Restriction map for
pF1KB6531
(Linear) (Six-base) MAPSORT of: KIBB6531.seq Check: 5034 from: 1 to: 1098
KIBB6531.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 388 498 975 1098
Size: 388 110 477 123
Fragments arranged by size:
477 388 123 110
ApaI G_GGCC'C
Cuts at: 0 232 781 874 1098
Size: 232 549 93 224
Fragments arranged by size:
549 232 224 93
ApaLI G'TGCA_C
Cuts at: 0 673 1098
Size: 673 425
ApoI r'AATT_y
Cuts at: 0 571 807 1098
Size: 571 236 291
Fragments arranged by size:
571 291 236
AvaI C'yCGr_G
Cuts at: 0 695 1098
Size: 695 403
BanI G'GyrC_C
Cuts at: 0 681 862 1098
Size: 681 181 236
Fragments arranged by size:
681 236 181
BanII G_rGCy'C
Cuts at: 0 232 427 751 781 856 874 902 1098
Size: 232 195 324 30 75 18 28 196
Fragments arranged by size:
324 232 196 195 75 30 28 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 136 757 1098
Size: 136 621 341
Fragments arranged by size:
621 341 136
BbvCI CC'TCA_GC
Cuts at: 0 263 458 1098
Size: 263 195 640
Fragments arranged by size:
640 263 195
BlpI GC'TnA_GC
Cuts at: 0 949 1098
Size: 949 149
Bme1580I G_kGCm'C
Cuts at: 0 232 677 781 867 874 1065 1098
Size: 232 445 104 86 7 191 33
Fragments arranged by size:
445 232 191 104 86 33 7
BmgBI CAC'GTC
Cuts at: 0 924 1098
Size: 924 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 391 1098
Size: 391 707
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 36 1098
Size: 36 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 334 1098
Size: 322 12 764
Fragments arranged by size:
764 322 12
Bpu10I CC'TnA_GC
Cuts at: 0 263 458 1098
Size: 263 195 640
Fragments arranged by size:
640 263 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1098
Size: 1060 38
BsaHI Gr'CG_yC
Cuts at: 0 133 1098
Size: 133 965
BsaWI w'CCGG_w
Cuts at: 0 366 1098
Size: 366 732
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 718 748 1098
Size: 718 30 350
Fragments arranged by size:
718 350 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 922 958 1098
Size: 25 897 36 140
Fragments arranged by size:
897 140 36 25
BseYI C'CCAG_C
Cuts at: 0 232 992 1098
Size: 232 760 106
Fragments arranged by size:
760 232 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 526 1098
Size: 201 325 572
Fragments arranged by size:
572 325 201
BsiHKAI G_wGCw'C
Cuts at: 0 489 677 902 1098
Size: 489 188 225 196
Fragments arranged by size:
489 225 196 188
BsmI GAATG_Cn'
Cuts at: 0 651 1098
Size: 651 447
Bsp1286I G_dGCh'C
Cuts at: 0 232 427 489 677 751 781 856 867
Size: 232 195 62 188 74 30 75 11
Cuts at: 867 874 902 1065 1098
Size: 7 28 163 33
Fragments arranged by size:
232 195 188 163 75 74 62 33
30 28 11 7
BsrDI GCAATG_nn'
Cuts at: 0 711 1098
Size: 711 387
BsrFI r'CCGG_y
Cuts at: 0 306 996 1098
Size: 306 690 102
Fragments arranged by size:
690 306 102
BstAPI GCAn_nnn'nTGC
Cuts at: 0 388 681 1098
Size: 388 293 417
Fragments arranged by size:
417 388 293
BstBI TT'CG_AA
Cuts at: 0 768 1098
Size: 768 330
BstEII G'GTnAC_C
Cuts at: 0 195 1098
Size: 195 903
BstXI CCAn_nnnn'nTGG
Cuts at: 0 550 633 744 1098
Size: 550 83 111 354
Fragments arranged by size:
550 354 111 83
BstYI r'GATC_y
Cuts at: 0 824 1098
Size: 824 274
Bsu36I CC'TnA_GG
Cuts at: 0 356 1098
Size: 356 742
BtgI C'CryG_G
Cuts at: 0 32 431 1041 1098
Size: 32 399 610 57
Fragments arranged by size:
610 399 57 32
BtsI GCAGTG_nn'
Cuts at: 0 349 1098
Size: 349 749
DraIII CAC_nnn'GTG
Cuts at: 0 681 1007 1098
Size: 681 326 91
Fragments arranged by size:
681 326 91
DrdI GACnn_nn'nnGTC
Cuts at: 0 546 1098
Size: 546 552
EaeI y'GGCC_r
Cuts at: 0 68 653 998 1098
Size: 68 585 345 100
Fragments arranged by size:
585 345 100 68
EarI CTCTTCn'nnn_
Cuts at: 0 2 351 1098
Size: 2 349 747
Fragments arranged by size:
747 349 2
EcoICRI GAG'CTC
Cuts at: 0 900 1098
Size: 900 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 334 1098
Size: 322 12 764
Fragments arranged by size:
764 322 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 103 1098
Size: 103 995
EcoO109I rG'GnC_Cy
Cuts at: 0 228 229 777 871 1098
Size: 228 1 548 94 227
Fragments arranged by size:
548 228 227 94 1
EcoRI G'AATT_C
Cuts at: 0 571 1098
Size: 571 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 809 841 1098
Size: 718 32 59 32 257
Fragments arranged by size:
718 257 59 32 32
MslI CAynn'nnrTG
Cuts at: 0 742 1098
Size: 742 356
MspA1I CmG'CkG
Cuts at: 0 215 262 388 1098
Size: 215 47 126 710
Fragments arranged by size:
710 215 126 47
NaeI GCC'GGC
Cuts at: 0 308 998 1098
Size: 308 690 100
Fragments arranged by size:
690 308 100
NcoI C'CATG_G
Cuts at: 0 431 1098
Size: 431 667
NgoMIV G'CCGG_C
Cuts at: 0 306 996 1098
Size: 306 690 102
Fragments arranged by size:
690 306 102
NspI r_CATG'y
Cuts at: 0 300 1098
Size: 300 798
PasI CC'CwG_GG
Cuts at: 0 849 858 867 1098
Size: 849 9 9 231
Fragments arranged by size:
849 231 9 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 975 1098
Size: 975 123
PspOMI G'GGCC_C
Cuts at: 0 228 777 870 1098
Size: 228 549 93 228
Fragments arranged by size:
549 228 228 93
PstI C_TGCA'G
Cuts at: 0 107 384 443 494 1070 1098
Size: 107 277 59 51 576 28
Fragments arranged by size:
576 277 107 59 51 28
PvuII CAG'CTG
Cuts at: 0 215 262 388 1098
Size: 215 47 126 710
Fragments arranged by size:
710 215 126 47
SacI G_AGCT'C
Cuts at: 0 902 1098
Size: 902 196
SfcI C'TryA_G
Cuts at: 0 103 380 407 439 490 1066 1098
Size: 103 277 27 32 51 576 32
Fragments arranged by size:
576 277 103 51 32 32 27
SmaI CCC'GGG
Cuts at: 0 697 1098
Size: 697 401
SmlI C'TyrA_G
Cuts at: 0 1075 1098
Size: 1075 23
SphI G_CATG'C
Cuts at: 0 300 1098
Size: 300 798
StuI AGG'CCT
Cuts at: 0 916 1098
Size: 916 182
StyI C'CwwG_G
Cuts at: 0 431 1098
Size: 431 667
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 850 1006 1098
Size: 850 156 92
Fragments arranged by size:
850 156 92
TatI w'GTAC_w
Cuts at: 0 530 1098
Size: 530 568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 736 788 1098
Size: 736 52 310
Fragments arranged by size:
736 310 52
XmaI C'CCGG_G
Cuts at: 0 695 1098
Size: 695 403
Enzymes that do cut:
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI
BbvCI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BsrDI BsrFI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PspOMI PstI
PvuII SacI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BglI BglII BmtI BsaI BsaAI BsaBI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstZ17I BtgZI ClaI CspCI DraI EagI EciI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NarI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI