Restriction map for
pF1KB6529
(Linear) (Six-base) MAPSORT of: KIBB6529.seq Check: 762 from: 1 to: 1095
KIBB6529.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 0 41 1095
Size: 41 1054
Acc65I G'GTAC_C
Cuts at: 0 560 1095
Size: 560 535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 472 511 1029 1095
Size: 247 225 39 518 66
Fragments arranged by size:
518 247 225 66 39
AflII C'TTAA_G
Cuts at: 0 412 1095
Size: 412 683
AflIII A'CryG_T
Cuts at: 0 74 1095
Size: 74 1021
AhdI GACnn_n'nnGTC
Cuts at: 0 135 618 1095
Size: 135 483 477
Fragments arranged by size:
483 477 135
AlwNI CAG_nnn'CTG
Cuts at: 0 387 705 747 783 1095
Size: 387 318 42 36 312
Fragments arranged by size:
387 318 312 42 36
ApaLI G'TGCA_C
Cuts at: 0 439 1095
Size: 439 656
AvaI C'yCGr_G
Cuts at: 0 571 1095
Size: 571 524
BanI G'GyrC_C
Cuts at: 0 560 720 1073 1095
Size: 560 160 353 22
Fragments arranged by size:
560 353 160 22
BanII G_rGCy'C
Cuts at: 0 260 570 1095
Size: 260 310 525
Fragments arranged by size:
525 310 260
BbeI G_GCGC'C
Cuts at: 0 1077 1095
Size: 1077 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 1095
Size: 261 834
BbvCI CC'TCA_GC
Cuts at: 0 583 1095
Size: 583 512
BglI GCCn_nnn'nGGC
Cuts at: 0 901 1082 1095
Size: 901 181 13
Fragments arranged by size:
901 181 13
BglII A'GATC_T
Cuts at: 0 188 1095
Size: 188 907
BlpI GC'TnA_GC
Cuts at: 0 166 1095
Size: 166 929
Bme1580I G_kGCm'C
Cuts at: 0 443 1095
Size: 443 652
BmgBI CAC'GTC
Cuts at: 0 90 876 1095
Size: 90 786 219
Fragments arranged by size:
786 219 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 886 1095
Size: 874 12 209
Fragments arranged by size:
874 209 12
Bpu10I CC'TnA_GC
Cuts at: 0 583 1095
Size: 583 512
BsaAI yAC'GTr
Cuts at: 0 75 546 1095
Size: 75 471 549
Fragments arranged by size:
549 471 75
BsaHI Gr'CG_yC
Cuts at: 0 38 527 1074 1095
Size: 38 489 547 21
Fragments arranged by size:
547 489 38 21
BsaWI w'CCGG_w
Cuts at: 0 11 1095
Size: 11 1084
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 273 385 1095
Size: 273 112 710
Fragments arranged by size:
710 273 112
BseYI C'CCAG_C
Cuts at: 0 276 430 959 1095
Size: 276 154 529 136
Fragments arranged by size:
529 276 154 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1095
Size: 760 335
BsiEI CG_ry'CG
Cuts at: 0 896 1095
Size: 896 199
BsiHKAI G_wGCw'C
Cuts at: 0 121 443 1095
Size: 121 322 652
Fragments arranged by size:
652 322 121
BsmBI CGTCTCn'nnnn_
Cuts at: 0 943 1095
Size: 943 152
Bsp1286I G_dGCh'C
Cuts at: 0 121 260 443 570 1095
Size: 121 139 183 127 525
Fragments arranged by size:
525 183 139 127 121
BspEI T'CCGG_A
Cuts at: 0 11 1095
Size: 11 1084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 857 1095
Size: 857 238
BsrDI GCAATG_nn'
Cuts at: 0 1052 1095
Size: 1052 43
BssHII G'CGCG_C
Cuts at: 0 201 1095
Size: 201 894
BssSI C'ACGA_G
Cuts at: 0 988 1095
Size: 988 107
BstEII G'GTnAC_C
Cuts at: 0 549 1095
Size: 549 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 430 1095
Size: 430 665
BstYI r'GATC_y
Cuts at: 0 188 336 377 701 1095
Size: 188 148 41 324 394
Fragments arranged by size:
394 324 188 148 41
Bsu36I CC'TnA_GG
Cuts at: 0 941 1095
Size: 941 154
BtgI C'CryG_G
Cuts at: 0 823 1095
Size: 823 272
DrdI GACnn_nn'nnGTC
Cuts at: 0 75 1095
Size: 75 1020
EaeI y'GGCC_r
Cuts at: 0 122 152 761 893 1095
Size: 122 30 609 132 202
Fragments arranged by size:
609 202 132 122 30
EagI C'GGCC_G
Cuts at: 0 893 1095
Size: 893 202
EarI CTCTTCn'nnn_
Cuts at: 0 941 1095
Size: 941 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 472 511 874 886 1029 1095
Size: 247 225 39 363 12 143 66
Fragments arranged by size:
363 247 225 143 66 39 12
EcoO109I rG'GnC_Cy
Cuts at: 0 101 447 903 936 1095
Size: 101 346 456 33 159
Fragments arranged by size:
456 346 159 101 33
FspI TGC'GCA
Cuts at: 0 292 1095
Size: 292 803
HaeII r_GCGC'y
Cuts at: 0 888 1077 1095
Size: 888 189 18
Fragments arranged by size:
888 189 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 604 636 1095
Size: 121 32 451 32 459
Fragments arranged by size:
459 451 121 32 32
HincII GTy'rAC
Cuts at: 0 42 1095
Size: 42 1053
KasI G'GCGC_C
Cuts at: 0 1073 1095
Size: 1073 22
KpnI G_GTAC'C
Cuts at: 0 564 1095
Size: 564 531
MscI TGG'CCA
Cuts at: 0 154 1095
Size: 154 941
MslI CAynn'nnrTG
Cuts at: 0 355 1095
Size: 355 740
MspA1I CmG'CkG
Cuts at: 0 98 557 587 693 816 1095
Size: 98 459 30 106 123 279
Fragments arranged by size:
459 279 123 106 98 30
NarI GG'CG_CC
Cuts at: 0 1074 1095
Size: 1074 21
NspI r_CATG'y
Cuts at: 0 237 523 1095
Size: 237 286 572
Fragments arranged by size:
572 286 237
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 609 1095
Size: 387 222 486
Fragments arranged by size:
486 387 222
PfoI T'CCnGG_A
Cuts at: 0 260 932 1095
Size: 260 672 163
Fragments arranged by size:
672 260 163
PpuMI rG'GwC_Cy
Cuts at: 0 447 936 1095
Size: 447 489 159
Fragments arranged by size:
489 447 159
PshAI GACnn'nnGTC
Cuts at: 0 88 1095
Size: 88 1007
PstI C_TGCA'G
Cuts at: 0 344 1095
Size: 344 751
PvuII CAG'CTG
Cuts at: 0 587 693 816 1095
Size: 587 106 123 279
Fragments arranged by size:
587 279 123 106
SanDI GG'GwC_CC
Cuts at: 0 936 1095
Size: 936 159
SexAI A'CCwGG_T
Cuts at: 0 311 386 1095
Size: 311 75 709
Fragments arranged by size:
709 311 75
SfcI C'TryA_G
Cuts at: 0 340 1095
Size: 340 755
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 901 1095
Size: 901 194
SfoI GGC'GCC
Cuts at: 0 1075 1095
Size: 1075 20
SmlI C'TyrA_G
Cuts at: 0 412 1095
Size: 412 683
StuI AGG'CCT
Cuts at: 0 410 1095
Size: 410 685
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1095
Size: 925 170
TatI w'GTAC_w
Cuts at: 0 416 1095
Size: 416 679
XmnI GAAnn'nnTTC
Cuts at: 0 805 1095
Size: 805 290
ZraI GAC'GTC
Cuts at: 0 39 1095
Size: 39 1056
Enzymes that do cut:
AatII Acc65I AcuI AflII AflIII AhdI AlwNI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BssHII BssSI BstEII BstXI BstYI Bsu36I
BtgI DrdI EaeI EagI EarI Eco57MI EcoO109I FspI
HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I
NarI NspI PflMI PfoI PpuMI PshAI PstI PvuII
SanDI SexAI SfcI SfiI SfoI SmlI StuI TaqII
TatI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AgeI AleI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaXI BsiWI BsmI BspHI BsrBI BsrFI
BsrGI BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI