Restriction map for
pF1KB6505
(Linear) (Six-base) MAPSORT of: KIBB6505.seq Check: 5092 from: 1 to: 1074
KIBB6505.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 280 1074
Size: 280 794
Acc65I G'GTAC_C
Cuts at: 0 385 1074
Size: 385 689
AflIII A'CryG_T
Cuts at: 0 659 1074
Size: 659 415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 946 1074
Size: 946 128
AlwNI CAG_nnn'CTG
Cuts at: 0 255 274 1074
Size: 255 19 800
Fragments arranged by size:
800 255 19
ApaI G_GGCC'C
Cuts at: 0 116 209 1074
Size: 116 93 865
Fragments arranged by size:
865 116 93
AvaI C'yCGr_G
Cuts at: 0 226 711 1074
Size: 226 485 363
Fragments arranged by size:
485 363 226
BanI G'GyrC_C
Cuts at: 0 385 1074
Size: 385 689
BanII G_rGCy'C
Cuts at: 0 105 116 154 209 227 329 1074
Size: 105 11 38 55 18 102 745
Fragments arranged by size:
745 105 102 55 38 18 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 558 1074
Size: 558 516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 343 377 833 867 1074
Size: 343 34 456 34 207
Fragments arranged by size:
456 343 207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 469 1074
Size: 469 605
BglI GCCn_nnn'nGGC
Cuts at: 0 250 269 1074
Size: 250 19 805
Fragments arranged by size:
805 250 19
Bme1580I G_kGCm'C
Cuts at: 0 116 209 667 1074
Size: 116 93 458 407
Fragments arranged by size:
458 407 116 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 638 1027 1074
Size: 638 389 47
Fragments arranged by size:
638 389 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 241 343 619 628 753 1074
Size: 149 92 102 276 9 125 321
Fragments arranged by size:
321 276 149 125 102 92 9
Bpu10I CC'TnA_GC
Cuts at: 0 148 464 963 1074
Size: 148 316 499 111
Fragments arranged by size:
499 316 148 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1074
Size: 532 542
BsaAI yAC'GTr
Cuts at: 0 675 1074
Size: 675 399
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 385 1057 1074
Size: 244 141 672 17
Fragments arranged by size:
672 244 141 17
BseYI C'CCAG_C
Cuts at: 0 116 389 666 1074
Size: 116 273 277 408
Fragments arranged by size:
408 277 273 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 916 1074
Size: 241 675 158
Fragments arranged by size:
675 241 158
BsiHKAI G_wGCw'C
Cuts at: 0 105 485 1074
Size: 105 380 589
Fragments arranged by size:
589 380 105
Bsp1286I G_dGCh'C
Cuts at: 0 105 116 154 209 227 329 485 667
Size: 105 11 38 55 18 102 156 182
Cuts at: 667 1074
Size: 407
Fragments arranged by size:
407 182 156 105 102 55 38 18
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 1074
Size: 292 782
BsrFI r'CCGG_y
Cuts at: 0 766 1074
Size: 766 308
BstXI CCAn_nnnn'nTGG
Cuts at: 0 15 507 1074
Size: 15 492 567
Fragments arranged by size:
567 492 15
BstYI r'GATC_y
Cuts at: 0 815 1074
Size: 815 259
BstZ17I GTA'TAC
Cuts at: 0 281 1074
Size: 281 793
Bsu36I CC'TnA_GG
Cuts at: 0 470 1074
Size: 470 604
BtgI C'CryG_G
Cuts at: 0 420 822 1074
Size: 420 402 252
Fragments arranged by size:
420 402 252
BtsI GCAGTG_nn'
Cuts at: 0 188 258 1074
Size: 188 70 816
Fragments arranged by size:
816 188 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 164 199 1074
Size: 164 35 875
Fragments arranged by size:
875 164 35
EaeI y'GGCC_r
Cuts at: 0 185 314 497 764 768 1074
Size: 185 129 183 267 4 306
Fragments arranged by size:
306 267 185 183 129 4
EarI CTCTTCn'nnn_
Cuts at: 0 490 757 811 1034 1074
Size: 490 267 54 223 40
Fragments arranged by size:
490 267 223 54 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 478 808 1074
Size: 478 330 266
Fragments arranged by size:
478 330 266
EcoICRI GAG'CTC
Cuts at: 0 103 1074
Size: 103 971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 241 343 619 628 753 1074
Size: 149 92 102 276 9 125 321
Fragments arranged by size:
321 276 149 125 102 92 9
EcoO109I rG'GnC_Cy
Cuts at: 0 113 206 252 375 679 827 951 1074
Size: 113 93 46 123 304 148 124 123
Fragments arranged by size:
304 148 124 123 123 113 93 46
FseI GG_CCGG'CC
Cuts at: 0 770 1074
Size: 770 304
HaeII r_GCGC'y
Cuts at: 0 751 1074
Size: 751 323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 968 1000 1074
Size: 968 32 74
Fragments arranged by size:
968 74 32
KpnI G_GTAC'C
Cuts at: 0 389 1074
Size: 389 685
MluI A'CGCG_T
Cuts at: 0 659 1074
Size: 659 415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 41 1074
Size: 35 6 1033
Fragments arranged by size:
1033 35 6
MscI TGG'CCA
Cuts at: 0 187 499 1074
Size: 187 312 575
Fragments arranged by size:
575 312 187
MslI CAynn'nnrTG
Cuts at: 0 353 1074
Size: 353 721
MspA1I CmG'CkG
Cuts at: 0 824 866 901 1074
Size: 824 42 35 173
Fragments arranged by size:
824 173 42 35
NaeI GCC'GGC
Cuts at: 0 768 1074
Size: 768 306
NcoI C'CATG_G
Cuts at: 0 420 1074
Size: 420 654
NgoMIV G'CCGG_C
Cuts at: 0 766 1074
Size: 766 308
NspI r_CATG'y
Cuts at: 0 358 432 1074
Size: 358 74 642
Fragments arranged by size:
642 358 74
PfoI T'CCnGG_A
Cuts at: 0 40 218 302 782 1074
Size: 40 178 84 480 292
Fragments arranged by size:
480 292 178 84 40
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1046 1074
Size: 1046 28
PpuMI rG'GwC_Cy
Cuts at: 0 375 679 951 1074
Size: 375 304 272 123
Fragments arranged by size:
375 304 272 123
PspOMI G'GGCC_C
Cuts at: 0 112 205 1074
Size: 112 93 869
Fragments arranged by size:
869 112 93
PstI C_TGCA'G
Cuts at: 0 846 1074
Size: 846 228
PvuII CAG'CTG
Cuts at: 0 901 1074
Size: 901 173
SacI G_AGCT'C
Cuts at: 0 105 1074
Size: 105 969
SacII CC_GC'GG
Cuts at: 0 825 1074
Size: 825 249
SapI GCTCTTCn'nnn_
Cuts at: 0 490 757 811 1074
Size: 490 267 54 263
Fragments arranged by size:
490 267 263 54
SbfI CC_TGCA'GG
Cuts at: 0 846 1074
Size: 846 228
SfcI C'TryA_G
Cuts at: 0 266 842 1074
Size: 266 576 232
Fragments arranged by size:
576 266 232
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 250 1074
Size: 250 824
SmlI C'TyrA_G
Cuts at: 0 547 1074
Size: 547 527
StuI AGG'CCT
Cuts at: 0 848 1074
Size: 848 226
StyI C'CwwG_G
Cuts at: 0 420 1074
Size: 420 654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1074
Size: 375 699
XmnI GAAnn'nnTTC
Cuts at: 0 1061 1074
Size: 1061 13
Enzymes that do cut:
AccI Acc65I AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbsI BcgI BciVI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtsI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FseI
HaeII Hin4I KpnI MluI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SapI SbfI SfcI SfiI
SmlI StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsaI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI ClaI
DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KasI MfeI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI ZraI