Restriction map for
pF1KB6551
(Linear) (Six-base) MAPSORT of: KIBB6551.seq Check: 3963 from: 1 to: 1113
KIBB6551.orf
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1113
Size: 455 658
AhdI GACnn_n'nnGTC
Cuts at: 0 79 1113
Size: 79 1034
AleI CACnn'nnGTG
Cuts at: 0 629 1113
Size: 629 484
ApaI G_GGCC'C
Cuts at: 0 27 1037 1113
Size: 27 1010 76
Fragments arranged by size:
1010 76 27
ApoI r'AATT_y
Cuts at: 0 522 1113
Size: 522 591
AvaI C'yCGr_G
Cuts at: 0 53 815 851 884 950 1113
Size: 53 762 36 33 66 163
Fragments arranged by size:
762 163 66 53 36 33
BanI G'GyrC_C
Cuts at: 0 899 1113
Size: 899 214
BanII G_rGCy'C
Cuts at: 0 27 308 1037 1060 1113
Size: 27 281 729 23 53
Fragments arranged by size:
729 281 53 27 23
BbeI G_GCGC'C
Cuts at: 0 903 1113
Size: 903 210
BbvCI CC'TCA_GC
Cuts at: 0 506 1113
Size: 506 607
BclI T'GATC_A
Cuts at: 0 675 1113
Size: 675 438
BglII A'GATC_T
Cuts at: 0 841 1113
Size: 841 272
Bme1580I G_kGCm'C
Cuts at: 0 27 49 1037 1113
Size: 27 22 988 76
Fragments arranged by size:
988 76 27 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1065 1113
Size: 963 102 48
Fragments arranged by size:
963 102 48
Bpu10I CC'TnA_GC
Cuts at: 0 506 1113
Size: 506 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1072 1113
Size: 750 322 41
Fragments arranged by size:
750 322 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 227 598 1113
Size: 227 371 515
Fragments arranged by size:
515 371 227
BsaBI GATnn'nnATC
Cuts at: 0 893 1113
Size: 893 220
BsaHI Gr'CG_yC
Cuts at: 0 825 900 1113
Size: 825 75 213
Fragments arranged by size:
825 213 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 961 991 1113
Size: 961 30 122
Fragments arranged by size:
961 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 747 750 757 772 1015 1113
Size: 747 3 7 15 243 98
Fragments arranged by size:
747 243 98 15 7 3
BseYI C'CCAG_C
Cuts at: 0 148 1113
Size: 148 965
BsiHKAI G_wGCw'C
Cuts at: 0 308 1060 1113
Size: 308 752 53
Fragments arranged by size:
752 308 53
BsmBI CGTCTCn'nnnn_
Cuts at: 0 56 1113
Size: 56 1057
Bsp1286I G_dGCh'C
Cuts at: 0 27 49 308 1037 1060 1113
Size: 27 22 259 729 23 53
Fragments arranged by size:
729 259 53 27 23 22
BsrBI CCG'CTC
Cuts at: 0 987 1016 1113
Size: 987 29 97
Fragments arranged by size:
987 97 29
BsrDI GCAATG_nn'
Cuts at: 0 263 1113
Size: 263 850
BsrFI r'CCGG_y
Cuts at: 0 1102 1113
Size: 1102 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 558 1113
Size: 558 555
BstXI CCAn_nnnn'nTGG
Cuts at: 0 631 1113
Size: 631 482
BstYI r'GATC_y
Cuts at: 0 659 841 1113
Size: 659 182 272
Fragments arranged by size:
659 272 182
EarI CTCTTCn'nnn_
Cuts at: 0 706 1085 1113
Size: 706 379 28
Fragments arranged by size:
706 379 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1035 1113
Size: 1035 78
EcoICRI GAG'CTC
Cuts at: 0 306 1058 1113
Size: 306 752 55
Fragments arranged by size:
752 306 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 963 1065 1113
Size: 455 508 102 48
Fragments arranged by size:
508 455 102 48
EcoO109I rG'GnC_Cy
Cuts at: 0 24 1033 1034 1113
Size: 24 1009 1 79
Fragments arranged by size:
1009 79 24 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 660 1113
Size: 660 453
HaeII r_GCGC'y
Cuts at: 0 903 1113
Size: 903 210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 976 1008 1113
Size: 65 32 879 32 105
Fragments arranged by size:
879 105 65 32 32
HindIII A'AGCT_T
Cuts at: 0 670 1113
Size: 670 443
KasI G'GCGC_C
Cuts at: 0 899 1113
Size: 899 214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1085 1113
Size: 808 277 28
Fragments arranged by size:
808 277 28
MslI CAynn'nnrTG
Cuts at: 0 602 629 1113
Size: 602 27 484
Fragments arranged by size:
602 484 27
NaeI GCC'GGC
Cuts at: 0 1104 1113
Size: 1104 9
NarI GG'CG_CC
Cuts at: 0 900 1113
Size: 900 213
NgoMIV G'CCGG_C
Cuts at: 0 1102 1113
Size: 1102 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 192 1113
Size: 42 150 921
Fragments arranged by size:
921 150 42
PfoI T'CCnGG_A
Cuts at: 0 916 1113
Size: 916 197
PspOMI G'GGCC_C
Cuts at: 0 23 1033 1113
Size: 23 1010 80
Fragments arranged by size:
1010 80 23
SacI G_AGCT'C
Cuts at: 0 308 1060 1113
Size: 308 752 53
Fragments arranged by size:
752 308 53
SfoI GGC'GCC
Cuts at: 0 901 1113
Size: 901 212
SmaI CCC'GGG
Cuts at: 0 817 952 1113
Size: 817 135 161
Fragments arranged by size:
817 161 135
SmlI C'TyrA_G
Cuts at: 0 729 1051 1113
Size: 729 322 62
Fragments arranged by size:
729 322 62
StyI C'CwwG_G
Cuts at: 0 588 1113
Size: 588 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 971 1113
Size: 971 142
TatI w'GTAC_w
Cuts at: 0 375 1113
Size: 375 738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 1113
Size: 256 32 825
Fragments arranged by size:
825 256 32
Tth111I GACn'n_nGTC
Cuts at: 0 100 938 1113
Size: 100 838 175
Fragments arranged by size:
838 175 100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 1068 1113
Size: 318 750 45
Fragments arranged by size:
750 318 45
XmaI C'CCGG_G
Cuts at: 0 815 950 1113
Size: 815 135 163
Fragments arranged by size:
815 163 135
XmnI GAAnn'nnTTC
Cuts at: 0 482 1113
Size: 482 631
Enzymes that do cut:
AcuI AhdI AleI ApaI ApoI AvaI BanI BanII
BbeI BbvCI BclI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BsrBI BsrDI BsrFI BstAPI BstXI BstYI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII
KasI MmeI MslI NaeI NarI NgoMIV PflMI PfoI
PspOMI SacI SfoI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaWI BsgI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MscI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI ZraI