(Linear) (Six-base) MAPSORT of: KIBB6521.seq  Check: 3270  from: 1  to: 1089



KIBB6521.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AfeI AGC'GCT

Cuts at:      0     741    1089 
   Size:        741     348
 
AflII C'TTAA_G

Cuts at:      0     711    1089 
   Size:        711     378
 
ApoI r'AATT_y

Cuts at:      0     372     586     614    1089 
   Size:        372     214      28     475

  Fragments arranged by size: 

                 475     372     214      28
 
BbvCI CC'TCA_GC

Cuts at:      0     468    1089 
   Size:        468     621
 
BfrBI ATG'CAT

Cuts at:      0     328    1089 
   Size:        328     761
 
BglII A'GATC_T

Cuts at:      0     207    1089 
   Size:        207     882
 
Bpu10I CC'TnA_GC

Cuts at:      0     468     476    1089 
   Size:        468       8     613

  Fragments arranged by size: 

                 613     468       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337    1089 
   Size:        337     752
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     493     523    1089 
   Size:        493      30     566

  Fragments arranged by size: 

                 566     493      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      74    1089 
   Size:         74    1015
 
BseYI C'CCAG_C

Cuts at:      0     796    1089 
   Size:        796     293
 
BsrDI GCAATG_nn'

Cuts at:      0     638     791    1089 
   Size:        638     153     298

  Fragments arranged by size: 

                 638     298     153
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1021    1089 
   Size:       1021      68
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264    1089 
   Size:        264     825
 
BstYI r'GATC_y

Cuts at:      0     207    1089 
   Size:        207     882
 
BtgI C'CryG_G

Cuts at:      0     497    1089 
   Size:        497     592
 
BtsI GCAGTG_nn'

Cuts at:      0     185    1089 
   Size:        185     904
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     125     160    1089 
   Size:        125      35     929

  Fragments arranged by size: 

                 929     125      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     413    1089 
   Size:        413     676
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      28    1089 
   Size:         28    1061
 
HaeII r_GCGC'y

Cuts at:      0     743    1089 
   Size:        743     346
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      39      71     493     525    1089 
   Size:         39      32     422      32     564

  Fragments arranged by size: 

                 564     422      39      32      32
 
HincII GTy'rAC

Cuts at:      0    1002    1089 
   Size:       1002      87
 
MspA1I CmG'CkG

Cuts at:      0      45     472     811    1089 
   Size:         45     427     339     278

  Fragments arranged by size: 

                 427     339     278      45
 
NcoI C'CATG_G

Cuts at:      0     497    1089 
   Size:        497     592
 
NsiI A_TGCA'T

Cuts at:      0     330    1089 
   Size:        330     759
 
PasI CC'CwG_GG

Cuts at:      0     116    1089 
   Size:        116     973
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     936    1089 
   Size:        936     153
 
PfoI T'CCnGG_A

Cuts at:      0     161    1089 
   Size:        161     928
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     329     361    1089 
   Size:        329      32     728

  Fragments arranged by size: 

                 728     329      32
 
PvuII CAG'CTG

Cuts at:      0     472     811    1089 
   Size:        472     339     278

  Fragments arranged by size: 

                 472     339     278
 
ScaI AGT'ACT

Cuts at:      0      33    1089 
   Size:         33    1056
 
SmlI C'TyrA_G

Cuts at:      0     316     711    1089 
   Size:        316     395     378

  Fragments arranged by size: 

                 395     378     316
 
StyI C'CwwG_G

Cuts at:      0      50     497    1089 
   Size:         50     447     592

  Fragments arranged by size: 

                 592     447      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     108    1089 
   Size:        108     981
 
TatI w'GTAC_w

Cuts at:      0      31    1089 
   Size:         31    1058
 
XbaI T'CTAG_A

Cuts at:      0     574    1089 
   Size:        574     515
 

 Enzymes that do cut:

     AfeI    AflII     ApoI    BbvCI    BfrBI    BglII   Bpu10I    BpuEI
    BsaXI    BseRI    BseYI    BsrDI   BstAPI    BstXI    BstYI     BtgI
     BtsI    CspCI     EciI     FalI    HaeII    Hin4I   HincII   MspA1I
     NcoI     NsiI     PasI    PflMI     PfoI     PsrI    PvuII     ScaI
     SmlI     StyI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI