Restriction map for
pF1KB6488
(Linear) (Six-base) MAPSORT of: KIBB6488.seq Check: 6807 from: 1 to: 1071
KIBB6488.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 403 833 1071
Size: 403 430 238
Fragments arranged by size:
430 403 238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1071
Size: 1069 2
AfeI AGC'GCT
Cuts at: 0 100 1071
Size: 100 971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 865 1071
Size: 865 206
AlwNI CAG_nnn'CTG
Cuts at: 0 872 1041 1071
Size: 872 169 30
Fragments arranged by size:
872 169 30
ApaLI G'TGCA_C
Cuts at: 0 390 1071
Size: 390 681
ApoI r'AATT_y
Cuts at: 0 364 382 1071
Size: 364 18 689
Fragments arranged by size:
689 364 18
AvaI C'yCGr_G
Cuts at: 0 117 154 677 1071
Size: 117 37 523 394
Fragments arranged by size:
523 394 117 37
AvrII C'CTAG_G
Cuts at: 0 582 1071
Size: 582 489
BanI G'GyrC_C
Cuts at: 0 486 1071
Size: 486 585
BbsI GAAGACnn'nnnn_
Cuts at: 0 1057 1071
Size: 1057 14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 23 57 1071
Size: 23 34 1014
Fragments arranged by size:
1014 34 23
BglI GCCn_nnn'nGGC
Cuts at: 0 212 1071
Size: 212 859
BglII A'GATC_T
Cuts at: 0 228 1071
Size: 228 843
Bme1580I G_kGCm'C
Cuts at: 0 118 130 204 394 425 1071
Size: 118 12 74 190 31 646
Fragments arranged by size:
646 190 118 74 31 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 105 550 1071
Size: 105 445 521
Fragments arranged by size:
521 445 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 240 1071
Size: 240 831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 512 1018 1063 1071
Size: 253 259 506 45 8
Fragments arranged by size:
506 259 253 45 8
Bpu10I CC'TnA_GC
Cuts at: 0 452 1071
Size: 452 619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 1071
Size: 588 483
BsaHI Gr'CG_yC
Cuts at: 0 136 1071
Size: 136 935
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 578 608 1071
Size: 578 30 463
Fragments arranged by size:
578 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 1071
Size: 544 527
BseYI C'CCAG_C
Cuts at: 0 283 465 1071
Size: 283 182 606
Fragments arranged by size:
606 283 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 288 825 835 885 975 1071
Size: 31 257 537 10 50 90 96
Fragments arranged by size:
537 257 96 90 50 31 10
BsiHKAI G_wGCw'C
Cuts at: 0 19 394 946 1071
Size: 19 375 552 125
Fragments arranged by size:
552 375 125 19
Bsp1286I G_dGCh'C
Cuts at: 0 19 118 130 204 394 425 946 1071
Size: 19 99 12 74 190 31 521 125
Fragments arranged by size:
521 190 125 99 74 31 19 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 154 403 833 997 1071
Size: 154 249 430 164 74
Fragments arranged by size:
430 249 164 154 74
BssHII G'CGCG_C
Cuts at: 0 162 1071
Size: 162 909
BssSI C'ACGA_G
Cuts at: 0 18 1071
Size: 18 1053
BstAPI GCAn_nnn'nTGC
Cuts at: 0 9 1071
Size: 9 1062
BstEII G'GTnAC_C
Cuts at: 0 681 1071
Size: 681 390
BstYI r'GATC_y
Cuts at: 0 228 512 1071
Size: 228 284 559
Fragments arranged by size:
559 284 228
BtsI GCAGTG_nn'
Cuts at: 0 46 258 360 891 1071
Size: 46 212 102 531 180
Fragments arranged by size:
531 212 180 102 46
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 639 674 1071
Size: 639 35 397
Fragments arranged by size:
639 397 35
DraIII CAC_nnn'GTG
Cuts at: 0 635 1071
Size: 635 436
DrdI GACnn_nn'nnGTC
Cuts at: 0 607 1071
Size: 607 464
EaeI y'GGCC_r
Cuts at: 0 891 1071
Size: 891 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 512 1018 1063 1069 1071
Size: 253 259 506 45 6 2
Fragments arranged by size:
506 259 253 45 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 1071
Size: 79 992
EcoO109I rG'GnC_Cy
Cuts at: 0 986 1071
Size: 986 85
HaeII r_GCGC'y
Cuts at: 0 102 1071
Size: 102 969
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 166 198 578 610 1071
Size: 12 32 122 32 380 32 461
Fragments arranged by size:
461 380 122 32 32 32 12
HincII GTy'rAC
Cuts at: 0 612 1071
Size: 612 459
MspA1I CmG'CkG
Cuts at: 0 428 435 896 1071
Size: 428 7 461 175
Fragments arranged by size:
461 428 175 7
NspI r_CATG'y
Cuts at: 0 647 1071
Size: 647 424
PasI CC'CwG_GG
Cuts at: 0 949 1071
Size: 949 122
PflMI CCAn_nnn'nTGG
Cuts at: 0 75 635 1041 1071
Size: 75 560 406 30
Fragments arranged by size:
560 406 75 30
PfoI T'CCnGG_A
Cuts at: 0 1034 1071
Size: 1034 37
PpuMI rG'GwC_Cy
Cuts at: 0 986 1071
Size: 986 85
PstI C_TGCA'G
Cuts at: 0 400 830 886 1071
Size: 400 430 56 185
Fragments arranged by size:
430 400 185 56
PvuII CAG'CTG
Cuts at: 0 435 1071
Size: 435 636
SfcI C'TryA_G
Cuts at: 0 396 826 882 1071
Size: 396 430 56 189
Fragments arranged by size:
430 396 189 56
SmaI CCC'GGG
Cuts at: 0 119 156 679 1071
Size: 119 37 523 392
Fragments arranged by size:
523 392 119 37
SmlI C'TyrA_G
Cuts at: 0 603 1071
Size: 603 468
SphI G_CATG'C
Cuts at: 0 647 1071
Size: 647 424
SrfI GCCC'GGGC
Cuts at: 0 119 1071
Size: 119 952
StuI AGG'CCT
Cuts at: 0 548 1027 1071
Size: 548 479 44
Fragments arranged by size:
548 479 44
StyI C'CwwG_G
Cuts at: 0 582 1071
Size: 582 489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 993 1025 1071
Size: 993 32 46
Fragments arranged by size:
993 46 32
XmaI C'CCGG_G
Cuts at: 0 117 154 677 1071
Size: 117 37 523 394
Fragments arranged by size:
523 394 117 37
Enzymes that do cut:
AarI AcuI AfeI AlfI AlwNI ApaLI ApoI AvaI
AvrII BanI BbsI BcgI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BssHII BssSI BstAPI BstEII
BstYI BtsI CspCI DraIII DrdI EaeI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MspA1I NspI PasI PflMI
PfoI PpuMI PstI PvuII SfcI SmaI SmlI SphI
SrfI StuI StyI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI BaeI BamHI
BanII BbeI BbvCI BciVI BclI BfrBI BlpI BmgBI
BmtI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstBI
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SspI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmnI ZraI