(Linear) (Six-base) MAPSORT of: KIBB6519.seq  Check: 1656  from: 1  to: 1086



KIBB6519.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AccI GT'mk_AC

Cuts at:      0     883    1086 
   Size:        883     203
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1086 
   Size:        329     757
 
AvaI C'yCGr_G

Cuts at:      0     398    1086 
   Size:        398     688
 
BanII G_rGCy'C

Cuts at:      0     171     242     399    1086 
   Size:        171      71     157     687

  Fragments arranged by size: 

                 687     171     157      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1051    1086 
   Size:       1051      35
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     885    1086 
   Size:        885     201
 
BclI T'GATC_A

Cuts at:      0    1047    1086 
   Size:       1047      39
 
BglI GCCn_nnn'nGGC

Cuts at:      0      23     124    1086 
   Size:         23     101     962

  Fragments arranged by size: 

                 962     101      23
 
BglII A'GATC_T

Cuts at:      0     372    1086 
   Size:        372     714
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     162    1062    1086 
   Size:        162     900      24

  Fragments arranged by size: 

                 900     162      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     114    1086 
   Size:        114     972
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     737    1086 
   Size:         40     697     349

  Fragments arranged by size: 

                 697     349      40
 
BsaBI GATnn'nnATC

Cuts at:      0     788    1086 
   Size:        788     298
 
BsaWI w'CCGG_w

Cuts at:      0     192    1086 
   Size:        192     894
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     214    1086 
   Size:        214     872
 
BsiEI CG_ry'CG

Cuts at:      0     104    1086 
   Size:        104     982
 
BsiHKAI G_wGCw'C

Cuts at:      0     688    1086 
   Size:        688     398
 
Bsp1286I G_dGCh'C

Cuts at:      0     171     242     399     688    1086 
   Size:        171      71     157     289     398

  Fragments arranged by size: 

                 398     289     171     157      71
 
BspHI T'CATG_A

Cuts at:      0     519    1086 
   Size:        519     567
 
BsrDI GCAATG_nn'

Cuts at:      0     873    1086 
   Size:        873     213
 
BsrGI T'GTAC_A

Cuts at:      0     425    1086 
   Size:        425     661
 
BstEII G'GTnAC_C

Cuts at:      0     807    1086 
   Size:        807     279
 
BstYI r'GATC_y

Cuts at:      0     372    1086 
   Size:        372     714
 
BstZ17I GTA'TAC

Cuts at:      0     884    1086 
   Size:        884     202
 
Bsu36I CC'TnA_GG

Cuts at:      0      59    1086 
   Size:         59    1027
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     128    1086 
   Size:        128     958
 
BtsI GCAGTG_nn'

Cuts at:      0     128    1086 
   Size:        128     958
 
DraI TTT'AAA

Cuts at:      0     734    1086 
   Size:        734     352
 
EaeI y'GGCC_r

Cuts at:      0     140    1086 
   Size:        140     946
 
EarI CTCTTCn'nnn_

Cuts at:      0      41     481     968    1086 
   Size:         41     440     487     118

  Fragments arranged by size: 

                 487     440     118      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55    1086 
   Size:         55    1031
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     329     737    1086 
   Size:         40     289     408     349

  Fragments arranged by size: 

                 408     349     289      40
 
HaeII r_GCGC'y

Cuts at:      0     209    1086 
   Size:        209     877
 
HincII GTy'rAC

Cuts at:      0     255    1086 
   Size:        255     831
 
MslI CAynn'nnrTG

Cuts at:      0     638    1086 
   Size:        638     448
 
NspI r_CATG'y

Cuts at:      0     339     637     700    1086 
   Size:        339     298      63     386

  Fragments arranged by size: 

                 386     339     298      63
 
PspXI vC'TCGA_Gb

Cuts at:      0     398    1086 
   Size:        398     688
 
SfcI C'TryA_G

Cuts at:      0     448     764    1086 
   Size:        448     316     322

  Fragments arranged by size: 

                 448     322     316
 
SmlI C'TyrA_G

Cuts at:      0     398    1086 
   Size:        398     688
 
SphI G_CATG'C

Cuts at:      0     700    1086 
   Size:        700     386
 
SspI AAT'ATT

Cuts at:      0     360     953    1082    1086 
   Size:        360     593     129       4

  Fragments arranged by size: 

                 593     360     129       4
 
StyI C'CwwG_G

Cuts at:      0     209    1086 
   Size:        209     877
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      90    1086 
   Size:         90     996
 
TatI w'GTAC_w

Cuts at:      0     425    1086 
   Size:        425     661
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     837    1086 
   Size:        837     249
 
XhoI C'TCGA_G

Cuts at:      0     398    1086 
   Size:        398     688
 
XmnI GAAnn'nnTTC

Cuts at:      0     458    1086 
   Size:        458     628
 

 Enzymes that do cut:

     AccI     AcuI     AvaI    BanII     BbsI    BciVI     BclI     BglI
    BglII     BmrI     BplI     BpmI    BsaBI    BsaWI    BseRI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrGI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     DraI     EaeI     EarI     EciI  Eco57MI
    HaeII   HincII     MslI     NspI    PspXI     SfcI     SmlI     SphI
     SspI     StyI    TaqII     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BfrBI     BlpI Bme1580I    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseYI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI     BtgI     ClaI    CspCI   DraIII     DrdI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI
     TstI  Tth111I     XbaI     XmaI     ZraI