Restriction map for
pF1KB6509
(Linear) (Six-base) MAPSORT of: KIBB6509.seq Check: 2614 from: 1 to: 1077
KIBB6509.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 890 1040 1077
Size: 890 150 37
Fragments arranged by size:
890 150 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 439 442 577 1014 1077
Size: 331 108 3 135 437 63
Fragments arranged by size:
437 331 135 108 63 3
AleI CACnn'nnGTG
Cuts at: 0 490 1077
Size: 490 587
AlwNI CAG_nnn'CTG
Cuts at: 0 462 1077
Size: 462 615
AvrII C'CTAG_G
Cuts at: 0 147 1077
Size: 147 930
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 833 866 1077
Size: 833 33 211
Fragments arranged by size:
833 211 33
BanI G'GyrC_C
Cuts at: 0 544 1077
Size: 544 533
BanII G_rGCy'C
Cuts at: 0 143 565 1077
Size: 143 422 512
Fragments arranged by size:
512 422 143
BbsI GAAGACnn'nnnn_
Cuts at: 0 646 1077
Size: 646 431
Bme1580I G_kGCm'C
Cuts at: 0 163 471 547 1077
Size: 163 308 76 530
Fragments arranged by size:
530 308 163 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 148 550 1017 1077
Size: 148 402 467 60
Fragments arranged by size:
467 402 148 60
BmtI G_CTAG'C
Cuts at: 0 719 1077
Size: 719 358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1077
Size: 386 691
Bpu10I CC'TnA_GC
Cuts at: 0 564 1077
Size: 564 513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1077
Size: 632 445
BsaAI yAC'GTr
Cuts at: 0 1044 1077
Size: 1044 33
BsiHKAI G_wGCw'C
Cuts at: 0 565 1077
Size: 565 512
BsmI GAATG_Cn'
Cuts at: 0 641 1077
Size: 641 436
Bsp1286I G_dGCh'C
Cuts at: 0 143 163 471 547 565 1077
Size: 143 20 308 76 18 512
Fragments arranged by size:
512 308 143 76 20 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 1077
Size: 269 808
BsrDI GCAATG_nn'
Cuts at: 0 617 1077
Size: 617 460
BstYI r'GATC_y
Cuts at: 0 231 1077
Size: 231 846
EaeI y'GGCC_r
Cuts at: 0 794 1077
Size: 794 283
EarI CTCTTCn'nnn_
Cuts at: 0 458 553 1077
Size: 458 95 524
Fragments arranged by size:
524 458 95
EcoICRI GAG'CTC
Cuts at: 0 563 1077
Size: 563 514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 386 439 442 577 1014 1077
Size: 331 55 53 3 135 437 63
Fragments arranged by size:
437 331 135 63 55 53 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 1077
Size: 772 32 273
Fragments arranged by size:
772 273 32
HincII GTy'rAC
Cuts at: 0 339 780 1077
Size: 339 441 297
Fragments arranged by size:
441 339 297
HindIII A'AGCT_T
Cuts at: 0 838 1077
Size: 838 239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 900 1077
Size: 371 529 177
Fragments arranged by size:
529 371 177
MscI TGG'CCA
Cuts at: 0 796 1077
Size: 796 281
MslI CAynn'nnrTG
Cuts at: 0 476 490 683 1077
Size: 476 14 193 394
Fragments arranged by size:
476 394 193 14
MspA1I CmG'CkG
Cuts at: 0 170 420 555 1077
Size: 170 250 135 522
Fragments arranged by size:
522 250 170 135
NheI G'CTAG_C
Cuts at: 0 715 1077
Size: 715 362
PfoI T'CCnGG_A
Cuts at: 0 363 1077
Size: 363 714
PvuII CAG'CTG
Cuts at: 0 420 1077
Size: 420 657
SacI G_AGCT'C
Cuts at: 0 565 1077
Size: 565 512
SapI GCTCTTCn'nnn_
Cuts at: 0 553 1077
Size: 553 524
ScaI AGT'ACT
Cuts at: 0 1006 1077
Size: 1006 71
SexAI A'CCwGG_T
Cuts at: 0 852 1077
Size: 852 225
SfcI C'TryA_G
Cuts at: 0 914 1077
Size: 914 163
SmlI C'TyrA_G
Cuts at: 0 647 1077
Size: 647 430
StyI C'CwwG_G
Cuts at: 0 147 1077
Size: 147 930
TatI w'GTAC_w
Cuts at: 0 875 1004 1077
Size: 875 129 73
Fragments arranged by size:
875 129 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 627 659 1077
Size: 627 32 418
Fragments arranged by size:
627 418 32
Enzymes that do cut:
AccI AcuI AleI AlwNI AvrII BaeI BanI BanII
BbsI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaAI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BstYI EaeI EarI
EcoICRI Eco57MI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NheI PfoI PvuII SacI SapI ScaI SexAI
SfcI SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BplI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI