Restriction map for
pF1KB6480
(Linear) (Six-base) MAPSORT of: KIBB6480.seq Check: 121 from: 1 to: 1011
KIBB6480.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 0 947 1011
Size: 947 64
AccI GT'mk_AC
Cuts at: 0 185 1011
Size: 185 826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1011
Size: 718 293
AhdI GACnn_n'nnGTC
Cuts at: 0 237 957 1011
Size: 237 720 54
Fragments arranged by size:
720 237 54
AlwNI CAG_nnn'CTG
Cuts at: 0 138 1011
Size: 138 873
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 1011
Size: 36 975
BbvCI CC'TCA_GC
Cuts at: 0 98 303 1011
Size: 98 205 708
Fragments arranged by size:
708 205 98
BglI GCCn_nnn'nGGC
Cuts at: 0 61 1011
Size: 61 950
BlpI GC'TnA_GC
Cuts at: 0 350 1011
Size: 350 661
BmrI ACTGGGnnnn_n'
Cuts at: 0 546 1011
Size: 546 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 597 1011
Size: 410 187 414
Fragments arranged by size:
414 410 187
Bpu10I CC'TnA_GC
Cuts at: 0 98 160 303 1011
Size: 98 62 143 708
Fragments arranged by size:
708 143 98 62
BsaHI Gr'CG_yC
Cuts at: 0 944 1011
Size: 944 67
BsaWI w'CCGG_w
Cuts at: 0 975 1011
Size: 975 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 468 1011
Size: 86 382 543
Fragments arranged by size:
543 382 86
BseYI C'CCAG_C
Cuts at: 0 83 354 1011
Size: 83 271 657
Fragments arranged by size:
657 271 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1011
Size: 214 797
BsiEI CG_ry'CG
Cuts at: 0 7 230 1011
Size: 7 223 781
Fragments arranged by size:
781 223 7
BsiHKAI G_wGCw'C
Cuts at: 0 363 1011
Size: 363 648
Bsp1286I G_dGCh'C
Cuts at: 0 363 1011
Size: 363 648
BspEI T'CCGG_A
Cuts at: 0 975 1011
Size: 975 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 1011
Size: 123 888
BsrBI CCG'CTC
Cuts at: 0 94 1011
Size: 94 917
BsrGI T'GTAC_A
Cuts at: 0 119 256 1011
Size: 119 137 755
Fragments arranged by size:
755 137 119
BssSI C'ACGA_G
Cuts at: 0 171 1011
Size: 171 840
BstEII G'GTnAC_C
Cuts at: 0 441 1011
Size: 441 570
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 1011
Size: 594 417
BtgI C'CryG_G
Cuts at: 0 59 506 755 1011
Size: 59 447 249 256
Fragments arranged by size:
447 256 249 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 477 1011
Size: 477 534
BtsI GCAGTG_nn'
Cuts at: 0 287 1011
Size: 287 724
DraI TTT'AAA
Cuts at: 0 201 884 1011
Size: 201 683 127
Fragments arranged by size:
683 201 127
EaeI y'GGCC_r
Cuts at: 0 227 1011
Size: 227 784
EagI C'GGCC_G
Cuts at: 0 227 1011
Size: 227 784
EarI CTCTTCn'nnn_
Cuts at: 0 869 1011
Size: 869 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 597 718 1011
Size: 410 187 121 293
Fragments arranged by size:
410 293 187 121
EcoO109I rG'GnC_Cy
Cuts at: 0 135 568 1011
Size: 135 433 443
Fragments arranged by size:
443 433 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 943 975 1011
Size: 223 32 688 32 36
Fragments arranged by size:
688 223 36 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1011
Size: 579 432
MslI CAynn'nnrTG
Cuts at: 0 247 439 1011
Size: 247 192 572
Fragments arranged by size:
572 247 192
MspA1I CmG'CkG
Cuts at: 0 61 87 1011
Size: 61 26 924
Fragments arranged by size:
924 61 26
PpuMI rG'GwC_Cy
Cuts at: 0 135 1011
Size: 135 876
PvuII CAG'CTG
Cuts at: 0 87 1011
Size: 87 924
SacII CC_GC'GG
Cuts at: 0 62 1011
Size: 62 949
StuI AGG'CCT
Cuts at: 0 177 824 913 1011
Size: 177 647 89 98
Fragments arranged by size:
647 177 98 89
StyI C'CwwG_G
Cuts at: 0 388 914 1011
Size: 388 526 97
Fragments arranged by size:
526 388 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 366 1011
Size: 21 345 645
Fragments arranged by size:
645 345 21
TatI w'GTAC_w
Cuts at: 0 119 256 620 1011
Size: 119 137 364 391
Fragments arranged by size:
391 364 137 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 842 874 1011
Size: 842 32 137
Fragments arranged by size:
842 137 32
XmnI GAAnn'nnTTC
Cuts at: 0 765 995 1011
Size: 765 230 16
Fragments arranged by size:
765 230 16
ZraI GAC'GTC
Cuts at: 0 945 1011
Size: 945 66
Enzymes that do cut:
AatII AccI AcuI AhdI AlwNI BbsI BbvCI BglI
BlpI BmrI BpmI Bpu10I BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrGI
BssSI BstEII BstXI BtgI BtgZI BtsI DraI EaeI
EagI EarI Eco57MI EcoO109I Hin4I MmeI MslI MspA1I
PpuMI PvuII SacII StuI StyI TaqII TatI TstI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BfrBI BglII Bme1580I BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI
BspHI BsrDI BsrFI BssHII BstAPI BstBI BstYI BstZ17I
Bsu36I ClaI CspCI DraIII DrdI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI