Restriction map for
pF1KB6510
(Linear) (Six-base) MAPSORT of: KIBB6510.seq Check: 4517 from: 1 to: 1152
KIBB6510.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 559 686 1152
Size: 58 501 127 466
Fragments arranged by size:
501 466 127 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 890 1152
Size: 296 594 262
Fragments arranged by size:
594 296 262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 1152
Size: 674 32 446
Fragments arranged by size:
674 446 32
AlwNI CAG_nnn'CTG
Cuts at: 0 550 1152
Size: 550 602
ApaLI G'TGCA_C
Cuts at: 0 221 1152
Size: 221 931
ApoI r'AATT_y
Cuts at: 0 109 138 1152
Size: 109 29 1014
Fragments arranged by size:
1014 109 29
BanI G'GyrC_C
Cuts at: 0 967 1152
Size: 967 185
BanII G_rGCy'C
Cuts at: 0 107 980 1152
Size: 107 873 172
Fragments arranged by size:
873 172 107
BglI GCCn_nnn'nGGC
Cuts at: 0 367 1152
Size: 367 785
BglII A'GATC_T
Cuts at: 0 171 1017 1152
Size: 171 846 135
Fragments arranged by size:
846 171 135
BlpI GC'TnA_GC
Cuts at: 0 263 1152
Size: 263 889
Bme1580I G_kGCm'C
Cuts at: 0 225 770 1152
Size: 225 545 382
Fragments arranged by size:
545 382 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 1152
Size: 324 828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 751 1152
Size: 289 462 401
Fragments arranged by size:
462 401 289
BsaBI GATnn'nnATC
Cuts at: 0 683 1152
Size: 683 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 802 896 926 1152
Size: 772 30 94 30 226
Fragments arranged by size:
772 226 94 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 814 817 1152
Size: 190 624 3 335
Fragments arranged by size:
624 335 190 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 563 886 1152
Size: 181 382 323 266
Fragments arranged by size:
382 323 266 181
BsiHKAI G_wGCw'C
Cuts at: 0 225 980 1152
Size: 225 755 172
Fragments arranged by size:
755 225 172
Bsp1286I G_dGCh'C
Cuts at: 0 107 225 770 980 1152
Size: 107 118 545 210 172
Fragments arranged by size:
545 210 172 118 107
BspHI T'CATG_A
Cuts at: 0 795 1152
Size: 795 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 934 1152
Size: 934 218
BstYI r'GATC_y
Cuts at: 0 171 644 1017 1152
Size: 171 473 373 135
Fragments arranged by size:
473 373 171 135
BtgI C'CryG_G
Cuts at: 0 64 1152
Size: 64 1088
ClaI AT'CG_AT
Cuts at: 0 75 1152
Size: 75 1077
EaeI y'GGCC_r
Cuts at: 0 178 336 983 1106 1152
Size: 178 158 647 123 46
Fragments arranged by size:
647 178 158 123 46
EarI CTCTTCn'nnn_
Cuts at: 0 662 1152
Size: 662 490
EcoICRI GAG'CTC
Cuts at: 0 978 1152
Size: 978 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 289 559 686 751 1152
Size: 58 231 270 127 65 401
Fragments arranged by size:
401 270 231 127 65 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 796 1152
Size: 796 356
EcoRV GAT'ATC
Cuts at: 0 414 1152
Size: 414 738
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 886 1152
Size: 886 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 466 498 772 804 1152
Size: 398 32 36 32 274 32 348
Fragments arranged by size:
398 348 274 36 32 32 32
MscI TGG'CCA
Cuts at: 0 180 338 985 1108 1152
Size: 180 158 647 123 44
Fragments arranged by size:
647 180 158 123 44
MslI CAynn'nnrTG
Cuts at: 0 257 656 1152
Size: 257 399 496
Fragments arranged by size:
496 399 257
MspA1I CmG'CkG
Cuts at: 0 66 276 1152
Size: 66 210 876
Fragments arranged by size:
876 210 66
NruI TCG'CGA
Cuts at: 0 55 1152
Size: 55 1097
PfoI T'CCnGG_A
Cuts at: 0 26 1152
Size: 26 1126
PstI C_TGCA'G
Cuts at: 0 326 348 376 515 1152
Size: 326 22 28 139 637
Fragments arranged by size:
637 326 139 28 22
PvuII CAG'CTG
Cuts at: 0 276 1152
Size: 276 876
SacI G_AGCT'C
Cuts at: 0 980 1152
Size: 980 172
SacII CC_GC'GG
Cuts at: 0 67 1152
Size: 67 1085
ScaI AGT'ACT
Cuts at: 0 463 1152
Size: 463 689
SfcI C'TryA_G
Cuts at: 0 322 344 372 511 637 1152
Size: 322 22 28 139 126 515
Fragments arranged by size:
515 322 139 126 28 22
StyI C'CwwG_G
Cuts at: 0 284 1152
Size: 284 868
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 439 1152
Size: 439 713
TatI w'GTAC_w
Cuts at: 0 461 1124 1152
Size: 461 663 28
Fragments arranged by size:
663 461 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 978 1152
Size: 978 174
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaLI ApoI BanI BanII
BglI BglII BlpI Bme1580I BmrI BpmI BsaBI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BstYI BtgI
ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoRV FalI
Hin4I MscI MslI MspA1I NruI PfoI PstI PvuII
SacI SacII ScaI SfcI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EagI EciI EcoO109I EcoRI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI