(Linear) (Six-base) MAPSORT of: KIBB6499.seq  Check: 7817  from: 1  to: 309



KIBB6499.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0     263     309 
   Size:        263      46
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     292     309 
   Size:        196      96      17

  Fragments arranged by size: 

                 196      96      17
 
BanI G'GyrC_C

Cuts at:      0     107     309 
   Size:        107     202
 
BbeI G_GCGC'C

Cuts at:      0     111     309 
   Size:        111     198
 
BlpI GC'TnA_GC

Cuts at:      0      47     309 
   Size:         47     262
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      40     309 
   Size:         40     269
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     309 
   Size:        151     158
 
BsaHI Gr'CG_yC

Cuts at:      0     108     309 
   Size:        108     201
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     309 
   Size:        227      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     309 
   Size:        227      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     265     309 
   Size:        265      44
 
EarI CTCTTCn'nnn_

Cuts at:      0     268     309 
   Size:        268      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      35     309 
   Size:         35     274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     292     309 
   Size:        196      96      17

  Fragments arranged by size: 

                 196      96      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63     309 
   Size:         63     246
 
HaeII r_GCGC'y

Cuts at:      0      47     111     309 
   Size:         47      64     198

  Fragments arranged by size: 

                 198      64      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      62      94     309 
   Size:         62      32     215

  Fragments arranged by size: 

                 215      62      32
 
KasI G'GCGC_C

Cuts at:      0     107     309 
   Size:        107     202
 
NarI GG'CG_CC

Cuts at:      0     108     309 
   Size:        108     201
 
PpuMI rG'GwC_Cy

Cuts at:      0      63     309 
   Size:         63     246
 
SfoI GGC'GCC

Cuts at:      0     109     309 
   Size:        109     200
 

 Enzymes that do cut:

     AccI     AcuI     BanI     BbeI     BlpI     BmrI     BsaI    BsaHI
    BseRI  BsiHKAI Bsp1286I    BtgZI     EarI     EciI  Eco57MI EcoO109I
    HaeII    Hin4I     KasI     NarI    PpuMI     SfoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI