Restriction map for
pF1KB6541
(Linear) (Six-base) MAPSORT of: KIBB6541.seq Check: 4351 from: 1 to: 1083
KIBB6541.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 0 654 805 1083
Size: 654 151 278
Fragments arranged by size:
654 278 151
Acc65I G'GTAC_C
Cuts at: 0 389 1083
Size: 389 694
AflIII A'CryG_T
Cuts at: 0 241 800 1083
Size: 241 559 283
Fragments arranged by size:
559 283 241
AleI CACnn'nnGTG
Cuts at: 0 254 1083
Size: 254 829
ApaI G_GGCC'C
Cuts at: 0 862 1083
Size: 862 221
ApoI r'AATT_y
Cuts at: 0 957 1026 1083
Size: 957 69 57
Fragments arranged by size:
957 69 57
AvaI C'yCGr_G
Cuts at: 0 53 1083
Size: 53 1030
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 373 406 1083
Size: 373 33 677
Fragments arranged by size:
677 373 33
BamHI G'GATC_C
Cuts at: 0 480 1083
Size: 480 603
BanI G'GyrC_C
Cuts at: 0 389 552 1083
Size: 389 163 531
Fragments arranged by size:
531 389 163
BanII G_rGCy'C
Cuts at: 0 105 862 1083
Size: 105 757 221
Fragments arranged by size:
757 221 105
BbvCI CC'TCA_GC
Cuts at: 0 699 1083
Size: 699 384
BciVI GTATCCnnnnn_n'
Cuts at: 0 413 1083
Size: 413 670
Bme1580I G_kGCm'C
Cuts at: 0 557 862 997 1083
Size: 557 305 135 86
Fragments arranged by size:
557 305 135 86
BmgBI CAC'GTC
Cuts at: 0 235 1083
Size: 235 848
BmrI ACTGGGnnnn_n'
Cuts at: 0 293 464 1083
Size: 293 171 619
Fragments arranged by size:
619 293 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 551 1074 1083
Size: 551 523 9
Fragments arranged by size:
551 523 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 416 713 1083
Size: 209 207 297 370
Fragments arranged by size:
370 297 209 207
Bpu10I CC'TnA_GC
Cuts at: 0 699 930 1083
Size: 699 231 153
Fragments arranged by size:
699 231 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1083
Size: 237 846
BsaI GGTCTCn'nnnn_
Cuts at: 0 582 1083
Size: 582 501
BsaAI yAC'GTr
Cuts at: 0 75 1083
Size: 75 1008
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 527 687 1083
Size: 527 160 396
Fragments arranged by size:
527 396 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1083
Size: 239 844
BsiHKAI G_wGCw'C
Cuts at: 0 105 1083
Size: 105 978
BsmI GAATG_Cn'
Cuts at: 0 606 626 822 1083
Size: 606 20 196 261
Fragments arranged by size:
606 261 196 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 225 1083
Size: 225 858
Bsp1286I G_dGCh'C
Cuts at: 0 105 557 862 997 1083
Size: 105 452 305 135 86
Fragments arranged by size:
452 305 135 105 86
BsrDI GCAATG_nn'
Cuts at: 0 590 1083
Size: 590 493
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 1083
Size: 477 606
BstYI r'GATC_y
Cuts at: 0 207 480 1083
Size: 207 273 603
Fragments arranged by size:
603 273 207
BstZ17I GTA'TAC
Cuts at: 0 806 1083
Size: 806 277
Bsu36I CC'TnA_GG
Cuts at: 0 558 1083
Size: 558 525
BtgI C'CryG_G
Cuts at: 0 878 1083
Size: 878 205
EaeI y'GGCC_r
Cuts at: 0 661 1083
Size: 661 422
EcoICRI GAG'CTC
Cuts at: 0 103 1083
Size: 103 980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 416 713 1083
Size: 209 207 297 370
Fragments arranged by size:
370 297 209 207
EcoNI CCTnn'n_nnAGG
Cuts at: 0 542 731 1083
Size: 542 189 352
Fragments arranged by size:
542 352 189
EcoO109I rG'GnC_Cy
Cuts at: 0 346 407 724 859 1083
Size: 346 61 317 135 224
Fragments arranged by size:
346 317 224 135 61
EcoRI G'AATT_C
Cuts at: 0 957 1083
Size: 957 126
HaeII r_GCGC'y
Cuts at: 0 32 1083
Size: 32 1051
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 411 443 1083
Size: 411 32 640
Fragments arranged by size:
640 411 32
KpnI G_GTAC'C
Cuts at: 0 393 1083
Size: 393 690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 508 1083
Size: 65 443 575
Fragments arranged by size:
575 443 65
MscI TGG'CCA
Cuts at: 0 663 1083
Size: 663 420
MslI CAynn'nnrTG
Cuts at: 0 254 914 1072 1083
Size: 254 660 158 11
Fragments arranged by size:
660 254 158 11
NcoI C'CATG_G
Cuts at: 0 878 1083
Size: 878 205
NspI r_CATG'y
Cuts at: 0 245 339 804 1083
Size: 245 94 465 279
Fragments arranged by size:
465 279 245 94
PasI CC'CwG_GG
Cuts at: 0 862 1083
Size: 862 221
PciI A'CATG_T
Cuts at: 0 241 800 1083
Size: 241 559 283
Fragments arranged by size:
559 283 241
PflMI CCAn_nnn'nTGG
Cuts at: 0 331 649 736 1083
Size: 331 318 87 347
Fragments arranged by size:
347 331 318 87
PfoI T'CCnGG_A
Cuts at: 0 729 828 1083
Size: 729 99 255
Fragments arranged by size:
729 255 99
PpuMI rG'GwC_Cy
Cuts at: 0 346 724 1083
Size: 346 378 359
Fragments arranged by size:
378 359 346
PspOMI G'GGCC_C
Cuts at: 0 858 1083
Size: 858 225
PstI C_TGCA'G
Cuts at: 0 291 1083
Size: 291 792
SacI G_AGCT'C
Cuts at: 0 105 1083
Size: 105 978
SfcI C'TryA_G
Cuts at: 0 287 1083
Size: 287 796
SmaI CCC'GGG
Cuts at: 0 55 1083
Size: 55 1028
SmlI C'TyrA_G
Cuts at: 0 252 1083
Size: 252 831
SnaBI TAC'GTA
Cuts at: 0 75 1083
Size: 75 1008
SphI G_CATG'C
Cuts at: 0 339 1083
Size: 339 744
StyI C'CwwG_G
Cuts at: 0 403 658 878 1000 1083
Size: 403 255 220 122 83
Fragments arranged by size:
403 255 220 122 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 502 771 871 1083
Size: 502 269 100 212
Fragments arranged by size:
502 269 212 100
XmaI C'CCGG_G
Cuts at: 0 53 1083
Size: 53 1030
Enzymes that do cut:
AccI Acc65I AflIII AleI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbvCI BciVI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BsrDI BstXI BstYI BstZ17I
Bsu36I BtgI EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KpnI MmeI MscI MslI NcoI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PstI SacI
SfcI SmaI SmlI SnaBI SphI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BbeI BbsI BcgI BclI BfrBI BglI BglII BlpI
BmtI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SpeI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI