(Linear) (Six-base) MAPSORT of: KIBB6538.seq Check: 1138 from: 1 to: 1173 KIBB6538.orf With 182 enzymes: SgfI * October 24, 2019 20:49 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 512 1173 Size: 512 661 AhdI GACnn_n'nnGTC Cuts at: 0 387 1173 Size: 387 786 AlwNI CAG_nnn'CTG Cuts at: 0 207 618 822 1057 1173 Size: 207 411 204 235 116 Fragments arranged by size: 411 235 207 204 116 ApaI G_GGCC'C Cuts at: 0 1135 1173 Size: 1135 38 ApoI r'AATT_y Cuts at: 0 661 1173 Size: 661 512 BanII G_rGCy'C Cuts at: 0 80 107 674 829 955 1009 1046 1135 Size: 80 27 567 155 126 54 37 89 Cuts at: 1135 1173 Size: 38 Fragments arranged by size: 567 155 126 89 80 54 38 37 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 240 280 364 396 1160 1173 Size: 240 40 84 32 764 13 Fragments arranged by size: 764 240 84 40 32 13 BbvCI CC'TCA_GC Cuts at: 0 854 1173 Size: 854 319 BlpI GC'TnA_GC Cuts at: 0 1040 1173 Size: 1040 133 Bme1580I G_kGCm'C Cuts at: 0 424 1135 1173 Size: 424 711 38 Fragments arranged by size: 711 424 38 BmrI ACTGGGnnnn_n' Cuts at: 0 1040 1173 Size: 1040 133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 710 844 1173 Size: 289 421 134 329 Fragments arranged by size: 421 329 289 134 Bpu10I CC'TnA_GC Cuts at: 0 676 854 1173 Size: 676 178 319 Fragments arranged by size: 676 319 178 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1173 Size: 640 533 BsaWI w'CCGG_w Cuts at: 0 113 1173 Size: 113 1060 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 91 121 796 811 826 841 1173 Size: 91 30 675 15 15 15 332 Fragments arranged by size: 675 332 91 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 117 562 565 616 1039 1173 Size: 117 445 3 51 423 134 Fragments arranged by size: 445 423 134 117 51 3 BseYI C'CCAG_C Cuts at: 0 411 441 501 992 1135 1173 Size: 411 30 60 491 143 38 Fragments arranged by size: 491 411 143 60 38 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 467 1173 Size: 467 706 BsiHKAI G_wGCw'C Cuts at: 0 38 80 1173 Size: 38 42 1093 Fragments arranged by size: 1093 42 38 BsmI GAATG_Cn' Cuts at: 0 710 1173 Size: 710 463 Bsp1286I G_dGCh'C Cuts at: 0 38 80 107 424 674 829 955 1009 Size: 38 42 27 317 250 155 126 54 Cuts at: 1009 1046 1135 1173 Size: 37 89 38 Fragments arranged by size: 317 250 155 126 89 54 42 38 38 37 27 BspEI T'CCGG_A Cuts at: 0 113 1173 Size: 113 1060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 215 1173 Size: 215 958 BsrBI CCG'CTC Cuts at: 0 134 165 254 1085 1173 Size: 134 31 89 831 88 Fragments arranged by size: 831 134 89 88 31 BsrDI GCAATG_nn' Cuts at: 0 336 951 1173 Size: 336 615 222 Fragments arranged by size: 615 336 222 BssSI C'ACGA_G Cuts at: 0 417 544 1173 Size: 417 127 629 Fragments arranged by size: 629 417 127 BstXI CCAn_nnnn'nTGG Cuts at: 0 576 1173 Size: 576 597 BstYI r'GATC_y Cuts at: 0 221 1173 Size: 221 952 BtgI C'CryG_G Cuts at: 0 513 575 926 1173 Size: 513 62 351 247 Fragments arranged by size: 513 351 247 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 277 1173 Size: 277 896 BtsI GCAGTG_nn' Cuts at: 0 563 1173 Size: 563 610 EaeI y'GGCC_r Cuts at: 0 306 510 516 638 771 785 1173 Size: 306 204 6 122 133 14 388 Fragments arranged by size: 388 306 204 133 122 14 6 EarI CTCTTCn'nnn_ Cuts at: 0 172 1173 Size: 172 1001 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1165 1173 Size: 1165 8 EcoICRI GAG'CTC Cuts at: 0 78 1173 Size: 78 1095 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 512 710 844 1173 Size: 289 223 198 134 329 Fragments arranged by size: 329 289 223 198 134 EcoNI CCTnn'n_nnAGG Cuts at: 0 598 1173 Size: 598 575 EcoO109I rG'GnC_Cy Cuts at: 0 204 819 1131 1132 1173 Size: 204 615 312 1 41 Fragments arranged by size: 615 312 204 41 1 EcoRI G'AATT_C Cuts at: 0 661 1173 Size: 661 512 FspI TGC'GCA Cuts at: 0 535 1173 Size: 535 638 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 91 123 373 400 405 432 790 796 Size: 91 32 250 27 5 27 358 6 Cuts at: 796 811 822 828 843 895 927 996 1028 Size: 15 11 6 15 52 32 69 32 Cuts at: 1028 1173 Size: 145 Fragments arranged by size: 358 250 145 91 69 52 32 32 32 27 27 15 15 11 6 6 5 MscI TGG'CCA Cuts at: 0 640 787 1173 Size: 640 147 386 Fragments arranged by size: 640 386 147 MslI CAynn'nnrTG Cuts at: 0 719 1173 Size: 719 454 MspA1I CmG'CkG Cuts at: 0 333 618 1173 Size: 333 285 555 Fragments arranged by size: 555 333 285 NcoI C'CATG_G Cuts at: 0 575 926 1173 Size: 575 351 247 Fragments arranged by size: 575 351 247 NspI r_CATG'y Cuts at: 0 432 1173 Size: 432 741 PasI CC'CwG_GG Cuts at: 0 1128 1173 Size: 1128 45 PflMI CCAn_nnn'nTGG Cuts at: 0 822 1173 Size: 822 351 PfoI T'CCnGG_A Cuts at: 0 377 1173 Size: 377 796 PpuMI rG'GwC_Cy Cuts at: 0 204 819 1173 Size: 204 615 354 Fragments arranged by size: 615 354 204 PspOMI G'GGCC_C Cuts at: 0 1131 1173 Size: 1131 42 PstI C_TGCA'G Cuts at: 0 398 623 1173 Size: 398 225 550 Fragments arranged by size: 550 398 225 PvuII CAG'CTG Cuts at: 0 333 618 1173 Size: 333 285 555 Fragments arranged by size: 555 333 285 SacI G_AGCT'C Cuts at: 0 80 1173 Size: 80 1093 SapI GCTCTTCn'nnn_ Cuts at: 0 172 1173 Size: 172 1001 SbfI CC_TGCA'GG Cuts at: 0 398 1173 Size: 398 775 SfcI C'TryA_G Cuts at: 0 394 619 938 1173 Size: 394 225 319 235 Fragments arranged by size: 394 319 235 225 SmlI C'TyrA_G Cuts at: 0 655 1173 Size: 655 518 StyI C'CwwG_G Cuts at: 0 92 575 594 926 1173 Size: 92 483 19 332 247 Fragments arranged by size: 483 332 247 92 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1086 1173 Size: 1086 87 TatI w'GTAC_w Cuts at: 0 1048 1173 Size: 1048 125 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 43 75 355 387 1173 Size: 43 32 280 32 786 Fragments arranged by size: 786 280 43 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1143 1173 Size: 1143 30 XmnI GAAnn'nnTTC Cuts at: 0 235 1173 Size: 235 938 Enzymes that do cut: AcuI AhdI AlwNI ApaI ApoI BanII BbsI BbvCI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI Hin4I MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SbfI SfcI SmlI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI