Restriction map for pF1KB6538
 (Linear) (Six-base) MAPSORT of: KIBB6538.seq  Check: 1138  from: 1  to: 1173



KIBB6538.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     512    1173 
   Size:        512     661
 
AhdI GACnn_n'nnGTC

Cuts at:      0     387    1173 
   Size:        387     786
 
AlwNI CAG_nnn'CTG

Cuts at:      0     207     618     822    1057    1173 
   Size:        207     411     204     235     116

  Fragments arranged by size: 

                 411     235     207     204     116
 
ApaI G_GGCC'C

Cuts at:      0    1135    1173 
   Size:       1135      38
 
ApoI r'AATT_y

Cuts at:      0     661    1173 
   Size:        661     512
 
BanII G_rGCy'C

Cuts at:      0      80     107     674     829     955    1009    1046    1135 
   Size:         80      27     567     155     126      54      37      89

Cuts at:   1135    1173 
   Size:         38

  Fragments arranged by size: 

                 567     155     126      89      80      54      38      37
                  27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     240     280     364     396    1160    1173 
   Size:        240      40      84      32     764      13

  Fragments arranged by size: 

                 764     240      84      40      32      13
 
BbvCI CC'TCA_GC

Cuts at:      0     854    1173 
   Size:        854     319
 
BlpI GC'TnA_GC

Cuts at:      0    1040    1173 
   Size:       1040     133
 
Bme1580I G_kGCm'C

Cuts at:      0     424    1135    1173 
   Size:        424     711      38

  Fragments arranged by size: 

                 711     424      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1040    1173 
   Size:       1040     133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     710     844    1173 
   Size:        289     421     134     329

  Fragments arranged by size: 

                 421     329     289     134
 
Bpu10I CC'TnA_GC

Cuts at:      0     676     854    1173 
   Size:        676     178     319

  Fragments arranged by size: 

                 676     319     178
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    1173 
   Size:        640     533
 
BsaWI w'CCGG_w

Cuts at:      0     113    1173 
   Size:        113    1060
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     796     811     826     841    1173 
   Size:         91      30     675      15      15      15     332

  Fragments arranged by size: 

                 675     332      91      30      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     117     562     565     616    1039    1173 
   Size:        117     445       3      51     423     134

  Fragments arranged by size: 

                 445     423     134     117      51       3
 
BseYI C'CCAG_C

Cuts at:      0     411     441     501     992    1135    1173 
   Size:        411      30      60     491     143      38

  Fragments arranged by size: 

                 491     411     143      60      38      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    1173 
   Size:        467     706
 
BsiHKAI G_wGCw'C

Cuts at:      0      38      80    1173 
   Size:         38      42    1093

  Fragments arranged by size: 

                1093      42      38
 
BsmI GAATG_Cn'

Cuts at:      0     710    1173 
   Size:        710     463
 
Bsp1286I G_dGCh'C

Cuts at:      0      38      80     107     424     674     829     955    1009 
   Size:         38      42      27     317     250     155     126      54

Cuts at:   1009    1046    1135    1173 
   Size:         37      89      38

  Fragments arranged by size: 

                 317     250     155     126      89      54      42      38
                  38      37      27
 
BspEI T'CCGG_A

Cuts at:      0     113    1173 
   Size:        113    1060
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     215    1173 
   Size:        215     958
 
BsrBI CCG'CTC

Cuts at:      0     134     165     254    1085    1173 
   Size:        134      31      89     831      88

  Fragments arranged by size: 

                 831     134      89      88      31
 
BsrDI GCAATG_nn'

Cuts at:      0     336     951    1173 
   Size:        336     615     222

  Fragments arranged by size: 

                 615     336     222
 
BssSI C'ACGA_G

Cuts at:      0     417     544    1173 
   Size:        417     127     629

  Fragments arranged by size: 

                 629     417     127
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     576    1173 
   Size:        576     597
 
BstYI r'GATC_y

Cuts at:      0     221    1173 
   Size:        221     952
 
BtgI C'CryG_G

Cuts at:      0     513     575     926    1173 
   Size:        513      62     351     247

  Fragments arranged by size: 

                 513     351     247      62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     277    1173 
   Size:        277     896
 
BtsI GCAGTG_nn'

Cuts at:      0     563    1173 
   Size:        563     610
 
EaeI y'GGCC_r

Cuts at:      0     306     510     516     638     771     785    1173 
   Size:        306     204       6     122     133      14     388

  Fragments arranged by size: 

                 388     306     204     133     122      14       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     172    1173 
   Size:        172    1001
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1165    1173 
   Size:       1165       8
 
EcoICRI GAG'CTC

Cuts at:      0      78    1173 
   Size:         78    1095
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     512     710     844    1173 
   Size:        289     223     198     134     329

  Fragments arranged by size: 

                 329     289     223     198     134
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     598    1173 
   Size:        598     575
 
EcoO109I rG'GnC_Cy

Cuts at:      0     204     819    1131    1132    1173 
   Size:        204     615     312       1      41

  Fragments arranged by size: 

                 615     312     204      41       1
 
EcoRI G'AATT_C

Cuts at:      0     661    1173 
   Size:        661     512
 
FspI TGC'GCA

Cuts at:      0     535    1173 
   Size:        535     638
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     373     400     405     432     790     796 
   Size:         91      32     250      27       5      27     358       6

Cuts at:    796     811     822     828     843     895     927     996    1028 
   Size:         15      11       6      15      52      32      69      32

Cuts at:   1028    1173 
   Size:        145

  Fragments arranged by size: 

                 358     250     145      91      69      52      32      32
                  32      27      27      15      15      11       6       6
                   5
 
MscI TGG'CCA

Cuts at:      0     640     787    1173 
   Size:        640     147     386

  Fragments arranged by size: 

                 640     386     147
 
MslI CAynn'nnrTG

Cuts at:      0     719    1173 
   Size:        719     454
 
MspA1I CmG'CkG

Cuts at:      0     333     618    1173 
   Size:        333     285     555

  Fragments arranged by size: 

                 555     333     285
 
NcoI C'CATG_G

Cuts at:      0     575     926    1173 
   Size:        575     351     247

  Fragments arranged by size: 

                 575     351     247
 
NspI r_CATG'y

Cuts at:      0     432    1173 
   Size:        432     741
 
PasI CC'CwG_GG

Cuts at:      0    1128    1173 
   Size:       1128      45
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     822    1173 
   Size:        822     351
 
PfoI T'CCnGG_A

Cuts at:      0     377    1173 
   Size:        377     796
 
PpuMI rG'GwC_Cy

Cuts at:      0     204     819    1173 
   Size:        204     615     354

  Fragments arranged by size: 

                 615     354     204
 
PspOMI G'GGCC_C

Cuts at:      0    1131    1173 
   Size:       1131      42
 
PstI C_TGCA'G

Cuts at:      0     398     623    1173 
   Size:        398     225     550

  Fragments arranged by size: 

                 550     398     225
 
PvuII CAG'CTG

Cuts at:      0     333     618    1173 
   Size:        333     285     555

  Fragments arranged by size: 

                 555     333     285
 
SacI G_AGCT'C

Cuts at:      0      80    1173 
   Size:         80    1093
 
SapI GCTCTTCn'nnn_

Cuts at:      0     172    1173 
   Size:        172    1001
 
SbfI CC_TGCA'GG

Cuts at:      0     398    1173 
   Size:        398     775
 
SfcI C'TryA_G

Cuts at:      0     394     619     938    1173 
   Size:        394     225     319     235

  Fragments arranged by size: 

                 394     319     235     225
 
SmlI C'TyrA_G

Cuts at:      0     655    1173 
   Size:        655     518
 
StyI C'CwwG_G

Cuts at:      0      92     575     594     926    1173 
   Size:         92     483      19     332     247

  Fragments arranged by size: 

                 483     332     247      92      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1086    1173 
   Size:       1086      87
 
TatI w'GTAC_w

Cuts at:      0    1048    1173 
   Size:       1048     125
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      43      75     355     387    1173 
   Size:         43      32     280      32     786

  Fragments arranged by size: 

                 786     280      43      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1143    1173 
   Size:       1143      30
 
XmnI GAAnn'nnTTC

Cuts at:      0     235    1173 
   Size:        235     938
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApaI     ApoI    BanII     BbsI    BbvCI
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FspI    Hin4I     MscI     MslI   MspA1I     NcoI     NspI     PasI
    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     SmlI     StyI    TaqII     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI    BsmBI    BspHI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI     XmaI     ZraI
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