Restriction map for
pF1KB6542
(Linear) (Six-base) MAPSORT of: KIBB6542.seq Check: 3235 from: 1 to: 1173
KIBB6542.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 0 437 1173
Size: 437 736
AleI CACnn'nnGTG
Cuts at: 0 251 1173
Size: 251 922
ApoI r'AATT_y
Cuts at: 0 285 571 654 1173
Size: 285 286 83 519
Fragments arranged by size:
519 286 285 83
AvaI C'yCGr_G
Cuts at: 0 260 1173
Size: 260 913
BanII G_rGCy'C
Cuts at: 0 95 413 827 1173
Size: 95 318 414 346
Fragments arranged by size:
414 346 318 95
BbvCI CC'TCA_GC
Cuts at: 0 404 1173
Size: 404 769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 395 1173
Size: 361 34 778
Fragments arranged by size:
778 361 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 201 1173
Size: 201 972
Bme1580I G_kGCm'C
Cuts at: 0 816 1173
Size: 816 357
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 530 1173
Size: 530 643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 106 538 947 1173
Size: 52 54 432 409 226
Fragments arranged by size:
432 409 226 54 52
Bpu10I CC'TnA_GC
Cuts at: 0 404 1173
Size: 404 769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1173
Size: 100 1073
BsaI GGTCTCn'nnnn_
Cuts at: 0 501 1173
Size: 501 672
BsaBI GATnn'nnATC
Cuts at: 0 1037 1173
Size: 1037 136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 421 1173
Size: 103 318 752
Fragments arranged by size:
752 318 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1173
Size: 28 1145
BsiHKAI G_wGCw'C
Cuts at: 0 95 374 413 1173
Size: 95 279 39 760
Fragments arranged by size:
760 279 95 39
BsmI GAATG_Cn'
Cuts at: 0 395 1173
Size: 395 778
Bsp1286I G_dGCh'C
Cuts at: 0 95 374 413 816 827 1173
Size: 95 279 39 403 11 346
Fragments arranged by size:
403 346 279 95 39 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 519 853 1173
Size: 519 334 320
Fragments arranged by size:
519 334 320
BsrFI r'CCGG_y
Cuts at: 0 106 1173
Size: 106 1067
BssSI C'ACGA_G
Cuts at: 0 121 1173
Size: 121 1052
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1053 1173
Size: 1053 120
BstYI r'GATC_y
Cuts at: 0 125 1173
Size: 125 1048
BtgI C'CryG_G
Cuts at: 0 138 830 1173
Size: 138 692 343
Fragments arranged by size:
692 343 138
BtsI GCAGTG_nn'
Cuts at: 0 258 312 533 925 1173
Size: 258 54 221 392 248
Fragments arranged by size:
392 258 248 221 54
EaeI y'GGCC_r
Cuts at: 0 141 1173
Size: 141 1032
EcoICRI GAG'CTC
Cuts at: 0 93 411 1173
Size: 93 318 762
Fragments arranged by size:
762 318 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 106 538 947 1173
Size: 52 54 432 409 226
Fragments arranged by size:
432 409 226 54 52
EcoRV GAT'ATC
Cuts at: 0 783 1173
Size: 783 390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 985 1173
Size: 985 188
HaeII r_GCGC'y
Cuts at: 0 877 1173
Size: 877 296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 254 1173
Size: 246 8 919
Fragments arranged by size:
919 246 8
MslI CAynn'nnrTG
Cuts at: 0 251 1173
Size: 251 922
MspA1I CmG'CkG
Cuts at: 0 69 1173
Size: 69 1104
NdeI CA'TA_TG
Cuts at: 0 747 1173
Size: 747 426
PacI TTA_AT'TAA
Cuts at: 0 890 1173
Size: 890 283
PfoI T'CCnGG_A
Cuts at: 0 1146 1173
Size: 1146 27
PshAI GACnn'nnGTC
Cuts at: 0 443 1173
Size: 443 730
PstI C_TGCA'G
Cuts at: 0 134 1173
Size: 134 1039
PvuII CAG'CTG
Cuts at: 0 69 1173
Size: 69 1104
SacI G_AGCT'C
Cuts at: 0 95 413 1173
Size: 95 318 760
Fragments arranged by size:
760 318 95
SfcI C'TryA_G
Cuts at: 0 130 434 1173
Size: 130 304 739
Fragments arranged by size:
739 304 130
SgrAI Cr'CCGG_yG
Cuts at: 0 106 1173
Size: 106 1067
SmlI C'TyrA_G
Cuts at: 0 115 1173
Size: 115 1058
SspI AAT'ATT
Cuts at: 0 479 1173
Size: 479 694
StyI C'CwwG_G
Cuts at: 0 566 969 1173
Size: 566 403 204
Fragments arranged by size:
566 403 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 601 1059 1173
Size: 601 458 114
Fragments arranged by size:
601 458 114
TatI w'GTAC_w
Cuts at: 0 922 1173
Size: 922 251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 698 730 1173
Size: 698 32 443
Fragments arranged by size:
698 443 32
Enzymes that do cut:
AccI AleI ApoI AvaI BanII BbvCI BcgI BciVI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BssSI BstXI
BstYI BtgI BtsI EaeI EcoICRI Eco57MI EcoRV FalI
HaeII MmeI MslI MspA1I NdeI PacI PfoI PshAI
PstI PvuII SacI SfcI SgrAI SmlI SspI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoNI EcoO109I EcoRI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI