Restriction map for
pF1KB6497
(Linear) (Six-base) MAPSORT of: KIBB6497.seq Check: 1503 from: 1 to: 1053
KIBB6497.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 534 813 1053
Size: 534 279 240
Fragments arranged by size:
534 279 240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1053
Size: 523 530
AfeI AGC'GCT
Cuts at: 0 841 1053
Size: 841 212
AlwNI CAG_nnn'CTG
Cuts at: 0 964 1053
Size: 964 89
ApaI G_GGCC'C
Cuts at: 0 672 1053
Size: 672 381
AvaI C'yCGr_G
Cuts at: 0 508 622 1053
Size: 508 114 431
Fragments arranged by size:
508 431 114
AvrII C'CTAG_G
Cuts at: 0 672 1053
Size: 672 381
BanI G'GyrC_C
Cuts at: 0 185 1053
Size: 185 868
BanII G_rGCy'C
Cuts at: 0 160 425 672 1007 1053
Size: 160 265 247 335 46
Fragments arranged by size:
335 265 247 160 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 443 1053
Size: 409 34 610
Fragments arranged by size:
610 409 34
BglII A'GATC_T
Cuts at: 0 737 1053
Size: 737 316
Bme1580I G_kGCm'C
Cuts at: 0 672 1053
Size: 672 381
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 97 405 1053
Size: 97 308 648
Fragments arranged by size:
648 308 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1053
Size: 304 749
Bpu10I CC'TnA_GC
Cuts at: 0 139 634 1053
Size: 139 495 419
Fragments arranged by size:
495 419 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1053
Size: 781 272
BsaI GGTCTCn'nnnn_
Cuts at: 0 187 1053
Size: 187 866
BsaAI yAC'GTr
Cuts at: 0 369 1053
Size: 369 684
BsaBI GATnn'nnATC
Cuts at: 0 913 1015 1053
Size: 913 102 38
Fragments arranged by size:
913 102 38
BsaWI w'CCGG_w
Cuts at: 0 1007 1053
Size: 1007 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 86 508 1009 1053
Size: 52 34 422 501 44
Fragments arranged by size:
501 422 52 44 34
BseYI C'CCAG_C
Cuts at: 0 147 1053
Size: 147 906
BsiEI CG_ry'CG
Cuts at: 0 296 1053
Size: 296 757
BsiHKAI G_wGCw'C
Cuts at: 0 263 683 1007 1053
Size: 263 420 324 46
Fragments arranged by size:
420 324 263 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 759 1053
Size: 759 294
Bsp1286I G_dGCh'C
Cuts at: 0 160 263 425 672 683 1007 1053
Size: 160 103 162 247 11 324 46
Fragments arranged by size:
324 247 162 160 103 46 11
BspEI T'CCGG_A
Cuts at: 0 1007 1053
Size: 1007 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 243 534 548 813 1053
Size: 243 291 14 265 240
Fragments arranged by size:
291 265 243 240 14
BsrBI CCG'CTC
Cuts at: 0 103 1053
Size: 103 950
BsrFI r'CCGG_y
Cuts at: 0 196 1053
Size: 196 857
BstAPI GCAn_nnn'nTGC
Cuts at: 0 807 1053
Size: 807 246
BstBI TT'CG_AA
Cuts at: 0 131 1053
Size: 131 922
BstYI r'GATC_y
Cuts at: 0 218 737 1010 1053
Size: 218 519 273 43
Fragments arranged by size:
519 273 218 43
Bsu36I CC'TnA_GG
Cuts at: 0 853 1053
Size: 853 200
BtgI C'CryG_G
Cuts at: 0 656 698 1053
Size: 656 42 355
Fragments arranged by size:
656 355 42
EaeI y'GGCC_r
Cuts at: 0 201 458 695 1053
Size: 201 257 237 358
Fragments arranged by size:
358 257 237 201
EarI CTCTTCn'nnn_
Cuts at: 0 995 1053
Size: 995 58
EcoICRI GAG'CTC
Cuts at: 0 1005 1053
Size: 1005 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 523 1053
Size: 304 219 530
Fragments arranged by size:
530 304 219
EcoNI CCTnn'n_nnAGG
Cuts at: 0 820 1053
Size: 820 233
EcoO109I rG'GnC_Cy
Cuts at: 0 602 669 961 1053
Size: 602 67 292 92
Fragments arranged by size:
602 292 92 67
HaeII r_GCGC'y
Cuts at: 0 843 1053
Size: 843 210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 1053
Size: 387 666
MspA1I CmG'CkG
Cuts at: 0 905 1053
Size: 905 148
PasI CC'CwG_GG
Cuts at: 0 964 1053
Size: 964 89
PpuMI rG'GwC_Cy
Cuts at: 0 602 1053
Size: 602 451
PspOMI G'GGCC_C
Cuts at: 0 668 1053
Size: 668 385
PstI C_TGCA'G
Cuts at: 0 290 545 824 1053
Size: 290 255 279 229
Fragments arranged by size:
290 279 255 229
PvuI CG_AT'CG
Cuts at: 0 296 1053
Size: 296 757
PvuII CAG'CTG
Cuts at: 0 905 1053
Size: 905 148
SacI G_AGCT'C
Cuts at: 0 1007 1053
Size: 1007 46
SanDI GG'GwC_CC
Cuts at: 0 602 1053
Size: 602 451
SapI GCTCTTCn'nnn_
Cuts at: 0 995 1053
Size: 995 58
SbfI CC_TGCA'GG
Cuts at: 0 290 545 1053
Size: 290 255 508
Fragments arranged by size:
508 290 255
SexAI A'CCwGG_T
Cuts at: 0 353 1053
Size: 353 700
SfcI C'TryA_G
Cuts at: 0 84 286 397 541 820 1053
Size: 84 202 111 144 279 233
Fragments arranged by size:
279 233 202 144 111 84
SmlI C'TyrA_G
Cuts at: 0 796 1053
Size: 796 257
StyI C'CwwG_G
Cuts at: 0 672 1034 1053
Size: 672 362 19
Fragments arranged by size:
672 362 19
TatI w'GTAC_w
Cuts at: 0 953 1053
Size: 953 100
XmnI GAAnn'nnTTC
Cuts at: 0 52 1053
Size: 52 1001
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI AvrII BanI
BanII BcgI BglII Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstBI
BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII MmeI MspA1I PasI PpuMI PspOMI PstI
PvuI PvuII SacI SanDI SapI SbfI SexAI SfcI
SmlI StyI TatI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BsaHI BsaXI BsgI
BsiWI BsmI BspHI BsrDI BsrGI BssHII BssSI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI