Restriction map for
pF1KB6485
(Linear) (Six-base) MAPSORT of: KIBB6485.seq Check: 5835 from: 1 to: 984
KIBB6485.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 564 984
Size: 564 420
AccI GT'mk_AC
Cuts at: 0 926 984
Size: 926 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 433 572 721 984
Size: 244 189 139 149 263
Fragments arranged by size:
263 244 189 149 139
AleI CACnn'nnGTG
Cuts at: 0 628 984
Size: 628 356
AlwNI CAG_nnn'CTG
Cuts at: 0 534 595 984
Size: 534 61 389
Fragments arranged by size:
534 389 61
AvaI C'yCGr_G
Cuts at: 0 692 713 755 984
Size: 692 21 42 229
Fragments arranged by size:
692 229 42 21
BamHI G'GATC_C
Cuts at: 0 497 984
Size: 497 487
BanII G_rGCy'C
Cuts at: 0 554 984
Size: 554 430
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 709 984
Size: 232 477 275
Fragments arranged by size:
477 275 232
BclI T'GATC_A
Cuts at: 0 975 984
Size: 975 9
BfrBI ATG'CAT
Cuts at: 0 514 984
Size: 514 470
Bme1580I G_kGCm'C
Cuts at: 0 28 815 984
Size: 28 787 169
Fragments arranged by size:
787 169 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 424 822 984
Size: 317 107 398 162
Fragments arranged by size:
398 317 162 107
Bpu10I CC'TnA_GC
Cuts at: 0 10 984
Size: 10 974
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 984
Size: 292 692
BsaWI w'CCGG_w
Cuts at: 0 491 984
Size: 491 493
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 427 484 984
Size: 55 372 57 500
Fragments arranged by size:
500 372 57 55
BseYI C'CCAG_C
Cuts at: 0 569 814 984
Size: 569 245 170
Fragments arranged by size:
569 245 170
BsiEI CG_ry'CG
Cuts at: 0 867 984
Size: 867 117
BsiHKAI G_wGCw'C
Cuts at: 0 11 554 984
Size: 11 543 430
Fragments arranged by size:
543 430 11
Bsp1286I G_dGCh'C
Cuts at: 0 11 28 554 815 984
Size: 11 17 526 261 169
Fragments arranged by size:
526 261 169 17 11
BspEI T'CCGG_A
Cuts at: 0 491 984
Size: 491 493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 564 792 984
Size: 564 228 192
Fragments arranged by size:
564 228 192
BsrFI r'CCGG_y
Cuts at: 0 866 984
Size: 866 118
BstEII G'GTnAC_C
Cuts at: 0 738 984
Size: 738 246
BstYI r'GATC_y
Cuts at: 0 497 984
Size: 497 487
Bsu36I CC'TnA_GG
Cuts at: 0 157 984
Size: 157 827
BtgI C'CryG_G
Cuts at: 0 375 984
Size: 375 609
BtsI GCAGTG_nn'
Cuts at: 0 233 623 737 984
Size: 233 390 114 247
Fragments arranged by size:
390 247 233 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 237 272 984
Size: 237 35 712
Fragments arranged by size:
712 237 35
EaeI y'GGCC_r
Cuts at: 0 864 884 984
Size: 864 20 100
Fragments arranged by size:
864 100 20
EagI C'GGCC_G
Cuts at: 0 864 984
Size: 864 120
EarI CTCTTCn'nnn_
Cuts at: 0 104 984
Size: 104 880
EcoICRI GAG'CTC
Cuts at: 0 552 984
Size: 552 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 317 424 433 572 721 822 984
Size: 244 73 107 9 139 149 101 162
Fragments arranged by size:
244 162 149 139 107 101 73 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 109 984
Size: 37 72 875
Fragments arranged by size:
875 72 37
EcoO109I rG'GnC_Cy
Cuts at: 0 144 717 935 944 984
Size: 144 573 218 9 40
Fragments arranged by size:
573 218 144 40 9
HaeII r_GCGC'y
Cuts at: 0 206 984
Size: 206 778
MscI TGG'CCA
Cuts at: 0 886 984
Size: 886 98
MslI CAynn'nnrTG
Cuts at: 0 628 730 984
Size: 628 102 254
Fragments arranged by size:
628 254 102
MspA1I CmG'CkG
Cuts at: 0 444 687 770 984
Size: 444 243 83 214
Fragments arranged by size:
444 243 214 83
NaeI GCC'GGC
Cuts at: 0 868 984
Size: 868 116
NcoI C'CATG_G
Cuts at: 0 375 984
Size: 375 609
NgoMIV G'CCGG_C
Cuts at: 0 866 984
Size: 866 118
NsiI A_TGCA'T
Cuts at: 0 516 984
Size: 516 468
NspI r_CATG'y
Cuts at: 0 514 984
Size: 514 470
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 921 984
Size: 534 387 63
Fragments arranged by size:
534 387 63
PfoI T'CCnGG_A
Cuts at: 0 799 830 914 984
Size: 799 31 84 70
Fragments arranged by size:
799 84 70 31
PpuMI rG'GwC_Cy
Cuts at: 0 717 935 944 984
Size: 717 218 9 40
Fragments arranged by size:
717 218 40 9
PstI C_TGCA'G
Cuts at: 0 102 386 548 984
Size: 102 284 162 436
Fragments arranged by size:
436 284 162 102
PvuII CAG'CTG
Cuts at: 0 444 687 984
Size: 444 243 297
Fragments arranged by size:
444 297 243
SacI G_AGCT'C
Cuts at: 0 554 984
Size: 554 430
SexAI A'CCwGG_T
Cuts at: 0 89 984
Size: 89 895
SfcI C'TryA_G
Cuts at: 0 98 382 544 984
Size: 98 284 162 440
Fragments arranged by size:
440 284 162 98
SmaI CCC'GGG
Cuts at: 0 715 984
Size: 715 269
SmlI C'TyrA_G
Cuts at: 0 755 984
Size: 755 229
SphI G_CATG'C
Cuts at: 0 514 984
Size: 514 470
StyI C'CwwG_G
Cuts at: 0 375 720 984
Size: 375 345 264
Fragments arranged by size:
375 345 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 984
Size: 609 375
Tth111I GACn'n_nGTC
Cuts at: 0 619 984
Size: 619 365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 577 984
Size: 577 407
XhoI C'TCGA_G
Cuts at: 0 755 984
Size: 755 229
XmaI C'CCGG_G
Cuts at: 0 713 984
Size: 713 271
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI AvaI BamHI BanII
BbsI BclI BfrBI Bme1580I BpmI Bpu10I BsaI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrFI
BstEII BstYI Bsu36I BtgI BtsI CspCI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII MscI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PflMI PfoI
PpuMI PstI PvuII SacI SexAI SfcI SmaI SmlI
SphI StyI TaqII Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaXI BsgI BsiWI BsmI BsmBI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgZI ClaI DraI DraIII DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TatI TstI XbaI XmnI ZraI