Restriction map for
pF1KB6522
(Linear) (Six-base) MAPSORT of: KIBB6522.seq Check: 8184 from: 1 to: 1161
KIBB6522.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 755 925 1161
Size: 755 170 236
Fragments arranged by size:
755 236 170
ApoI r'AATT_y
Cuts at: 0 184 199 367 422 600 626 1161
Size: 184 15 168 55 178 26 535
Fragments arranged by size:
535 184 178 168 55 26 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 419 1161
Size: 419 742
BbvCI CC'TCA_GC
Cuts at: 0 1058 1161
Size: 1058 103
BclI T'GATC_A
Cuts at: 0 387 1161
Size: 387 774
BlpI GC'TnA_GC
Cuts at: 0 484 1161
Size: 484 677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1161
Size: 177 984
Bpu10I CC'TnA_GC
Cuts at: 0 1058 1161
Size: 1058 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 857 1161
Size: 857 304
BsaAI yAC'GTr
Cuts at: 0 152 822 1097 1161
Size: 152 670 275 64
Fragments arranged by size:
670 275 152 64
BsaBI GATnn'nnATC
Cuts at: 0 926 1161
Size: 926 235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 1161
Size: 25 1136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 545 565 955 1075 1161
Size: 545 20 390 120 86
Fragments arranged by size:
545 390 120 86 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1161
Size: 419 742
BsmBI CGTCTCn'nnnn_
Cuts at: 0 207 1161
Size: 207 954
BsrDI GCAATG_nn'
Cuts at: 0 283 1161
Size: 283 878
BsrGI T'GTAC_A
Cuts at: 0 810 1161
Size: 810 351
BssSI C'ACGA_G
Cuts at: 0 176 1161
Size: 176 985
BstYI r'GATC_y
Cuts at: 0 90 708 1161
Size: 90 618 453
Fragments arranged by size:
618 453 90
BtsI GCAGTG_nn'
Cuts at: 0 431 1161
Size: 431 730
DraI TTT'AAA
Cuts at: 0 86 1161
Size: 86 1075
EarI CTCTTCn'nnn_
Cuts at: 0 100 459 698 855 1161
Size: 100 359 239 157 306
Fragments arranged by size:
359 306 239 157 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1161
Size: 177 984
EcoO109I rG'GnC_Cy
Cuts at: 0 472 795 1161
Size: 472 323 366
Fragments arranged by size:
472 366 323
EcoRV GAT'ATC
Cuts at: 0 220 1032 1161
Size: 220 812 129
Fragments arranged by size:
812 220 129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 111 247 1161
Size: 111 136 914
Fragments arranged by size:
914 136 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 204 236 601 633 1161
Size: 27 177 32 365 32 528
Fragments arranged by size:
528 365 177 32 32 27
HindIII A'AGCT_T
Cuts at: 0 440 1161
Size: 440 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1161
Size: 418 743
MslI CAynn'nnrTG
Cuts at: 0 562 1076 1161
Size: 562 514 85
Fragments arranged by size:
562 514 85
MspA1I CmG'CkG
Cuts at: 0 740 1161
Size: 740 421
PpuMI rG'GwC_Cy
Cuts at: 0 472 1161
Size: 472 689
PsiI TTA'TAA
Cuts at: 0 632 1161
Size: 632 529
PvuII CAG'CTG
Cuts at: 0 740 1161
Size: 740 421
SnaBI TAC'GTA
Cuts at: 0 152 1161
Size: 152 1009
SspI AAT'ATT
Cuts at: 0 649 1161
Size: 649 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 835 1161
Size: 835 326
TatI w'GTAC_w
Cuts at: 0 810 1161
Size: 810 351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1131 1161
Size: 1131 30
XmnI GAAnn'nnTTC
Cuts at: 0 13 1161
Size: 13 1148
Enzymes that do cut:
AlwNI ApoI BbsI BbvCI BclI BlpI BpmI Bpu10I
BsaI BsaAI BsaBI BsaXI BseRI BsgI BsmBI BsrDI
BsrGI BssSI BstYI BtsI DraI EarI Eco57MI EcoO109I
EcoRV FalI Hin4I HindIII MmeI MslI MspA1I PpuMI
PsiI PvuII SnaBI SspI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BcgI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI StyI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI