(Linear) (Six-base) MAPSORT of: KIBB6484.seq  Check: 9818  from: 1  to: 1068



KIBB6484.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0     469     963    1068 
   Size:        469     494     105

  Fragments arranged by size: 

                 494     469     105
 
AflIII A'CryG_T

Cuts at:      0     581    1068 
   Size:        581     487
 
AlwNI CAG_nnn'CTG

Cuts at:      0     136    1068 
   Size:        136     932
 
ApoI r'AATT_y

Cuts at:      0    1012    1068 
   Size:       1012      56
 
BlpI GC'TnA_GC

Cuts at:      0     431    1068 
   Size:        431     637
 
BsaAI yAC'GTr

Cuts at:      0     169     582    1068 
   Size:        169     413     486

  Fragments arranged by size: 

                 486     413     169
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     794     824    1068 
   Size:        794      30     244

  Fragments arranged by size: 

                 794     244      30
 
BseYI C'CCAG_C

Cuts at:      0      71     559     969    1068 
   Size:         71     488     410      99

  Fragments arranged by size: 

                 488     410      99      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109    1068 
   Size:        109     959
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     481    1068 
   Size:        481     587
 
BsrDI GCAATG_nn'

Cuts at:      0     257    1068 
   Size:        257     811
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     441    1068 
   Size:        441     627
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1017    1068 
   Size:       1017      51
 
BtgI C'CryG_G

Cuts at:      0     452    1068 
   Size:        452     616
 
BtsI GCAGTG_nn'

Cuts at:      0      97    1068 
   Size:         97     971
 
DraI TTT'AAA

Cuts at:      0     387    1068 
   Size:        387     681
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     316    1068 
   Size:        316     752
 
EaeI y'GGCC_r

Cuts at:      0    1007    1068 
   Size:       1007      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     879    1068 
   Size:        879     189
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     697    1068 
   Size:        697     371
 
HincII GTy'rAC

Cuts at:      0     411    1068 
   Size:        411     657
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     165    1068 
   Size:        165     903
 
MscI TGG'CCA

Cuts at:      0    1009    1068 
   Size:       1009      59
 
MslI CAynn'nnrTG

Cuts at:      0     905    1068 
   Size:        905     163
 
MspA1I CmG'CkG

Cuts at:      0      75     969    1068 
   Size:         75     894      99

  Fragments arranged by size: 

                 894      99      75
 
NcoI C'CATG_G

Cuts at:      0     452    1068 
   Size:        452     616
 
NspI r_CATG'y

Cuts at:      0     439    1068 
   Size:        439     629
 
PmlI CAC'GTG

Cuts at:      0     582    1068 
   Size:        582     486
 
PsiI TTA'TAA

Cuts at:      0    1028    1068 
   Size:       1028      40
 
PstI C_TGCA'G

Cuts at:      0     478     700     726    1068 
   Size:        478     222      26     342

  Fragments arranged by size: 

                 478     342     222      26
 
PvuII CAG'CTG

Cuts at:      0      75     969    1068 
   Size:         75     894      99

  Fragments arranged by size: 

                 894      99      75
 
SexAI A'CCwGG_T

Cuts at:      0      83    1068 
   Size:         83     985
 
SfcI C'TryA_G

Cuts at:      0     474     696     722     964    1068 
   Size:        474     222      26     242     104

  Fragments arranged by size: 

                 474     242     222     104      26
 
SphI G_CATG'C

Cuts at:      0     439    1068 
   Size:        439     629
 
StyI C'CwwG_G

Cuts at:      0      35     452    1068 
   Size:         35     417     616

  Fragments arranged by size: 

                 616     417      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     326    1068 
   Size:        326     742
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     794     826    1068 
   Size:        794      32     242

  Fragments arranged by size: 

                 794     242      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     990    1068 
   Size:        990      78
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApoI     BlpI    BsaAI    BsaXI    BseYI
     BsgI    BspMI    BsrDI   BstAPI    BstXI     BtgI     BtsI     DraI
     DrdI     EaeI EcoO109I     FalI   HincII     MmeI     MscI     MslI
   MspA1I     NcoI     NspI     PmlI     PsiI     PstI    PvuII    SexAI
     SfcI     SphI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI