Restriction map for
pF1KB6552
(Linear) (Six-base) MAPSORT of: KIBB6552.seq Check: 7385 from: 1 to: 1182
KIBB6552.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 320 1182
Size: 320 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1080 1182
Size: 945 135 102
Fragments arranged by size:
945 135 102
AleI CACnn'nnGTG
Cuts at: 0 214 1182
Size: 214 968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 151 1182
Size: 151 1031
AlwNI CAG_nnn'CTG
Cuts at: 0 258 390 554 770 981 1002 1182
Size: 258 132 164 216 211 21 180
Fragments arranged by size:
258 216 211 180 164 132 21
ApaLI G'TGCA_C
Cuts at: 0 832 1120 1182
Size: 832 288 62
Fragments arranged by size:
832 288 62
ApoI r'AATT_y
Cuts at: 0 466 1182
Size: 466 716
AvaI C'yCGr_G
Cuts at: 0 1038 1182
Size: 1038 144
AvrII C'CTAG_G
Cuts at: 0 183 1182
Size: 183 999
BanI G'GyrC_C
Cuts at: 0 103 1182
Size: 103 1079
BanII G_rGCy'C
Cuts at: 0 238 533 1182
Size: 238 295 649
Fragments arranged by size:
649 295 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 1086 1182
Size: 1086 96
BglI GCCn_nnn'nGGC
Cuts at: 0 845 1182
Size: 845 337
Bme1580I G_kGCm'C
Cuts at: 0 836 1124 1182
Size: 836 288 58
Fragments arranged by size:
836 288 58
BmgBI CAC'GTC
Cuts at: 0 1010 1182
Size: 1010 172
BmrI ACTGGGnnnn_n'
Cuts at: 0 512 730 1182
Size: 512 218 452
Fragments arranged by size:
512 452 218
BmtI G_CTAG'C
Cuts at: 0 1034 1182
Size: 1034 148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 928 1182
Size: 268 660 254
Fragments arranged by size:
660 268 254
Bpu10I CC'TnA_GC
Cuts at: 0 230 1182
Size: 230 952
BsaI GGTCTCn'nnnn_
Cuts at: 0 1113 1182
Size: 1113 69
BsaHI Gr'CG_yC
Cuts at: 0 653 1182
Size: 653 529
BsaWI w'CCGG_w
Cuts at: 0 1104 1182
Size: 1104 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 751 781 1155 1182
Size: 751 30 374 27
Fragments arranged by size:
751 374 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 283 286 675 925 1182
Size: 28 255 3 389 250 257
Fragments arranged by size:
389 257 255 250 28 3
BseYI C'CCAG_C
Cuts at: 0 1132 1174 1182
Size: 1132 42 8
Fragments arranged by size:
1132 42 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1135 1182
Size: 403 732 47
Fragments arranged by size:
732 403 47
BsiEI CG_ry'CG
Cuts at: 0 859 1182
Size: 859 323
BsiHKAI G_wGCw'C
Cuts at: 0 44 836 1124 1182
Size: 44 792 288 58
Fragments arranged by size:
792 288 58 44
Bsp1286I G_dGCh'C
Cuts at: 0 44 238 533 836 1124 1182
Size: 44 194 295 303 288 58
Fragments arranged by size:
303 295 288 194 58 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1157 1182
Size: 1157 25
BsrBI CCG'CTC
Cuts at: 0 122 1056 1182
Size: 122 934 126
Fragments arranged by size:
934 126 122
BssHII G'CGCG_C
Cuts at: 0 339 1182
Size: 339 843
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 390 981 1182
Size: 258 132 591 201
Fragments arranged by size:
591 258 201 132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 873 1182
Size: 489 384 309
Fragments arranged by size:
489 384 309
BstYI r'GATC_y
Cuts at: 0 254 593 1182
Size: 254 339 589
Fragments arranged by size:
589 339 254
BtgI C'CryG_G
Cuts at: 0 99 816 1112 1182
Size: 99 717 296 70
Fragments arranged by size:
717 296 99 70
BtsI GCAGTG_nn'
Cuts at: 0 42 624 834 1182
Size: 42 582 210 348
Fragments arranged by size:
582 348 210 42
ClaI AT'CG_AT
Cuts at: 0 797 1182
Size: 797 385
EaeI y'GGCC_r
Cuts at: 0 860 1004 1182
Size: 860 144 178
Fragments arranged by size:
860 178 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 928 945 1080 1182
Size: 268 660 17 135 102
Fragments arranged by size:
660 268 135 102 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1182
Size: 355 827
EcoO109I rG'GnC_Cy
Cuts at: 0 486 560 583 1042 1182
Size: 486 74 23 459 140
Fragments arranged by size:
486 459 140 74 23
EcoRI G'AATT_C
Cuts at: 0 466 1182
Size: 466 716
HaeII r_GCGC'y
Cuts at: 0 391 939 1182
Size: 391 548 243
Fragments arranged by size:
548 391 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 1182
Size: 751 32 399
Fragments arranged by size:
751 399 32
HincII GTy'rAC
Cuts at: 0 195 1182
Size: 195 987
MscI TGG'CCA
Cuts at: 0 1006 1182
Size: 1006 176
MslI CAynn'nnrTG
Cuts at: 0 214 736 1182
Size: 214 522 446
Fragments arranged by size:
522 446 214
MspA1I CmG'CkG
Cuts at: 0 92 978 1178 1182
Size: 92 886 200 4
Fragments arranged by size:
886 200 92 4
NheI G'CTAG_C
Cuts at: 0 1030 1182
Size: 1030 152
NspI r_CATG'y
Cuts at: 0 648 1182
Size: 648 534
PasI CC'CwG_GG
Cuts at: 0 108 657 1182
Size: 108 549 525
Fragments arranged by size:
549 525 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 1002 1182
Size: 1002 180
PpuMI rG'GwC_Cy
Cuts at: 0 560 583 1042 1182
Size: 560 23 459 140
Fragments arranged by size:
560 459 140 23
PstI C_TGCA'G
Cuts at: 0 37 151 550 986 1182
Size: 37 114 399 436 196
Fragments arranged by size:
436 399 196 114 37
PvuII CAG'CTG
Cuts at: 0 978 1178 1182
Size: 978 200 4
Fragments arranged by size:
978 200 4
SanDI GG'GwC_CC
Cuts at: 0 560 1042 1182
Size: 560 482 140
Fragments arranged by size:
560 482 140
SfcI C'TryA_G
Cuts at: 0 33 147 546 982 1182
Size: 33 114 399 436 200
Fragments arranged by size:
436 399 200 114 33
StuI AGG'CCT
Cuts at: 0 916 1182
Size: 916 266
StyI C'CwwG_G
Cuts at: 0 183 482 533 1182
Size: 183 299 51 649
Fragments arranged by size:
649 299 183 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 180 1182
Size: 180 1002
TatI w'GTAC_w
Cuts at: 0 722 791 1182
Size: 722 69 391
Fragments arranged by size:
722 391 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1155 1182
Size: 1155 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 870 1182
Size: 531 339 312
Fragments arranged by size:
531 339 312
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaLI ApoI AvaI
AvrII BanI BanII BbsI BglI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BssHII
BstAPI BstXI BstYI BtgI BtsI ClaI EaeI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII MscI MslI
MspA1I NheI NspI PasI PflMI PpuMI PstI PvuII
SanDI SfcI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI AscI AseI AsiSI BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BplI BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI