Restriction map for
pF1KB6537
(Linear) (Six-base) MAPSORT of: KIBB6537.seq Check: 4974 from: 1 to: 1101
KIBB6537.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AfeI AGC'GCT
Cuts at: 0 106 394 982 1101
Size: 106 288 588 119
Fragments arranged by size:
588 288 119 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 125 1082 1101
Size: 125 957 19
Fragments arranged by size:
957 125 19
ApoI r'AATT_y
Cuts at: 0 784 850 1101
Size: 784 66 251
Fragments arranged by size:
784 251 66
AvaI C'yCGr_G
Cuts at: 0 278 463 845 1101
Size: 278 185 382 256
Fragments arranged by size:
382 278 256 185
BanI G'GyrC_C
Cuts at: 0 51 1101
Size: 51 1050
BanII G_rGCy'C
Cuts at: 0 285 350 689 958 1101
Size: 285 65 339 269 143
Fragments arranged by size:
339 285 269 143 65
BbeI G_GCGC'C
Cuts at: 0 55 1101
Size: 55 1046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1101
Size: 1038 63
BsaAI yAC'GTr
Cuts at: 0 317 423 1101
Size: 317 106 678
Fragments arranged by size:
678 317 106
BsaHI Gr'CG_yC
Cuts at: 0 52 1101
Size: 52 1049
BsaWI w'CCGG_w
Cuts at: 0 482 882 1101
Size: 482 400 219
Fragments arranged by size:
482 400 219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 760 790 826 856 1101
Size: 760 30 36 30 245
Fragments arranged by size:
760 245 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 850 1101
Size: 54 796 251
Fragments arranged by size:
796 251 54
BseYI C'CCAG_C
Cuts at: 0 958 1041 1101
Size: 958 83 60
Fragments arranged by size:
958 83 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 131 1101
Size: 121 10 970
Fragments arranged by size:
970 121 10
BsiHKAI G_wGCw'C
Cuts at: 0 285 350 689 894 1101
Size: 285 65 339 205 207
Fragments arranged by size:
339 285 207 205 65
BsmI GAATG_Cn'
Cuts at: 0 447 1101
Size: 447 654
Bsp1286I G_dGCh'C
Cuts at: 0 285 350 689 894 958 1101
Size: 285 65 339 205 64 143
Fragments arranged by size:
339 285 205 143 65 64
BspEI T'CCGG_A
Cuts at: 0 882 1101
Size: 882 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 1101
Size: 668 433
BtsI GCAGTG_nn'
Cuts at: 0 119 345 411 537 1101
Size: 119 226 66 126 564
Fragments arranged by size:
564 226 126 119 66
EaeI y'GGCC_r
Cuts at: 0 473 1101
Size: 473 628
EarI CTCTTCn'nnn_
Cuts at: 0 31 1101
Size: 31 1070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 669 1101
Size: 669 432
EcoICRI GAG'CTC
Cuts at: 0 283 348 687 1101
Size: 283 65 339 414
Fragments arranged by size:
414 339 283 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1101
Size: 1038 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 177 1101
Size: 177 924
EcoRI G'AATT_C
Cuts at: 0 850 1101
Size: 850 251
EcoRV GAT'ATC
Cuts at: 0 1080 1101
Size: 1080 21
HaeII r_GCGC'y
Cuts at: 0 55 108 396 984 1101
Size: 55 53 288 588 117
Fragments arranged by size:
588 288 117 55 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 826 858 1101
Size: 760 32 34 32 243
Fragments arranged by size:
760 243 34 32 32
HincII GTy'rAC
Cuts at: 0 159 738 1101
Size: 159 579 363
Fragments arranged by size:
579 363 159
KasI G'GCGC_C
Cuts at: 0 51 1101
Size: 51 1050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 1101
Size: 601 500
MslI CAynn'nnrTG
Cuts at: 0 376 1101
Size: 376 725
NarI GG'CG_CC
Cuts at: 0 52 1101
Size: 52 1049
NdeI CA'TA_TG
Cuts at: 0 902 1101
Size: 902 199
NruI TCG'CGA
Cuts at: 0 977 1101
Size: 977 124
NspI r_CATG'y
Cuts at: 0 570 1101
Size: 570 531
PsiI TTA'TAA
Cuts at: 0 545 1101
Size: 545 556
PstI C_TGCA'G
Cuts at: 0 665 935 1071 1101
Size: 665 270 136 30
Fragments arranged by size:
665 270 136 30
SacI G_AGCT'C
Cuts at: 0 285 350 689 1101
Size: 285 65 339 412
Fragments arranged by size:
412 339 285 65
SfcI C'TryA_G
Cuts at: 0 661 931 1067 1101
Size: 661 270 136 34
Fragments arranged by size:
661 270 136 34
SfoI GGC'GCC
Cuts at: 0 53 1101
Size: 53 1048
SnaBI TAC'GTA
Cuts at: 0 317 1101
Size: 317 784
SspI AAT'ATT
Cuts at: 0 251 1101
Size: 251 850
StyI C'CwwG_G
Cuts at: 0 20 1101
Size: 20 1081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 452 484 1101
Size: 27 425 32 617
Fragments arranged by size:
617 425 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 403 1101
Size: 403 698
XmnI GAAnn'nnTTC
Cuts at: 0 304 788 1101
Size: 304 484 313
Fragments arranged by size:
484 313 304
Enzymes that do cut:
AfeI AlfI ApoI AvaI BanI BanII BbeI BpmI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV HaeII Hin4I HincII
KasI MmeI MslI NarI NdeI NruI NspI PsiI
PstI SacI SfcI SfoI SnaBI SspI StyI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsiEI
BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoO109I FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI MspA1I NaeI NcoI NgoMIV NheI
NotI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StuI SwaI TaqII TatI XbaI XcmI XhoI
XmaI ZraI