Restriction map for
pF1KB6478
(Linear) (Six-base) MAPSORT of: KIBB6478.seq Check: 6688 from: 1 to: 1065
KIBB6478.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 496 1065
Size: 174 322 569
Fragments arranged by size:
569 322 174
AflIII A'CryG_T
Cuts at: 0 404 1065
Size: 404 661
AleI CACnn'nnGTG
Cuts at: 0 152 457 1065
Size: 152 305 608
Fragments arranged by size:
608 305 152
BanI G'GyrC_C
Cuts at: 0 154 463 1065
Size: 154 309 602
Fragments arranged by size:
602 309 154
BanII G_rGCy'C
Cuts at: 0 353 395 896 956 983 1065
Size: 353 42 501 60 27 82
Fragments arranged by size:
501 353 82 60 42 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 122 1065
Size: 122 943
BbvCI CC'TCA_GC
Cuts at: 0 541 1065
Size: 541 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 727 761 1065
Size: 727 34 304
Fragments arranged by size:
727 304 34
BglI GCCn_nnn'nGGC
Cuts at: 0 604 698 1065
Size: 604 94 367
Fragments arranged by size:
604 367 94
BglII A'GATC_T
Cuts at: 0 65 1065
Size: 65 1000
Bme1580I G_kGCm'C
Cuts at: 0 466 1065
Size: 466 599
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 1065
Size: 570 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 322 832 865 910 1065
Size: 109 213 510 33 45 155
Fragments arranged by size:
510 213 155 109 45 33
Bpu10I CC'TnA_GC
Cuts at: 0 363 506 541 1065
Size: 363 143 35 524
Fragments arranged by size:
524 363 143 35
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 235 307 1065
Size: 97 138 72 758
Fragments arranged by size:
758 138 97 72
BseYI C'CCAG_C
Cuts at: 0 636 661 962 1060 1065
Size: 636 25 301 98 5
Fragments arranged by size:
636 301 98 25 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 480 1065
Size: 316 164 585
Fragments arranged by size:
585 316 164
BsiEI CG_ry'CG
Cuts at: 0 582 741 1065
Size: 582 159 324
Fragments arranged by size:
582 324 159
BsiHKAI G_wGCw'C
Cuts at: 0 395 1065
Size: 395 670
BsiWI C'GTAC_G
Cuts at: 0 32 1065
Size: 32 1033
Bsp1286I G_dGCh'C
Cuts at: 0 353 395 466 896 956 983 1065
Size: 353 42 71 430 60 27 82
Fragments arranged by size:
430 353 82 71 60 42 27
BspHI T'CATG_A
Cuts at: 0 782 794 1065
Size: 782 12 271
Fragments arranged by size:
782 271 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 728 1065
Size: 680 48 337
Fragments arranged by size:
680 337 48
BsrBI CCG'CTC
Cuts at: 0 93 1002 1065
Size: 93 909 63
Fragments arranged by size:
909 93 63
BsrFI r'CCGG_y
Cuts at: 0 157 488 937 1065
Size: 157 331 449 128
Fragments arranged by size:
449 331 157 128
BssHII G'CGCG_C
Cuts at: 0 263 1065
Size: 263 802
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 1065
Size: 636 429
BstYI r'GATC_y
Cuts at: 0 65 653 815 1065
Size: 65 588 162 250
Fragments arranged by size:
588 250 162 65
BtgI C'CryG_G
Cuts at: 0 201 215 396 467 482 500 762 1065
Size: 201 14 181 71 15 18 262 303
Fragments arranged by size:
303 262 201 181 71 18 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 629 1065
Size: 629 436
BtsI GCAGTG_nn'
Cuts at: 0 167 1065
Size: 167 898
EcoICRI GAG'CTC
Cuts at: 0 393 1065
Size: 393 672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 174 322 496 832 865 910 1065
Size: 109 65 148 174 336 33 45 155
Fragments arranged by size:
336 174 155 148 109 65 45 33
EcoO109I rG'GnC_Cy
Cuts at: 0 196 585 1065
Size: 196 389 480
Fragments arranged by size:
480 389 196
HaeII r_GCGC'y
Cuts at: 0 292 693 856 1065
Size: 292 401 163 209
Fragments arranged by size:
401 292 209 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 440 472 1065
Size: 440 32 593
Fragments arranged by size:
593 440 32
HincII GTy'rAC
Cuts at: 0 717 774 1065
Size: 717 57 291
Fragments arranged by size:
717 291 57
MslI CAynn'nnrTG
Cuts at: 0 152 409 457 1065
Size: 152 257 48 608
Fragments arranged by size:
608 257 152 48
MspA1I CmG'CkG
Cuts at: 0 300 665 678 1065
Size: 300 365 13 387
Fragments arranged by size:
387 365 300 13
NaeI GCC'GGC
Cuts at: 0 159 490 939 1065
Size: 159 331 449 126
Fragments arranged by size:
449 331 159 126
NcoI C'CATG_G
Cuts at: 0 215 467 762 1065
Size: 215 252 295 303
Fragments arranged by size:
303 295 252 215
NgoMIV G'CCGG_C
Cuts at: 0 157 488 937 1065
Size: 157 331 449 128
Fragments arranged by size:
449 331 157 128
NspI r_CATG'y
Cuts at: 0 408 1065
Size: 408 657
PasI CC'CwG_GG
Cuts at: 0 374 1065
Size: 374 691
PciI A'CATG_T
Cuts at: 0 404 1065
Size: 404 661
PflMI CCAn_nnn'nTGG
Cuts at: 0 342 1065
Size: 342 723
PfoI T'CCnGG_A
Cuts at: 0 59 1065
Size: 59 1006
PpuMI rG'GwC_Cy
Cuts at: 0 196 585 1065
Size: 196 389 480
Fragments arranged by size:
480 389 196
PstI C_TGCA'G
Cuts at: 0 532 653 725 1065
Size: 532 121 72 340
Fragments arranged by size:
532 340 121 72
PvuII CAG'CTG
Cuts at: 0 300 1065
Size: 300 765
SacI G_AGCT'C
Cuts at: 0 395 1065
Size: 395 670
SanDI GG'GwC_CC
Cuts at: 0 196 1065
Size: 196 869
SbfI CC_TGCA'GG
Cuts at: 0 532 1065
Size: 532 533
SfcI C'TryA_G
Cuts at: 0 528 649 721 1065
Size: 528 121 72 344
Fragments arranged by size:
528 344 121 72
StyI C'CwwG_G
Cuts at: 0 215 467 762 896 1049 1065
Size: 215 252 295 134 153 16
Fragments arranged by size:
295 252 215 153 134 16
Tth111I GACn'n_nGTC
Cuts at: 0 454 778 1065
Size: 454 324 287
Fragments arranged by size:
454 324 287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 885 1065
Size: 381 504 180
Fragments arranged by size:
504 381 180
Enzymes that do cut:
AcuI AflIII AleI BanI BanII BbsI BbvCI BcgI
BglI BglII Bme1580I BmrI BpmI Bpu10I BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BstXI BstYI BtgI BtgZI BtsI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PstI PvuII SacI SanDI SbfI SfcI StyI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsmI BsmBI BspEI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI XbaI XhoI
XmaI XmnI ZraI