Restriction map for
pF1KB6483
(Linear) (Six-base) MAPSORT of: KIBB6483.seq Check: 4148 from: 1 to: 984
KIBB6483.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 860 984
Size: 860 124
AflIII A'CryG_T
Cuts at: 0 368 920 984
Size: 368 552 64
Fragments arranged by size:
552 368 64
AlwNI CAG_nnn'CTG
Cuts at: 0 834 984
Size: 834 150
ApaI G_GGCC'C
Cuts at: 0 54 984
Size: 54 930
ApaLI G'TGCA_C
Cuts at: 0 100 984
Size: 100 884
ApoI r'AATT_y
Cuts at: 0 199 451 984
Size: 199 252 533
Fragments arranged by size:
533 252 199
AvaI C'yCGr_G
Cuts at: 0 497 551 580 984
Size: 497 54 29 404
Fragments arranged by size:
497 404 54 29
BanI G'GyrC_C
Cuts at: 0 776 968 984
Size: 776 192 16
Fragments arranged by size:
776 192 16
BanII G_rGCy'C
Cuts at: 0 54 984
Size: 54 930
BglI GCCn_nnn'nGGC
Cuts at: 0 86 984
Size: 86 898
BlpI GC'TnA_GC
Cuts at: 0 889 984
Size: 889 95
Bme1580I G_kGCm'C
Cuts at: 0 54 104 779 984
Size: 54 50 675 205
Fragments arranged by size:
675 205 54 50
BmgBI CAC'GTC
Cuts at: 0 462 984
Size: 462 522
BmrI ACTGGGnnnn_n'
Cuts at: 0 813 984
Size: 813 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 143 984
Size: 143 841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 984
Size: 646 338
Bpu10I CC'TnA_GC
Cuts at: 0 118 984
Size: 118 866
BsaI GGTCTCn'nnnn_
Cuts at: 0 469 984
Size: 469 515
BsaAI yAC'GTr
Cuts at: 0 371 984
Size: 371 613
BsaHI Gr'CG_yC
Cuts at: 0 140 984
Size: 140 844
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 703 733 984
Size: 703 30 251
Fragments arranged by size:
703 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 571 598 682 721 724 984
Size: 421 150 27 84 39 3 260
Fragments arranged by size:
421 260 150 84 39 27 3
BseYI C'CCAG_C
Cuts at: 0 284 984
Size: 284 700
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 745 841 984
Size: 559 186 96 143
Fragments arranged by size:
559 186 143 96
BsiHKAI G_wGCw'C
Cuts at: 0 104 984
Size: 104 880
BsiWI C'GTAC_G
Cuts at: 0 371 984
Size: 371 613
Bsp1286I G_dGCh'C
Cuts at: 0 54 104 779 984
Size: 54 50 675 205
Fragments arranged by size:
675 205 54 50
BspHI T'CATG_A
Cuts at: 0 456 984
Size: 456 528
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 860 984
Size: 860 124
BsrBI CCG'CTC
Cuts at: 0 507 984
Size: 507 477
BsrFI r'CCGG_y
Cuts at: 0 714 984
Size: 714 270
BssSI C'ACGA_G
Cuts at: 0 859 984
Size: 859 125
BstYI r'GATC_y
Cuts at: 0 681 759 984
Size: 681 78 225
Fragments arranged by size:
681 225 78
BtgI C'CryG_G
Cuts at: 0 144 984
Size: 144 840
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 660 984
Size: 660 324
DraIII CAC_nnn'GTG
Cuts at: 0 807 984
Size: 807 177
EaeI y'GGCC_r
Cuts at: 0 87 191 296 429 521 545 984
Size: 87 104 105 133 92 24 439
Fragments arranged by size:
439 133 105 104 92 87 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 258 984
Size: 258 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 984
Size: 646 338
EcoO109I rG'GnC_Cy
Cuts at: 0 50 79 630 957 984
Size: 50 29 551 327 27
Fragments arranged by size:
551 327 50 29 27
EcoRI G'AATT_C
Cuts at: 0 199 984
Size: 199 785
FspI TGC'GCA
Cuts at: 0 415 984
Size: 415 569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 512 523 534 555 566 673 705
Size: 480 32 11 11 21 11 107 32
Cuts at: 705 984
Size: 279
Fragments arranged by size:
480 279 107 32 32 21 11 11
11
MscI TGG'CCA
Cuts at: 0 89 193 431 547 984
Size: 89 104 238 116 437
Fragments arranged by size:
437 238 116 104 89
MslI CAynn'nnrTG
Cuts at: 0 782 925 974 984
Size: 782 143 49 10
Fragments arranged by size:
782 143 49 10
MspA1I CmG'CkG
Cuts at: 0 333 984
Size: 333 651
NaeI GCC'GGC
Cuts at: 0 716 984
Size: 716 268
NgoMIV G'CCGG_C
Cuts at: 0 714 984
Size: 714 270
NspI r_CATG'y
Cuts at: 0 600 924 984
Size: 600 324 60
Fragments arranged by size:
600 324 60
PasI CC'CwG_GG
Cuts at: 0 54 984
Size: 54 930
PciI A'CATG_T
Cuts at: 0 920 984
Size: 920 64
PflMI CCAn_nnn'nTGG
Cuts at: 0 834 984
Size: 834 150
PfoI T'CCnGG_A
Cuts at: 0 746 755 984
Size: 746 9 229
Fragments arranged by size:
746 229 9
PpuMI rG'GwC_Cy
Cuts at: 0 630 957 984
Size: 630 327 27
Fragments arranged by size:
630 327 27
PspOMI G'GGCC_C
Cuts at: 0 50 984
Size: 50 934
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 142 174 984
Size: 142 32 810
Fragments arranged by size:
810 142 32
RsrII CG'GwC_CG
Cuts at: 0 381 984
Size: 381 603
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 86 984
Size: 86 898
SmlI C'TyrA_G
Cuts at: 0 551 984
Size: 551 433
StyI C'CwwG_G
Cuts at: 0 194 984
Size: 194 790
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 276 711 795 984
Size: 276 435 84 189
Fragments arranged by size:
435 276 189 84
TatI w'GTAC_w
Cuts at: 0 671 686 984
Size: 671 15 298
Fragments arranged by size:
671 298 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 703 735 984
Size: 173 32 498 32 249
Fragments arranged by size:
498 249 173 32 32
XhoI C'TCGA_G
Cuts at: 0 551 984
Size: 551 433
XmnI GAAnn'nnTTC
Cuts at: 0 452 984
Size: 452 532
Enzymes that do cut:
AarI AflIII AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrBI BsrFI BssSI
BstYI BtgI BtgZI DraIII EaeI EciI Eco57MI EcoO109I
EcoRI FspI Hin4I MscI MslI MspA1I NaeI NgoMIV
NspI PasI PciI PflMI PfoI PpuMI PspOMI PsrI
RsrII SfiI SmlI StyI TaqII TatI TstI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmtI BpuEI BsaBI BsaWI BsiEI BsmI
BsmBI BspEI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EarI EcoICRI EcoNI EcoRV FalI FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XmaI ZraI