Restriction map for
pF1KB6492
(Linear) (Six-base) MAPSORT of: KIBB6492.seq Check: 1258 from: 1 to: 1071
KIBB6492.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 384 1071
Size: 384 687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 691 1071
Size: 268 423 380
Fragments arranged by size:
423 380 268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 1071
Size: 664 407
AlwNI CAG_nnn'CTG
Cuts at: 0 204 702 1032 1071
Size: 204 498 330 39
Fragments arranged by size:
498 330 204 39
ApaI G_GGCC'C
Cuts at: 0 74 1071
Size: 74 997
ApoI r'AATT_y
Cuts at: 0 487 1071
Size: 487 584
AvaI C'yCGr_G
Cuts at: 0 67 1071
Size: 67 1004
BanI G'GyrC_C
Cuts at: 0 3 418 1071
Size: 3 415 653
Fragments arranged by size:
653 415 3
BanII G_rGCy'C
Cuts at: 0 38 74 236 764 842 1056 1071
Size: 38 36 162 528 78 214 15
Fragments arranged by size:
528 214 162 78 38 36 15
BbeI G_GCGC'C
Cuts at: 0 7 1071
Size: 7 1064
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 481 1071
Size: 434 47 590
Fragments arranged by size:
590 434 47
BbvCI CC'TCA_GC
Cuts at: 0 913 1071
Size: 913 158
BglI GCCn_nnn'nGGC
Cuts at: 0 95 731 1071
Size: 95 636 340
Fragments arranged by size:
636 340 95
Bme1580I G_kGCm'C
Cuts at: 0 74 1071
Size: 74 997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 841 913 1000 1032 1071
Size: 589 252 72 87 32 39
Fragments arranged by size:
589 252 87 72 39 32
Bpu10I CC'TnA_GC
Cuts at: 0 577 625 913 1071
Size: 577 48 288 158
Fragments arranged by size:
577 288 158 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1071
Size: 826 245
BsaI GGTCTCn'nnnn_
Cuts at: 0 565 1071
Size: 565 506
BsaHI Gr'CG_yC
Cuts at: 0 4 1071
Size: 4 1067
BsaWI w'CCGG_w
Cuts at: 0 148 1071
Size: 148 923
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 772 850 1071
Size: 169 603 78 221
Fragments arranged by size:
603 221 169 78
BseYI C'CCAG_C
Cuts at: 0 197 999 1071
Size: 197 802 72
Fragments arranged by size:
802 197 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1071
Size: 634 437
BsiHKAI G_wGCw'C
Cuts at: 0 236 842 1056 1071
Size: 236 606 214 15
Fragments arranged by size:
606 236 214 15
Bsp1286I G_dGCh'C
Cuts at: 0 38 74 236 764 842 1056 1071
Size: 38 36 162 528 78 214 15
Fragments arranged by size:
528 214 162 78 38 36 15
BspEI T'CCGG_A
Cuts at: 0 148 1071
Size: 148 923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 384 1071
Size: 384 687
BssSI C'ACGA_G
Cuts at: 0 550 598 904 1071
Size: 550 48 306 167
Fragments arranged by size:
550 306 167 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 975 1071
Size: 975 96
BstBI TT'CG_AA
Cuts at: 0 851 1071
Size: 851 220
BstEII G'GTnAC_C
Cuts at: 0 396 1071
Size: 396 675
BstXI CCAn_nnnn'nTGG
Cuts at: 0 999 1071
Size: 999 72
BstYI r'GATC_y
Cuts at: 0 1043 1071
Size: 1043 28
BtgI C'CryG_G
Cuts at: 0 7 90 1071
Size: 7 83 981
Fragments arranged by size:
981 83 7
EaeI y'GGCC_r
Cuts at: 0 227 356 1071
Size: 227 129 715
Fragments arranged by size:
715 227 129
EarI CTCTTCn'nnn_
Cuts at: 0 67 1071
Size: 67 1004
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 786 1002 1071
Size: 786 216 69
Fragments arranged by size:
786 216 69
EcoICRI GAG'CTC
Cuts at: 0 234 840 1054 1071
Size: 234 606 214 17
Fragments arranged by size:
606 234 214 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 589 691 841 913 1000 1032 1071
Size: 268 321 102 150 72 87 32 39
Fragments arranged by size:
321 268 150 102 87 72 39 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 979 1071
Size: 979 92
EcoO109I rG'GnC_Cy
Cuts at: 0 71 699 783 1029 1071
Size: 71 628 84 246 42
Fragments arranged by size:
628 246 84 71 42
FspI TGC'GCA
Cuts at: 0 685 715 902 1071
Size: 685 30 187 169
Fragments arranged by size:
685 187 169 30
HaeII r_GCGC'y
Cuts at: 0 7 46 1071
Size: 7 39 1025
Fragments arranged by size:
1025 39 7
HindIII A'AGCT_T
Cuts at: 0 289 1071
Size: 289 782
KasI G'GCGC_C
Cuts at: 0 3 1071
Size: 3 1068
MscI TGG'CCA
Cuts at: 0 358 1071
Size: 358 713
MspA1I CmG'CkG
Cuts at: 0 9 82 183 891 1071
Size: 9 73 101 708 180
Fragments arranged by size:
708 180 101 73 9
NarI GG'CG_CC
Cuts at: 0 4 1071
Size: 4 1067
PasI CC'CwG_GG
Cuts at: 0 764 1071
Size: 764 307
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 1071
Size: 702 369
PfoI T'CCnGG_A
Cuts at: 0 830 1071
Size: 830 241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 1071
Size: 736 32 303
Fragments arranged by size:
736 303 32
PpuMI rG'GwC_Cy
Cuts at: 0 783 1029 1071
Size: 783 246 42
Fragments arranged by size:
783 246 42
PspOMI G'GGCC_C
Cuts at: 0 70 1071
Size: 70 1001
PstI C_TGCA'G
Cuts at: 0 1028 1071
Size: 1028 43
PvuII CAG'CTG
Cuts at: 0 183 891 1071
Size: 183 708 180
Fragments arranged by size:
708 183 180
SacI G_AGCT'C
Cuts at: 0 236 842 1056 1071
Size: 236 606 214 15
Fragments arranged by size:
606 236 214 15
SacII CC_GC'GG
Cuts at: 0 10 1071
Size: 10 1061
SapI GCTCTTCn'nnn_
Cuts at: 0 67 1071
Size: 67 1004
ScaI AGT'ACT
Cuts at: 0 103 1071
Size: 103 968
SfcI C'TryA_G
Cuts at: 0 735 1024 1071
Size: 735 289 47
Fragments arranged by size:
735 289 47
SfoI GGC'GCC
Cuts at: 0 5 1071
Size: 5 1066
SmlI C'TyrA_G
Cuts at: 0 841 1071
Size: 841 230
StyI C'CwwG_G
Cuts at: 0 992 1071
Size: 992 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 436 601 1071
Size: 436 165 470
Fragments arranged by size:
470 436 165
TatI w'GTAC_w
Cuts at: 0 101 554 1071
Size: 101 453 517
Fragments arranged by size:
517 453 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 445 477 1071
Size: 445 32 594
Fragments arranged by size:
594 445 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 1071
Size: 366 705
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII HindIII KasI MscI MspA1I NarI
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SapI ScaI SfcI SfoI SmlI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaAI BsaBI BsaXI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HpaI KpnI MfeI MluI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI