(Linear) (Six-base) MAPSORT of: KIBB6493.seq  Check: 5610  from: 1  to: 993



KIBB6493.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     375     993 
   Size:        375     618
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     943     968     993 
   Size:        943      25      25

  Fragments arranged by size: 

                 943      25      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     559     993 
   Size:        559     434
 
ApoI r'AATT_y

Cuts at:      0     505     630     993 
   Size:        505     125     363

  Fragments arranged by size: 

                 505     363     125
 
AseI AT'TA_AT

Cuts at:      0     332     485     993 
   Size:        332     153     508

  Fragments arranged by size: 

                 508     332     153
 
BamHI G'GATC_C

Cuts at:      0     858     993 
   Size:        858     135
 
BanII G_rGCy'C

Cuts at:      0     686     993 
   Size:        686     307
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     765     993 
   Size:        765     228
 
BglII A'GATC_T

Cuts at:      0     578     993 
   Size:        578     415
 
Bme1580I G_kGCm'C

Cuts at:      0       7     993 
   Size:          7     986
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     993 
   Size:        422     571
 
Bpu10I CC'TnA_GC

Cuts at:      0     980     993 
   Size:        980      13
 
BsaBI GATnn'nnATC

Cuts at:      0     278     993 
   Size:        278     715
 
BsaWI w'CCGG_w

Cuts at:      0      42     993 
   Size:         42     951
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     839     869     993 
   Size:        839      30     124

  Fragments arranged by size: 

                 839     124      30
 
BseYI C'CCAG_C

Cuts at:      0     571     993 
   Size:        571     422
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     686     993 
   Size:          7     679     307

  Fragments arranged by size: 

                 679     307       7
 
BstYI r'GATC_y

Cuts at:      0     171     578     858     993 
   Size:        171     407     280     135

  Fragments arranged by size: 

                 407     280     171     135
 
Bsu36I CC'TnA_GG

Cuts at:      0     675     993 
   Size:        675     318
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     175     993 
   Size:        175     818
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     993 
   Size:        200     793
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422     943     968     993 
   Size:        422     521      25      25

  Fragments arranged by size: 

                 521     422      25      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     732     993 
   Size:        732     261
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     452     973     993 
   Size:        452     521      20

  Fragments arranged by size: 

                 521     452      20
 
HindIII A'AGCT_T

Cuts at:      0     472     993 
   Size:        472     521
 
MslI CAynn'nnrTG

Cuts at:      0     242     993 
   Size:        242     751
 
NspI r_CATG'y

Cuts at:      0      70     809     945     993 
   Size:         70     739     136      48

  Fragments arranged by size: 

                 739     136      70      48
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     956     988     993 
   Size:        956      32       5

  Fragments arranged by size: 

                 956      32       5
 
PpuMI rG'GwC_Cy

Cuts at:      0     732     993 
   Size:        732     261
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     653     685     993 
   Size:        653      32     308

  Fragments arranged by size: 

                 653     308      32
 
SexAI A'CCwGG_T

Cuts at:      0     552     993 
   Size:        552     441
 
SfcI C'TryA_G

Cuts at:      0     376     993 
   Size:        376     617
 
SphI G_CATG'C

Cuts at:      0     945     993 
   Size:        945      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     839     871     993 
   Size:        839      32     122

  Fragments arranged by size: 

                 839     122      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     241     993 
   Size:        241     752
 
XmnI GAAnn'nnTTC

Cuts at:      0     209     956     993 
   Size:        209     747      37

  Fragments arranged by size: 

                 747     209      37
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI    BamHI    BanII     BbsI
    BglII Bme1580I     BpmI   Bpu10I    BsaBI    BsaWI    BsaXI    BseYI
 Bsp1286I    BstYI   Bsu36I    BtgZI     EarI  Eco57MI EcoO109I     FalI
  HindIII     MslI     NspI     PpiI    PpuMI     PsrI    SexAI     SfcI
     SphI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI