI BanII
BbsI BbvCI BciVI BfrBI BlpI Bpu10I BsaI BsaAI
BsaXI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstAPI
BstYI BtgI DraI DrdI EarI EcoICRI Eco57MI FalI
Hin4I MslI MspA1I NcoI NsiI NspI PsiI PstI
PvuII SacI SfcI StuI StyI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BcgI BclI BglI BglII
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI ZraI