Restriction map for
pF1KB6534
(Linear) (Six-base) MAPSORT of: KIBB6534.seq Check: 8402 from: 1 to: 1170
KIBB6534.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 512 1170
Size: 322 190 658
Fragments arranged by size:
658 322 190
AflIII A'CryG_T
Cuts at: 0 832 834 890 1170
Size: 832 2 56 280
Fragments arranged by size:
832 280 56 2
AlwNI CAG_nnn'CTG
Cuts at: 0 1027 1170
Size: 1027 143
ApoI r'AATT_y
Cuts at: 0 3 271 373 565 595 1170
Size: 3 268 102 192 30 575
Fragments arranged by size:
575 268 192 102 30 3
BclI T'GATC_A
Cuts at: 0 177 1170
Size: 177 993
BfrBI ATG'CAT
Cuts at: 0 292 1170
Size: 292 878
BglII A'GATC_T
Cuts at: 0 717 1170
Size: 717 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 691 745 998 1170
Size: 401 290 54 253 172
Fragments arranged by size:
401 290 253 172 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1170
Size: 868 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 763 970 1170
Size: 763 207 200
Fragments arranged by size:
763 207 200
BsaAI yAC'GTr
Cuts at: 0 835 1170
Size: 835 335
BsaBI GATnn'nnATC
Cuts at: 0 1056 1161 1170
Size: 1056 105 9
Fragments arranged by size:
1056 105 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 948 978 1170
Size: 948 30 192
Fragments arranged by size:
948 192 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1027 1170
Size: 1027 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1170
Size: 131 1039
BsrGI T'GTAC_A
Cuts at: 0 678 1170
Size: 678 492
BssSI C'ACGA_G
Cuts at: 0 967 1170
Size: 967 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1027 1170
Size: 1027 143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 909 1170
Size: 693 216 261
Fragments arranged by size:
693 261 216
BstYI r'GATC_y
Cuts at: 0 717 1170
Size: 717 453
BtgI C'CryG_G
Cuts at: 0 902 960 1170
Size: 902 58 210
Fragments arranged by size:
902 210 58
BtsI GCAGTG_nn'
Cuts at: 0 143 559 782 1170
Size: 143 416 223 388
Fragments arranged by size:
416 388 223 143
ClaI AT'CG_AT
Cuts at: 0 752 1170
Size: 752 418
DraIII CAC_nnn'GTG
Cuts at: 0 13 1170
Size: 13 1157
EaeI y'GGCC_r
Cuts at: 0 601 1170
Size: 601 569
EarI CTCTTCn'nnn_
Cuts at: 0 325 767 863 1076 1150 1170
Size: 325 442 96 213 74 20
Fragments arranged by size:
442 325 213 96 74 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 401 512 691 745 998 1170
Size: 322 79 111 179 54 253 172
Fragments arranged by size:
322 253 179 172 111 79 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 988 1170
Size: 988 182
EcoO109I rG'GnC_Cy
Cuts at: 0 690 1170
Size: 690 480
EcoRI G'AATT_C
Cuts at: 0 3 271 1170
Size: 3 268 899
Fragments arranged by size:
899 268 3
EcoRV GAT'ATC
Cuts at: 0 918 1170
Size: 918 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 948 980 1170
Size: 948 32 190
Fragments arranged by size:
948 190 32
HindIII A'AGCT_T
Cuts at: 0 259 766 1170
Size: 259 507 404
Fragments arranged by size:
507 404 259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 1170
Size: 72 1098
MscI TGG'CCA
Cuts at: 0 603 1170
Size: 603 567
MslI CAynn'nnrTG
Cuts at: 0 631 730 907 1170
Size: 631 99 177 263
Fragments arranged by size:
631 263 177 99
MspA1I CmG'CkG
Cuts at: 0 962 1033 1042 1170
Size: 962 71 9 128
Fragments arranged by size:
962 128 71 9
NcoI C'CATG_G
Cuts at: 0 902 1170
Size: 902 268
NdeI CA'TA_TG
Cuts at: 0 294 1170
Size: 294 876
NsiI A_TGCA'T
Cuts at: 0 294 1170
Size: 294 876
PfoI T'CCnGG_A
Cuts at: 0 692 1170
Size: 692 478
PmlI CAC'GTG
Cuts at: 0 835 1170
Size: 835 335
PpuMI rG'GwC_Cy
Cuts at: 0 690 1170
Size: 690 480
PshAI GACnn'nnGTC
Cuts at: 0 835 1170
Size: 835 335
PstI C_TGCA'G
Cuts at: 0 1032 1170
Size: 1032 138
PvuII CAG'CTG
Cuts at: 0 1033 1170
Size: 1033 137
SacII CC_GC'GG
Cuts at: 0 963 1170
Size: 963 207
SapI GCTCTTCn'nnn_
Cuts at: 0 325 1170
Size: 325 845
SfcI C'TryA_G
Cuts at: 0 1028 1043 1170
Size: 1028 15 127
Fragments arranged by size:
1028 127 15
SmlI C'TyrA_G
Cuts at: 0 883 1170
Size: 883 287
SspI AAT'ATT
Cuts at: 0 355 501 1170
Size: 355 146 669
Fragments arranged by size:
669 355 146
StuI AGG'CCT
Cuts at: 0 994 1170
Size: 994 176
StyI C'CwwG_G
Cuts at: 0 686 902 1170
Size: 686 216 268
Fragments arranged by size:
686 268 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 1170
Size: 438 732
TatI w'GTAC_w
Cuts at: 0 678 1170
Size: 678 492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 1170
Size: 909 261
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BclI BfrBI BglII BpmI
BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsgI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtsI ClaI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NdeI NsiI
PfoI PmlI PpuMI PshAI PstI PvuII SacII SapI
SfcI SmlI SspI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DraI DrdI EagI EciI EcoICRI FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PflMI PmeI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI